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| Expression and clinical significance of melatonin receptor MT1 and transcription factor Sp1 in breast cancer |
| ZENG Jian1, CHEN Li-ping1, CHEN Miao1, CHEN Yan2, XU Hao-qin2, DI Rong-ke1 |
| (1.School of Medical Science and Laboratory Medicine, Jiangsu University, Zhenjiang Jiangsu, 212013； 2.Zhenjiang Municipal Commission of Population and Family Planning, Zhenjiang Jiangsu 212001, China) |
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Abstract Objective: To explore the relationship and clinical significance between the expressions of melatonin receptor MT1 and transcription factor Sp1 in breast carcinoma. Methods： The expressions of MT1 and Sp1 in 90 specimens of breast cancer tissues and 50 specimens of adjacent normal breast tissues were detected by immunohistochemical streptavidin-peroxidase (SP) assay and western blotting. Results：There were significant differences in positive expression rates of MT1 and Sp1 between breast cancer tissues and adjacent normal breast tissues (64.4% vs 22.0%, P<0.01 for MT1, respectively, and 74.4% vs 28.0% P<0.01 for Sp1, respectively).The results of western blotting were in accordance with the results of immunohistochemistry technique. The expressions of MT1 and Sp1 in breast cancer tissues were correlated with TNM stage, tumor invasion and lymphnode metastasis(P<0.05), but not correlated with age of patients, tumor size and histological grade(P>0.05). The MT1 expression positively correlated with Sp1 expression in breast cancer tissues(r= 0.523，P<0.01). Conclusion：MT1 and Sp1 may be involved in the invasion and metastasis of breast cancer. The detection of both two expressions is valuable for judging clinical development and presuming prognosis.
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Received: 04 December 2012
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2013, Vol. 23 
