不明原因发热患者肺泡灌洗液病毒群落解析

Abstract
Figure/Table
References (0)
Related Citation (0)

Download: PDF (3576 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract Objective: To analyze the virus profile in alveolar lavage fluid of patients with fever of unknown origin. Methods: A total of 58 samples of alveolar lavage fluid were randomly divided into five groups to construct 5 libraries. Virus metagenomics technology was used to analyze the virus community of five libraries, and PCR screening was performed for the obtained torque teno virus and rhinovirus. Results: The results of virus composition showed that the virus community was dominated by Anelloviridae, which accounted for 19.4% of the total population, followed by Circoviridae(11.0%), Phycodnaviridae(11.0%), Human endogenous retrovirus(10.2%), Poxviridae(4.9%), Retroviridae(3.2%),Virgaviridae(2.3%) and Picornaviridae(0.6%). Four strains of torque teno virus with long sequences were found, and phylogenetic analysis was carried out according to open reading frame 1 of torque teno virus, which showed that it was different from the known virus in some sequences. The positive rate of torque teno virus and rhinovirus was 11.3% and 10.0% respectively by PCR screening. Conclusion: The viral community composition in alveolar lavage fluid of patients with fever of unknown origin was determined with virus metagenomics technology. The Anelloviridae had the highest proportion in the virus community, in which the torque teno virus proliferated due to the inflammatory environment caused by fever and the low immunity of patients.

Received: 21 February 2020

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	LI Xin-lin1
	LIU Qi1
	CHEN Xu1
	XIAO Yu-qing1
	YANG Shi-xing1
	ZHANG Wen1
	CHEN Jian-guo2

Cite this article:

LI Xin-lin1,LIU Qi1,CHEN Xu1等.

Analysis of viral community in bronchoalveolar lavage fluid of patients with fever of unknown origin

[J]. Journal of Jiangsu University(Medicine Edition), 2020, 30(05): 395-401.

URL:

http://zzs.ujs.edu.cn/xbyxb/EN/ OR http://zzs.ujs.edu.cn/xbyxb/EN/Y2020/V30/I05/395

［1］Cunha BA, Lortholary O, Cunha CB. Fever of unknown origin: a clinical approach\[J\]. Am J Med, 2015, 128(10): 1138.e1-1138.e15.

［2］嵇玮嘉, 颜学兵. 不明原因发热流行病学及临床特征分析\[J\]. 中华医院感染学杂志, 2019, 29(24): 3681-3686.

［3］Kazanjian PH. Fever of unknown origin: review of 86 patients treated in community hospitals\[J\]. Clin Infect Dis, 1992, 15(6): 968-973.

［4］Zhang W, Li L, Deng X, et al. Viral nucleic acids in human plasma pools\[J\]. Transfusion, 2016, 56(9): 2248-2255.

［5］Nishizawa T, Sugimoto Y, Takeda T, et al. Identification and whole genome characterization of novel anelloviruses in masked palm civets (Paguma larvata): Segregation into four distinct clades\[J\]. Virus Res, 2018, 256:183-191.

［6］李旺. 鼠肠道病毒宏基因组学研究\[D\]. 镇江: 江苏大学, 2016.

［7］Biagini P. Classification of TTV and related viruses (anelloviruses)\[J\]. Curr Top Microbiol Immunol, 2009, 331:21-33.

［8］Bernardin F, Operskalski E, Busch M, et al. Transfusion transmission of highly prevalent commensal human viruses\[J\]. Transfusion, 2010, 50(11): 2474-2483.

［9］郭靓骅, 吴孔菊, 华修国, 等. 病毒宏基因组学方法鉴定出小熊猫粪便中一株新型指环状病毒\[J\].上海交通大学学报(农业科学版),2018,36(1):14-19,35.

［10］Nishiyama S, Dutia BM, Stewart JP, et al. Identification of novel anelloviruses with broad diversity in UK rodents\[J\]. J Gen Virol, 2014, 95(7): 1544-1553.

［11］Martelli F, Macera L, Spezia PG, et al. Torquetenovirus detection in exosomes enriched vesicles circulating in human plasma samples\[J\]. Virol J, 2018, 15(1): 145.

［12］De Villiers EM, Borkosky SS, Kimmel R, et al. The diversity of torque teno viruses: in vitro replication leads to the formation of additional replicationcompetent subviral molecules\[J\]. J Virol, 2011, 85(14): 7284-7295.

［13］McElvania TeKippe E, Wylie KM, Deych E, et al. Increased prevalence of anellovirus in pediatric patients with fever\[J\]. PLoS One, 2012, 7(11): e50937.

［14］周涛, 贾怀杰, 何小兵, 等. 人兽共患痘病毒病流行现状及其宿主谱研究进展\[J\]. 中国人兽共患病学报, 2012, 28(2): 159-163,166.

［15］张桂菊, 赵红梅, 吴金勇, 等. 中西医结合治疗小儿流感病毒性肺炎临床疗效观察\[J\]. 山东医药, 2015, 55(5): 30-31.

［16］Yan F, Xiao Y, Li M, et al. Metagenomic analysis identified human rhinovirus B91 infection in an adult suffering from severe pneumonia\[J\]. Am J Respir Crit Care Med, 2017, 195(11): 1535-1536.

［17］Lewandowska DW, Schreiber PW, Schuurmans MM, et al. Metagenomic sequencing complements routine diagnostics in identifying viral pathogens in lung transplant recipients with unknown etiology of respiratory infection\[J\]. PLoS One, 2017, 12(5): e0177340.

［18］Maggi F, Pifferi M, Tempestini E, et al. TT virus loads and lymphocyte subpopulations in children with acute respiratory diseases\[J\]. J Virol, 2003, 77(16): 9081-9083.

［19］Pou C, BarrientosSomarribas M, MarinJuan S, et al. Virome definition in cerebrospinal fluid of patients with neurological complications after hematopoietic stem cell transplantation\[J\]. J Clin Virol, 2018, 108:112-120.

［20］Blazsek A, Sillo P, Ishii N, et al. Searching for foreign antigens as possible triggering factors of autoimmunity: Torque Teno virus DNA prevalence is elevated in sera of patients with bullous pemphigoid\[J\]. Exp Dermatol, 2008, 17(5): 446-454.

［21］Zhang Z, Dai W, Dai D. Molecular characterization of pigeon torque teno virus (PTTV) in Jiangsu province\[J\]. Comput Biol Chem, 2017, 69:10-18.

［22］Kapusinszky B, Phan TG, Kapoor A, et al. Genetic diversity of the genus Cosavirus in the family Picornaviridae: a new species, recombination, and 26 new genotypes\[J\]. PLoS One, 2012, 7(5): e36685.

［23］Woo PC, Lau SK, Li T, et al. A novel dromedary camel enterovirus in the family Picornaviridae from dromedaries in the Middle East\[J\]. J Gen Virol, 2015, 96(Pt7): 1723-1731.