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| Luteolin inhibits enterovirus 71 induced cytopathic effects in vitro
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| YAN Xiao-yong1, LI Xiang2, WANG Xiao-li2, CHENG Jian-ping2, XIN Fei2, HU De-fei2, HAN Li2, JIN Yue2 |
| (1. Department of Clinical Laboratory, Huai′an First Hospital Affiliated to Nanjing Medical University, Huai′an Jiangsu 223300; 2. Department of Clinical Laboratory, Huai＇an Hospital Affiliated to Xuzhou Medical University, Huai′an Jiangsu 223301, China) |
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Abstract Objective： To investigate the mechanism of inhibition of enterovirus 71 (EV71) induced cytopathic effects by luteolin in vitro. Methods： Rhabdomyosarcoma(RD) cells were treated with 0, 25, 50, 100, 200, 400 μg/mL luteolin for 48 h, then cells viability was determined by MTT. Meanwhile, RD cells were divided into control group(DMEM treatment), luteolin group(25, 50, 100 μg/mL luteolin treatment), EV71 infection group and luteolin(25, 50, 100 g/mL)+ EV71 infection group. The cell viability was assessed by MTT assay. Apoptosis of RD cells was detected by flow cytometry. The expression of Caspase3 was detected by Western blotting. In addition, the concentration of proinflammatory cytokines were detected by ELISA. Results： Compared with 0 μg/mL luteolin, 25, 50 or 100 μg/mL luteolin had no significant effect on RD cell viability(P>0.05). The survival rates of RD cells in 25, 50 or 100 μg/mL luteolin + EV71 group were significantly higher than EV71 group(all P<0.05). Compared with EV71 group, the apoptosis rate, Caspase3 expression and the concentration of TNF-α, IL-1β, IL-6 and IL-10 were significantly decreased in luteolin+ EV71 group(all P<0.05). Conclusion： Luteolin inhibits EV71 infection induced cytopathic effects and these protection may be resulted from the inhibition of apoptosis and inflammatory cytokine secretion.
［Key words］luteolin; enterovirus 71; apoptosis; inflammatory cytokines
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Received: 17 October 2016
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2016, Vol. 26 
