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| Effects of vitrifying mouse embryos from different periods and different sources |
| LIU Yun1, HU Jie2, CHEN Xiao-fang1, YIN Zhao-hui1, MI Wu-xuan1, HU Jia-bo2 |
| （1.Human Reproduction Medicine Center, the Fourth Affiliated Hospital of Jiangsu University, Zhenjiang Jiangsu 212001; 2.School of Medical Science and Laboratory Medicine, Jiangsu University, Zhenjiang Jiangsu 212013, China） |
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Abstract Objective: To compare the postthaw survival rate and the success rate in continuing to grow to blastocyst of vitrified mouse embryos from different periods and different sources. Methods: The 2-cell, 4-cell, 8-cell period mouse embryos were obtained through fertilization in vitro and in vivo respectively. These embryos were vitrified and thawed. We got the rate of their survival and blastocyst formation. And, we took those fresh embryos as the control group. Results: The survival rate of blastocyst formation of post-thaw 2-cell, 4-cell and 8-cell period embryos from fertilization in vitro and in vivo were 70.67%/65.33% and 72.06%/67.62%, 88.00%/81.33% and 89.71%/83.82% and 93.33%/92.00% and 94.12%/94.12% respectively. The blastocyst formation rate in the phrase of 2-cell and 4-cell frozen embryos was lower than the control group (P＜0.05).No significant difference was found between the group of 8-cell frozen embryos and the control group. In addition, fertilization modes had no significant effect on the survival rate and the blastocyst formation rate(P>0.05). Conclusion: Vitrification technology is an effective method in preserving mouse embryos. And the 8-cell period embryos might have the best cryopreservation effect.
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Received: 08 January 2013
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2013, Vol. 23 
