宿主整合因子对伤寒沙门菌动力的影响

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摘要目的: 探究宿主整合因子(integration host factor，IHF)对伤寒沙门菌动力的影响及其潜在机制。方法: 选用IHF两个亚基HimA和HimD的基因缺失株(△himApBAD、△himD-pBAD），并构建相应回补株(himA-C、himD-C)，进行细菌动力实验，观察himA和himD对伤寒沙门菌动力的影响。通过实时荧光定量PCR比较野生株(WT-pBAD)与△himA-pBAD、野生株与△himD-pBAD中鞭毛相关基因flhD、fliA和fljB等mRNA表达差异，分析IHF对鞭毛基因的调控作用。结果：与WT-pBAD相比，△himA-pBAD和△himDpBAD动力均明显减弱(P均<0.05)，himA-C、himD-C动力则基本一致。与WTpBAD相比，△himApBAD、△himD-pBAD中鞭毛相关基因flhD、fliA和fljB的mRNA表达均显著降低(P<0.01或<0.05)。结论： IHF可能通过正向调节鞭毛相关基因flhD、fliA和fljB等转录，增强伤寒沙门菌的动力。

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	徐国新1
	朱晓珏1
	王芳2
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关键词 ：伤寒沙门菌, 宿主整合因子, 动力, 鞭毛基因

Abstract：Objective: To investigate the effect of integration host factor (IHF) on the motility of Salmonella enterica serovar Typhi and its potential mechanism. Methods: △himA-pBAD and △himD-pBAD, the mutations of the two subunits of IHF, were selected to construct relative gene complementary strains (himAC and himD-C). Motility assays were used to evaluate the effects of himA and himD on the motility of S. Typhi. Furthermore, quantitative realtime PCR was applied to analyze the mRNA levels of flhD, fliA and fljB among WT-pBAD, △himA-pBAD and △himD-pBAD. Results: Compared with WTpBAD, the motilities of both △himA-pBAD and △himD-pBAD were significantly weaker(P<0.05), while himA-C and himD-C did not cause any change in the motilities. In addition, the mRNA levels of flhD, fliA and fljB in △himA-pBAD and △himD-pBAD were also greatly lower than those in WTpBAD (P<0.01 or <0.05). Conclusion: IHF may promote the motility of S. Typhi through activating the transcriptions of flhD, fliA and fljB.

收稿日期: 2020-02-16

基金资助:张家港市科技支撑计划项目(ZKS1718，ZKS1824)；张家港青年科技项目(ZJGQNKJ201907)

作者简介: 徐国新(1979—)，男，江苏张家港人，副主任技师，主要从事病原微生物分子诊断研究。

引用本文:

徐国新1，朱晓珏1，王芳2，陈龙1. 宿主整合因子对伤寒沙门菌动力的影响[J]. 江苏大学学报:医学版, 2021, 31(01): 61-63. XU Guoxin1, ZHU Xiaojue1, WANG Fang2, CHEN Long1.

Effect of integration host factor on the motility of Salmonella enterica serovar Typhi

. Journal of Jiangsu University(Medicine Edition), 2021, 31(01): 61-63.

链接本文:

http://zzs.ujs.edu.cn/xbyxb/CN/ 或 http://zzs.ujs.edu.cn/xbyxb/CN/Y2021/V31/I01/61

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