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| Ginkgol C171 and cisplatin co-treatment reversed A549/DDP cells resistance to cisplatin |
| DONG Yan1， LI Yue-ying1, ZHANG Xin-chi1, YANG Xiao-ming2, LI lin-jie1, MA Wenbin1 |
| 1. School of Medicine, 2. School of Food Science and Biological Engineering, Jiangsu University, Zhenjiang Jiangsu 212013, China) |
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Abstract Objective: To explore the effect of ginkgol C171 on the antitumor drug resistance and apoptosis of cisplatin resistant lung adenocarcinoma (A549/DDP) cells. Methods: A549 and A549/DDP cells were cultured in vitro, and MTT assay was used to detect the drug resistance of A549/DDP cells and the optimal concentration of cisplatin. After treatment with cisplatin of the aboveoptimal concentration and various concentrations of ginkgol C171 (0,10, 20, 40 mg/L), the migration, invasion, resistancerelated proteins and apoptosis rate of A549/DDP cells were detected by Transwell assay, Western blotting and flow cytometry, respectively. Results: MTT result showed the resistance of A549/DDP cells was 5.8 times vs A549 cells, and the optimal concentration of cisplatin was 4 mg/L. Compared with the control group, Transwell assay demonstrated that the migration and invasion of A549/DDP cells were significantly inhibited in ginkgol C171 at different concentrations and cisplatin combination groups(P<0.05). Western blotting showed that the expressions of Nrf2, Mrp1 and antiapoptotic protein Bcl2 were inhibited(P<0.05), while apoptotic protein Bax and Keap1 protein were increased(P<0.01) when A549/DDP cells were cotreated with different concentrations of ginkgol C171 and cisplatin. Cytometry demonstrated that the apoptosis rates of A549/DDP cells were increased significantly by different concentrations of ginkgol C171 and cisplatin cotreatment groups(P<0.05). Conclusion: Ginkgol C171 could reverse the resistance of A549/DDP cells to cisplatin in vitro，and promote apoptosis.
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Received: 16 October 2017
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2018, Vol. 28 
