Abstract:Objective: To construct and evaluate glucosamine sulfate liposome (GAS-Lips), compare the treatment of glucosamine sulfate (GAS) and GAS-Lips on osteoarthritis from anterior cruciate ligament transection (ACLT). Methods: The GASLips were constructed by film dispersion method and evaluated for their particle size, morphology, drug loading content and retention in vivo. Twentyfour SD rats were randomly divided into sham operation group, ACLT+ untreated group, ACLT+GAS group, and ACLT+GAS-Lips group. In vivo efficacy experiments were carried out by injecting normal saline, GAS and GAS-Lips into the joint cavity, and their therapeutic effects on osteoarthritis were observed. Results: The result of characterization experiment showed that an average particle size is (255.02±3.64) nm, with a spherical shape. The pharmaceutics evaluation showed that the GAS-Lips had a entrapment efficiency of (78.62±1.01)%, and drug loading content of (9.90±0.75)%, and had a good sustained-release effect. In vivo retention experiments showed that the Cy3-Lips group was significantly longer in the joint cavity when compared with the control group (P<0.01). In vivo efficacy experiments showed that GAS-Lips were more effective in alleviating joint surface damage than free GAS, inhibited the expression of inflammatory factors (both P<0.01), and had no significant effect on body mass in rats. Conclusion: This study successfully constructed and evaluated a GAS-Lips, improved the retention time and treatment effect of GAS in osteoarthritis, and provided the research basis for its development into osteoarthritis nano-preparation.
[Key words]glucosamine sulfate; liposome; osteoarthritis; joint cavity injection; nanopreparation; inflammatory factors
纪帅帅, 闫洁, 王子吟, 等.. 硫酸氨基葡萄糖脂质体的制备及其对骨关节炎的治疗作用[J]. 江苏大学学报:医学版, 2022, 32(03): 266-271.
JI Shuaishuai, YAN Jie, WANG Ziyin, et al.. Preparation of sulfate glucosamine liposome and its therapeutic effect on osteoarthritis. Journal of Jiangsu University(Medicine Edition), 2022, 32(03): 266-271.