CXCL12-CXCR4轴促进胶质母细胞瘤前神经间质转化

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Abstract [Abstract]Objective: To investigate the effect of CXC chemokine ligand 12CXC chemokine receptor 4 (CXCL12CXCR4) axis on proneuralmesenchymal transition in glioblastoma. Methods: The difference of CXCR4 mRNA in glioma molecular subtyping tumor tissues and its effect on tumor patients′ survival rate were analyzed based on data in the TCGAGBM gene database; human glioma LN428 and U87MG cells were pretreated with different concentrations of CXCL12(0, 20, 40, 60, 80, 100 ng/mL) and 20 μmol/L plerixafor (selective CXCR4 antagonist) for 48 h, the expression levels of OLIG2, Ecadherin, YKL40, Ncadherin and Vimentin were detected by Western blotting, the cell migration and invasion ability were detected by Transwell assay, the proliferation ability was detected by CCK8 assay and colony formation assay. Results: According to the data in the TCGAGBM database, compared with normal group, the mRNA expressions of CXCR4 in glioma patients was significantly increased, and it was negatively correlated with the survival rate of patients. The mRNA expression level of CXCR4 in mesenchymal glioblastoma tissue samples was significantly higher than that in proneural glioblastoma (all P<0.05). Compared with control group, the expressions of mesenchymalassociated proteins YKL40, Ncadherin and Vimentin were significantly increased in CXCL12 group, while the expressions of the proneuralrelated proteins OLIG2, Ecadherin were markedly decreased, migration and invasion ability and proliferative ability of glioma LN428 and U87MG cells were greatly enhanced (all P<0.05). After treatment with plerixafor, migration and invasion ability and proliferative ability of glioma LN428 and U87MG cells were remarkably inhibited, expression of mesenchymalrelated proteins was highly decreased, and expression of proneuralrelated proteins was increased (all P<0.05). Conclusion: The CXCL12CXCR4 axis promotes proneuralmesenchymal transition, migration, invasion and proliferation of glioblastoma.

Received: 30 October 2019

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	Articles by authors
	XU Xue-wen1
	Song Lian1
	YANG Xiao-xiao1
	SHI Hui1
	GONG Ai-hua2
	WANG Ming1
	ZHANG Li-rong1

Cite this article:

XU Xue-wen1,Song Lian1,YANG Xiao-xiao1等.

CXCL12-CXCR4 axis promotes proneural-mesenchymal transition in glioblastoma

[J]. Journal of Jiangsu University(Medicine Edition), 2020, 30(01): 18-23.

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http://zzs.ujs.edu.cn/xbyxb/EN/ OR http://zzs.ujs.edu.cn/xbyxb/EN/Y2020/V30/I01/18

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