6对通用引物检测不同型别人乳头瘤病毒效果分析

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Abstract Objective: To compare the detection efficacy of 6 universal primers in detecting 9 different types of human papillomavirus (HPV) and provide a quick and easy screening method for clinical studies. Methods: We collected 954 female cervical exfoliated cell samples, then detected and classified the HPVDNA with HPV typing kit. Then we used 6 pairs of universal primers （①GP5/GP6，②GP5+/GP6+，③CP-Ⅰ/CP-ⅡS，④CP-I/CP-ⅡG，⑤external：MY09/MY11，internal：GP5+/GP6+，⑥SPF1/GP6++）to detect HPVDNA positive samples by PCR and compared their detection effect in 9 types of HPV-DNA. Results: A total of 263 HPV-DNA positive samples were detected from 954 specimens with HPV typing kit， the HPV infection rate was 27.57%. In the HPVDNA positive samples, the positive cases detected by SPF1/GP6++ was 223, and the detection rate was 84.79%. But 207 positive cases were detected by nested PCR primers (external: MY09/MY11, internal: GP5+/GP6+), the corresponding detection rate was 78.71%. The two universal primers above were able to detect all 9 HPVDNA types. Conclusion: The universal primer SPF1/GP6++ has the best detection efficacy, but the synthesis cost is higher because of containing hypoxanthine. The nest PCR primer (external: MY09/MY11, inner:GP5+/GP6+) has the second highest detection efficacy, and the synthesis cost is lower. Therefore, we may select suitable universal primers according to the actual situation in HPV detection.

Received: 21 May 2019

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