rL-IL29对肺腺癌A549细胞生物学特性的影响及相关机制

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Abstract Objective: To investigate the effect of recombinant Newcastle disease virus expressing IL29（rLIL29） on the proliferation, migration and invasion of lung adenocarcinoma A549 cells and explore the potential mechanism. Methods: A549 cells were cultured in vitro, then infected by rLIL29, Newcastle disease virus LoSota(NDVL) or treated by PBS. The expression of NDV HN and IL29 protein in cells was detected by Western blotting method.The effects of rL-IL29 on the proliferation, migration and invasion of lung adenocarcinoma A549 cell lines were detected by CCK8, scratch test and Transwell invasion test.The expression level of E-cadherin, MMP2, vimentin and AKT/NF-κB pathway associated proteins were detected by Western blotting. Results: NDV and IL-29 protein were stablely expressed in A549 cells infected by rL-IL29, and significantly higher than those of NDVL and control group. After infected with rL-IL29 and NDVL, the ability of proliferation,migration and invasion of A549 cells was inhibited, and rL-IL29 group is more powerful than the control group and NDVL group.The expression level of E-cadherin in the A549 cells was significantly increased after rL-IL29 infection, and the expression of vimentin,MMP2 (66 kDa/72 kDa), p-AKT and P65 protein was all significantly decreased(all P＜0.05). Conclusion: rL-IL29 inhibited the proliferation,invasion and migration of lung adenocarcinoma A549 cells,and the mechanism may be up-regulation of of E-cadherin protein expression and downregulation of MMP2 and vimentin protein expression by inhibiting the AKT/NF-κB signaling pathway.

Received: 12 January 2018

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http://zzs.ujs.edu.cn/xbyxb/EN/ OR http://zzs.ujs.edu.cn/xbyxb/EN/Y2018/V28/I02/99

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