Effects of febuxostat on Treg/Th17 balance and vascular endothelialfunction in rats with hyperuricemia
CHEN Ning 1, SUN Hong 2, ZHENG Weiping 1, ZHOU Meng 3, LIU Jianwen 3, YAN Qing 3, GAO Fei 3, LIN He 3
（1. Department of Internal Medicine,Fujian Provincial Jinshan Hospital, Fuzhou Fujian 350001; 2. Department of Pharmacy, Fujian Provincial Hospital, Fuzhou Fujian 350001; 3. Department of Rheumatology and Immunology, Fujian Provincial Hospital, Fuzhou Fujian 350001, China）
Abstract：Objective: To investigate the effects of febuxostat on Treg/Th17 balance and vascular endothelial function in hyperuricemia rats. Methods: Thirtytwo male SD rats were randomly divided into 4 groups with 8 rats in each group, including normal control group, model group, febuxostat group and allopurinol group. Rats in model group and experimental group were given potassium oxazinate and yeast extract by gavage to establish a rat model of hyperuricemia. Febuxostat （10 mg·kg-1·d-1） and allopurinol （50 mg·kg-1·d-1） were given by gavage respectively to febuxostat group and allopurinol group for 6 weeks. After drug treatment, the levels of serum uric acid, interleukin-6 （IL-6）, nitric oxide （NO）, and endothelin-1 （ET-1） were measured by ELISA. The changes of Tregs and Th17 cells were measured by flow cytometry. HE and Masson staining were used to observe pathological changes of aortic endothelium tissues. Results: After febuxostat treatment, the levels of serum uric acid, IL-6 were significantly decreased （P<0.05）. The effects of febuxostat on reducing serum uric acid, IL-6 was better than that of allopurinol （P<0.05）. The levels of serum NO in the febuxostat group were higher than that in the model group （P<0.05）, while the levels of ET-1 were lower than that in the model group （P<0.05）. After febuxostat treatment, the percentages of Th17 cells were decreased while the ratios of Treg/Th17 were increased （P<0.05）. Histopathologic results showed that febuxostat could improve the swelling of endothelial cells and reduce the proliferation of fibrous tissue and deposition of collagen fibers. Conclusion: Febuxostat can improve vascular endothelial dysfunction in hyperuricemia rats, which may be related to its correction of Treg/Th17 imbalance.
陈凝, 孙红, 郑炜平, 等.. 非布司他对高尿酸血症大鼠Treg/Th17平衡及血管内皮功能的影响[J]. 江苏大学学报:医学版, 2022, 32(03): 236-240.
CHEN Ning, SUN Hong, ZHENG Weiping1, et al.. Effects of febuxostat on Treg/Th17 balance and vascular endothelialfunction in rats with hyperuricemia. Journal of Jiangsu University(Medicine Edition), 2022, 32(03): 236-240.
