内质网应激预处理对丙烯腈氧化性损伤的保护作用

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Abstract Objective: To explore the effects of pretreatment of endoplasmic reticulum stress on oxidative toxicity of acrylonitrile in rats. Methods: Thirty-six SD rats were randomly divided into six groups as follows: blank control group, 2-DG alone treated group, two acrylonitrile treated groups, 2-DG pretreated and acrylonitrile treated groups. The animals were intraperitoneally injected daily with 2-DG at the dosage of 100 mg/kg for 1 week,then rats were intraperitoneally injected with acrylonitrile(50,75 mg/kg), and they were decapitated 1 h after the last administration of acrylonitrile or normal saline. The brain and liver tissues were immediately dissected out and weighted on ice.The expression of glucose-regulated protein-78(Grp78) was determined by Western blotting. Oxidative toxicity of acrylonitrile was assessed by the reduced glutathione levels(GSH), products of lipid peroxidation(MDA), and superoxide dismutase (SOD) activity in the brain and liver. Results: Levels of protein expression of Grp78 increased after pretreatment with 2-DG compared with that of control group(P＜0.05). A significant increase in levels of MDA was observed in the acrylonitrile-treated group both in the brain and liver compared with the control group(P<0.05). These increases were accompanied by a significant decrease in GSH content and a significant reduction in SOD activity in the same tissues (P＜0.05).However, when rats were pretreated with 2-DG,increase of MDA and decrease of GSH contents and SOD activity was significantly attenuated both in liver and brain, compared with acrylonitrile administration alone. Conclusion: Pretreatment with 2-DG reversed the acrylonitrile-induced effects by reducing the levels of MDA and enhancing SOD activity and increasing GSH content both in the brain and liver.

Received: 29 April 2014

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http://zzs.ujs.edu.cn/xbyxb/EN/ OR http://zzs.ujs.edu.cn/xbyxb/EN/Y2014/V24/I04/302

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