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| Expression and role of S100B on the injury of cardiomyocytes induced by doxorubicin |
| HAN Yao-hui, PAN Hao, MAO Jun-qian, ZHOU Yan-fang, ZHANG Ying-yu, WANG Hao, BAO Yong-hui, ZHAO Long, LIU Ling-ling, ZHANG Guo-hui |
| Department of Cardiology, the Affiliated People′s Hospital of Jiangsu University, Zhenjiang Jiangsu 212002, China |
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Abstract Objective: To investigate the expression and the role of S100B on the injury of cardiomyocytes induced by doxorubicin. Methods: The cardiomyocytes from 1d SD rats were cultured in DMEMF12 medium and then randomly divided into 4 groups: control group(CON), doxorubicin damnified group(DOX), doxorubicin+negatived small interfering RNA control group(DNC), and doxorubicin+S100B small interfering RNA group(DSB). S100B small interfering RNA was mediated by lipofectamineRNiMAX to transfect cardiomyocytes. The cardiomyocytes were treated with doxorubicin(2 μmol/L)for 2 h at 48 h posttransfection. The survival rate of cardiomyocytes was evaluated by MTT. The apoptosis rate was determined by flow cytometry(FCM). The expression of S100B was detected by Western blot. Results: MTT assay showed that there was no significant difference in the survival rate of cardiomyocytes between DOX group and DNC group(P>0.05). Compared with DOX group, the survival rate of cardiomyocytes in the DSB group was increased(P<0.05); Flow cytometry showed that there was no significant difference in the apoptosis rate of cardiomyocytes between DOX group and DNC group(P>0.05). Compared with DOX group, the apoptosis rate of cardiomyocytes in the DSB group was descended(P<0.01); Western blot showed that there was no significant difference in the expression of S100B between DOX group and DNC group(P>0.05), Compared with DOX group, the expression of S100B in the DSB group was descended(P<0.01). Conclusion: The expression of S100B were significantly increased on the injury of cardiomyocytes induced by doxorubicin and might promote the apoptosis.
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Received: 13 February 2013
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2013, Vol. 23 
