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The roles of malic enzyme 3 knockdown in mitochondrial fusion and fission in human glioma cells |
CHENG Jie1, CHEN Xi2, TANG Yu1, LIU Fei1, GONG Ai-hua1 |
(1. School of Medicine, Jiangsu University, Zhenjiang Jiangsu 212013; 2. Jiangsu Nursing Vocational School,Huaian Jiangsu 223005, China) |
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Abstract Objective: To investigate the effects of malic enzyme 3(ME3) on the ability of mitochondrial fusion and fission in human glioma cells. Methods: The sh-ME3 plasmids and sh-EGFP(control plasmids ) were transfected into the glioma cell LN229. The plasmid knockdown efficiency were detected by real time PCR and Western blotting. Western blotting was used to detect the markers of apoptosis and mitochondrial fusion and fission, and membrane potential of mitochondria was detected by fluorescent probe. Results: Compared with the control group, the mRNA expression level of ME3 in the experimental group decreased by 76%(t=20.16 ,P<0.01),and the protein expression level also decreased; the mitochondrial fission related markers Fis-1 and DRP1 were increased, while the mitochondrial fusion related markers Mfn1, Mfn2, OPA1 were decreased, membrane potential of mitochondria was decreased; the apoptosis related marker cleaved-caspase 3 was increased,while Bcl-2 was decreased. Conclusion: The knockdown of ME3 promotes mitochondrial fission in glioma cells, inhibits their fusion, reduces membrane potential of mitochondria, thereby causes apoptosis.
[Key words]malic enzyme 3; glioma; mitochondrial; apoptosis
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Received: 13 March 2019
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