莫诺苷对小鼠骨髓源树突状细胞表型及功能的影响

Abstract
Figure/Table
References
Related Citation (4)

Download: PDF (1757 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract Objective: To investigate the effects of morroniside on the differentiation, phenotype and function of murine bonemarrow derived dendritic cells (BMDCs) in vitro. Methods: Primary murine BMDCs were prepared and divided into four groups: normal control group, morronisidetreated group, LPStreated group and morroniside+LPStreated group. The changes of BMDCs morphology and fluorescence intensity of CFSE were confirmed respectively with light microscopy and flow cytometry (FCM) . The phenotype and functional maturation of BMDCs and activation of antigenspecific CD4+ T cell proliferation and differentiation under influence of morroniside were evaluated by FCM. ELISA was used for analyzing the protein levels of IL6/IL12 in supernatant of cultured BMDCs. Results: Compared with the normal control group, there was no significant difference in the proliferation efficiency of BMDCs induced differentiation after morroniside treatment. Compared with the LPStreated group, morroniside combined with LPS could upregulated the expression of CD80,CD86, MHCⅠ and MHCⅡ molecules and promoted the IL12 secretion on BMDCs. Furthermore, morroniside treatment promoted activation,proliferation and differentiation priming capability on OVA323-339 antigenspecific T cells of BMDCs. Conclusion: Morroniside effectively enhances the maturation of BMDCs and upregulates the antigenpresenting function of OVAspecific CD4+ T cells in vitro.

Received: 18 March 2019

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors

Cite this article:

URL:

http://zzs.ujs.edu.cn/xbyxb/EN/ OR http://zzs.ujs.edu.cn/xbyxb/EN/Y2019/V29/I04/277

［1］Wang W, Xu J, Li L, et al. Neuroprotective effect of morroniside on focal cerebral ischemia in rats\[J\]. Brain Res Bull, 2010, 83(5): 196-201.
［2］Sun FL, Wang W, Zuo W, et al. Promoting neurogenesis via Wnt/βcatenin signaling pathway accounts for the neurorestorative effects of morroniside against cerebral ischemia injury\[J\]. Eur J Pharmacol, 2014, 738: 214-221.
［3］Sun FL, Wang W, Cheng H, et al. Morroniside improves microvascular functional integrity of the neurovascular unit after cerebral ischemia\[J\]. PLoS One, 2014, 9(6): e101194.
［4］Xu HQ, Hao HP, Zhang X, et al. Morroniside protects cultured human umbilical vein endothelial cells from damage by high ambient glucose\[J\]. Acta Pharmacol Sin, 2004, 25(4): 412-415.
［5］Wang W, Huang W, Li L, et al. Morroniside prevents peroxideinduced apoptosis by induction of endogenous glutathione in human neuroblastoma cells\[J\]. Cell Mol Neurobiol, 2008, 28(2): 293-305.
［6］Hu N, Ren S, Li W, et al. Morroniside promotes bone marrow mesenchymal stem cell proliferation in rats\[J\]. Mol Med Rep, 2013, 7(5): 1565-1570.
［7］Xia S, Guo Z, Xu X, et al. Hepatic microenvironment programs hematopoietic progenitor differentiation into regulatory dendritic cells, maintaining liver tolerance\[J\]. Blood, 2008, 112(8): 3175-3185.
［8］Yu B, Zhang G, An Y, et al. Morroniside on antiinflammation activities in rats following acute myocardial infarction\[J\]. Korean J Physiol Pharmacol, 2018, 22(1): 17-21.
［9］Zhang S, Wang Q. Factors determining the formation and release of bioactive IL12: regulatory mechanisms for IL12p70 synthesis and inhibition\[J\]. Biochem Biophys Res Commun, 2008, 372(4): 509-512.
［10］Jones LL, Vignali DA. Molecular interactions within the IL6/IL12 cytokine/receptor superfamily\[J\]. Immunol Res, 2011, 51(1): 5-14.
［11］Gee K, Guzzo C, Che Mat NF, et al. The IL12 family of cytokines in infection, inflammation and autoimmune disorders\[J\]. Inflamm Allergy Drug Targets, 2009, 8(1): 40-52.
［12］Vignali DA, Kuchroo VK. IL12 family cytokines: immunological playmakers\[J\]. Nat Immunol, 2012, 13(8): 722-728.
［13］Barnes PJ, Karin M. Nuclear factorκB: a pivotal transcription factor in chronic inflammatory diseases\[J\]. N Engl J Med, 1997, 336(15): 1066-1071.
［14］Yamamoto Y, Gaynor RB. Therapeutic potential of inhibition of the NFκB pathway in the treatment of inflammation and cancer\[J\]. J Clin Invest, 2001, 107(2): 135-142.
［15］Zhang Z, Liong EC, Lau TY, et al. Induction of apoptosis by hexamethylene bisacetamide is p53dependent associated with telomerase activity but not with terminal differentiation\[J\]. Int J Oncol, 2000, 16(5): 887-892.
［16］Park CH, Yamabe N, Noh JS, et al. The beneficial effects of morroniside on the inflammatory response and lipid metabolism in the liver of db/db mice\[J\]. Biol Pharm Bull, 2009, 32(10): 1734-1740.