聚乳酸羟基乙酸共聚物载脑源性神经生长因子纳米粒的构建及生物学活性评价

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摘要目的: 制备脑源性神经营养因子（brain-derived neurotrophic factor,BDNF）聚乳酸羟基乙酸共聚物\[poly(lactic-co-acid-glycolicacid), PLGA\]纳米粒(BDNFPLGA纳米粒) 并评价其理化性质及生物活性。方法: 通过复乳化法制备纳米粒，采用正交设计优化其制备工艺，以纳米粒的包封率、载药量为指标确定最佳工艺。在优化的条件下制备BDNF-PLGA纳米粒并对其进行表征。通过Alarma Blue增殖实验评价BDNF-PLGA纳米粒对外胚层间充质干细胞(ectomesenchymal stem cells, EMSCs)的毒性。结果：制备BDNF-PLGA纳米粒的优化条件为初乳和外水相的体积比为1 ∶15，投药量与PLGA用量质量比为6 ∶100，聚乙烯醇溶液质量浓度为10 g/L，超声时间为5 min。经优化后制备的BDNF-PLGA纳米粒粒径（205±0.18）nm，包封率（79.65±2.14）%，体外释放度曲线表现具有缓释效果；细胞增殖实验显示，BDNF-PLGA纳米粒可以促进EMSCs的增殖。结论：复乳法制备的BDNF-PLGA纳米粒包封率高，可缓释药物且具有良好的生物相容性。

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	李洋
	余江南
	徐希明

关键词 ： PLGA纳米粒, 正交设计, 脑源性神经营养因子, 缓释, 外胚层间充质干细胞

Abstract：Objective: To prepare the brain-derived neurotrophic factor (BDNF) poly (lactic-coglycolic acid) copolymer (PLGA) nanoparticles (BDNFPLGANPs) and to investigate its properties and biological activities. Methods: PLGA-NPs were prepared by double emulsion-solvent evaporation method.The formulation process was optimized by orthogonal test according to entrapment efficiency(EE) and drug loading. BDNF-PLGANPs were prepared and characterized under optimized conditions. The cell biocompatibility of BDNF-PLGA-NPs was evaluated by Alarma Blue proliferation assay. Results: The optimal conditions for preparation of BDNF-PLGA-NPs included a volume ratio of primary emulsion to external water phase of 1 ∶15， the mass ratio of dosage to PLGA dosage of 6 ∶100, a concentration of polyvinyl alcohol of 10 g/L and an ultrasonic time of 5 minutes. The BDNF-PLGANPs obtained had a diameter of (205±0.18) nm and an EE of (79.65 ± 2.14)%. The release curve shows that the BDNF-PLGA-NPs have a sustained release effect; BDNFPLGANPs can promote the proliferation of ectomesenchymal stem cells in vitro. Conclusion: BDNF-PLGA-NPs prepared by double solvent volatilization method under optimized conditions have high encapsulation rate, good sustained release effect and biological activity.

收稿日期: 2020-04-10

基金资助:国家自然科学基金资助项目(81773695)

通讯作者: 徐希明（通讯作者），教授，博士生导师，E-mail：xmxu@ujs.edu.cn

作者简介: 李洋（1996—），女，硕士研究生

引用本文:

李洋，余江南，徐希明. 聚乳酸羟基乙酸共聚物载脑源性神经生长因子纳米粒的构建及生物学活性评价[J]. 江苏大学学报:医学版, 2021, 31(01): 28-32，37. LI Yang， YU Jiangnan， XU Ximing.

Preparation and bioactivity evaluation of brain-derived neurotrophic factor-loaded poly (lactic-co-glycolic acid) nanoparticles

. Journal of Jiangsu University(Medicine Edition), 2021, 31(01): 28-32，37.

链接本文:

http://zzs.ujs.edu.cn/xbyxb/CN/ 或 http://zzs.ujs.edu.cn/xbyxb/CN/Y2021/V31/I01/28

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