|
|
|
| Effect of gene NDRG1 transfection on the expression of COX-2 and VEGF and proliferation in human cervical cancer cells |
|
QIAN Hua1, WANG Hua1*, YUAN Dong-lan1, CUI Yong-an2, LIN Mei2, XIAO Wei3, JIAO Xia3， YU Hong3 |
| (1.Department of Gynaecology, 2.Department of Oncology, 3.Department of Pathology, Taizhou People′s Hospital, Taizhou Jiangsu 225300, China) |
|
|
|
|
Abstract Objective: To investigate the effect of N-myc downstream regulated gene 1(NDRG1) on the proliferation in human cervical cancer cell line SiHa, with a focus on the expression of cyclooxygenase-2(COX-2) and vascular endothelial growth factor(VEGF). Methods： pcDNA3.0-NDRG1 and pcDNA3.0 were transfected into SiHa cells through lipofectamine and positive clones were screened by G418. RT-PCR and Western blot were employed to identify mRNA and protein expression of NDRG1 in SiHa cells, respectively. The expression of COX-2 and VEGF in positive clones was detected by Western blot. Cell viability was assessed by MTT method. Results： Positive clone N-12 with NDRG1 overexpression was successfully established. Compared with non-transfected control group, the expression of COX-2 and VEGF was decreased significantly, and the proliferation level of N-12 was also decreased significantly. Conclusion： It is possible that NDRG1 could inhibit the development of cervical cancer by downregulating the expression of COX-2 and VEGF and the proliferation in SiHa cells.
|
|
Received: 02 July 2012
|
|
|
|
| [1] Tschan MP，Shan D，Laedrach J，et al.NDRG1/2 expression is inhibited in primary acute myeloid leukemia[J].Leuk Res，2010，34(3)：393-398.[2] Kim A，Kim MJ，Yang Y，et al.Suppression of NF-kappaB activity by NDRG2 expression attenuates the invasive potential of highly malignant tumor cells[J].Carcinogenesis，2009，30(6)：927-936.[3] 恽栋，李慧，于鸿，等. Smad7基因转染后人胰腺癌细胞uPA及PAI-1表达的变化[J]. 江苏大学学报：医学版，2010，20（6）：492-495.[4] 许斌，陈寅，戴桂红，等. 转染CTGF基因对乳腺癌细胞MMP-2及TIMP-2表达的影响[J].江苏大学学报：医学版，2012，22（2）:97-100.[5] 于鸿，黄俊星，王朝夫，等. Smad7反义寡核苷酸对胰腺癌SW1990细胞MMP-2和TIMP-2表达及细胞增殖的影[J].中华胰腺病杂志，2012，12（1）：11-14.[6] Kovacevic Z, Sivagurunathan S, Mangs H, et al. The metastasis suppressor, N-myc downstream regulated gene 1(NDRG1), upregulates p21 via p53-independent mechanisms[J]. Carcinogenesis,2011,32(5): 732-740.[7] Herrera FG, Chan P, Doll C, et al. A prospective phase III trial of the cyclooxygenase2 inhibitor celecoxib inpatients with carcinoma of the cervix with biomarker assessment of the tumormicroenvironment[J].Int J Radiat Oncol Biol Phys,2007,67(1): 97-103.[8] Saldivar JS, Lopez D, Feldman RA, et al. COX-2 overexpression as a biomarker of early cervical carcinogenesis:a pilot study[J]. Gynecol Oncol, 2007,107(1 Suppl 1):S155-162.[9] Wang AH,Tian XY,Yu JJ,et al. Celecoxib radiosensitizes the human cervical cancer HeLa cell line via amechanism dependent on reduced cyclooxygenase2 and vascular endothelialgrowth factor C expression[J]. J Int Med Res, 2012,40(1):56-66.[10] Yang F, Jin C, Jiang YJ, et al. Potential role of soluble VEGFR-1 in antiangiogenesis therapy for cancer[J]. Expert Rev Anticancer Ther,2011,11(4):541-549.[11] Zhang AH，Rao JN，Zou T，et al.p53-dependent NDRG1 expression induces inhibition of intestinal epithelial cell proliferation but not apoptosis after polyamine depletion[J].Am J Physiol Cell Physiol，2007，293(1)：C379-389.[12] Said HM，Stein S，Hagemann C，et al.Oxygen-dependent regulation of NDRG1 in human glioblastoma cells in vitro and in vivo[J].Oncol Rep，2009，21(1)：237-246. |
|
|
|
2012, Vol. 22 
