Abstract:bjective: To investigate the effect of galangin on uric acid in acute hyperuricemia in mice after galangin was separated and purified from Rhizoma Alpiniae Officinarum. Methods: Galangin was extracted and purified from Rhizoma Alpiniae Officinarum using silica gel column chromatography alongside high performance liquid chromatography(HPLC) and thinlayer chromatography(TLC) were applied for qualitative and quantitative analysis of galangin. The acute hyperuricemia model of mouse was induced by hypoxanthine and potassium oxonate, all 70 Kunming mice were equally divided into seven groups: normal control group, positive control group (received intragastric administration of allopurinol), solvent control group (received intragastric administration of carboxymethyl celluose sodium), model group and galangin with high, moderate and low dose groups. Afterwards the level of uric acid and xanthine oxidase (XOD) activity in liver were detected. Results: The purity of the isolated galangin was 94.14%. The uric acid level of the normal group\[(62.76±27.73)μmol·L-1\] and positive control group \[(51.80±19.72)μmol·L-1\] was significantly lower than that of the model group \[(230.18±32.15)μmol·L-1 , P<0.01)\]. In addition, the uric acid level in mice of high and moderate dose galangin groups were lower than that of model group (P<0.05). Compared with the model group, the XOD in moderate dose galangin group was decreased significantly(P<0.05). Conclusion: Galangin was successfully isolated and purified from Rhizoma Alpiniae Officinarum. And it could reduce serum uric acid level in hyperuricemic mice, which might be attributed via the inhibition of xanthine oxidase activity.
收稿日期: 2017-04-17
基金资助:
国家自然科学基金资助项目(81373371,30973677)
通讯作者:
余江南(通讯作者),教授,博士生导师,E-mail:yjn@ujs.edu.cn
作者简介: 濮尊琴(1992—),女,硕士研究生
引用本文:
濮尊琴, 王启龙, 徐希明, 余江南. 高良姜素的分离纯化及降尿酸作用[J]. 江苏大学学报:医学版, 2017, 27(04): 338-343.
PU Zun-qin, WANG Qi-long, XU Xi-ming, YU Jiang-nan. Separation, purification of galangin and its effect on reducing uric acid. Journal of Jiangsu University(Medicine Edition), 2017, 27(04): 338-343.
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