哮喘小鼠脾细胞中粒细胞型髓源性抑制细胞和Th17细胞水平的变化

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Abstract Objective: To explore the level of myeloid-derived suppressor cells(MDSCs), Th17 and Treg cells in spleen cells of asthmatic mice. Methods: BALB/c male mice at age of 6-8 weeks were randomly divided into two groups: PBS group and model group. The group of asthmatic mice was established with OVA, and the ova-specific IgE in the mouse serum was detected by enzyme-linked immunosorbent assay (ELISA). HE staining and PAS staining were used to observe the pathological change in lung tissues. The levels of Th17, Treg and MDSCs in splenocyte were detected by flow cytometry. Immunohistochemical staining was used to observe the expression of Gr-1, IL-17 and Foxp3 in lung tissues of mice. Results: Compared with PBS group, airway inflammatory cells and mucus secretion were increased in the model group and serum IgE level was significantly increased. The proportion of Th17 cells in spleen cells increased and that of Treg cells decreased. The percentage of G-MDSCs subset in MDSCs was increased. Meanwhile, IL-17 and Gr-1 were highly expressed and Foxp3 was low in lung tissue of model group. Conclusion: High proportion of G-MDSCs and Th17 cells, and low proportion of Treg may be contributed to pathogenesis of asthmatic mice.

Received: 05 February 2020

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	Articles by authors
	XUE Fei1
	2
	YU Meng-zhu1
	2
	SHAN Wenqi1
	2
	LU Hong-yan1
	WANG Xue-feng2

Cite this article:

XUE Fei1,2,YU Meng-zhu1等.

Changes of granulocytic myeloid-derived suppressor cell and Th17 cells in spleen cells of asthmatic mice

[J]. Journal of Jiangsu University(Medicine Edition), 2020, 30(05): 414-417.

URL:

http://zzs.ujs.edu.cn/xbyxb/EN/ OR http://zzs.ujs.edu.cn/xbyxb/EN/Y2020/V30/I05/414

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