食管癌细胞来源的外泌体促进人脐静脉血管内皮细胞血管生成

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摘要目的: 探讨食管癌细胞EC109分泌的外泌体（EC109 derived exosomes, EC109-ex）对人脐静脉血管内皮细胞（HUVECs）成管能力的促进作用。方法：培养食管癌细胞EC109至对数生长期，收集细胞上清液，梯度超速离心法提取外泌体，并采用蛋白质印迹法检测外泌体的表面特异性分子CD9、CD63，透射电镜观察外泌体的结构和粒径。荧光染料CM-DiR标记EC109-ex并与HUVECs共培养，荧光显微镜观察HUVECs摄取EC109-ex。分别加入PBS（对照组）、EC109ex（5 μg/mL和10 μg/mL）与HUVECs共培养，通过Transwell和细胞成管实验观察EC109-ex对于HUVECs的侵袭和成管能力的促进作用，通过蛋白质印迹法检测HUVECs血管特异性标志物CD31、CD34和血管内皮生长因子（VEGF）的表达。结果：EC109-ex能够定位于HUVECs的胞质。与对照组相比，与EC109ex共培养后，HUVECs的侵袭（P<0.01）和成管能力（P<0.01）均显著提高，血管特异性标志物CD31、CD34和VEGF的表达也显著增强（P<0.01）。结论：食管癌细胞可通过旁分泌外泌体途径促进肿瘤血管的生成。

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	施晋升
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	张秀梅

关键词 ：食管癌, 外泌体, 人脐静脉血管内皮细胞, 血管生成

Abstract：Objective: To explore the promotion effects of exosome from human esophageal cancer cells EC109 （EC109-ex） on the tube formation of human umbilical vein endothelial cells （HUVECs）. Methods: EC109ex was isolated from the supernatant of cultured EC109. EC109ex was characterized in terms of surface markers CD9 and CD63 by Western blotting and morphology by transmission electron microscopes （TEM）. HUVECs were treated with EC109ex （5 μg/mL and 10 μg/mL） or PBS （control group）, respectively. Then invasion and tube formation of HUVECs were examined using Transwell and Matrigel assay. Expression of vascular specific marker CD31, CD34 and VEGF were examined by Western blotting．Results: EC109ex located in the cytoplasm of HUVECs. Compared with control group，EC109-ex increased the migration efficiency (P<0.01) and number of tube formation of HUVECs （P<0.01）．Furthermore, EC109ex treatment increased expression of vascular specic markers of CD31, CD34 and VEGF in HUVECs（P<0.01）. Conclusion: Esophageal cancer cells could promote tumor angiogenesis through exosome mediated paracrine action.

收稿日期: 2020-12-08

基金资助:泰州市科技支撑计划社会发展项目（2018-35）；南京医科大学康达学院科研发展基金资助项目（KD2018KYJJYB038）

作者简介: 施晋升（1982—），男，江苏如东人，主治医师，硕士，主要从事食管癌和肺癌的治疗研究。

引用本文:

施晋升, 王茂松, 李本忠, 张秀梅. 食管癌细胞来源的外泌体促进人脐静脉血管内皮细胞血管生成[J]. 江苏大学学报:医学版, 2021, 31(05): 412-416，420. SHI Jinsheng, WANG Maosong, LI Benzhong, ZHANG Xiumei.

Human esophageal cancer cells derived exosomes enhance the angiogenesis of human umbilical vein endothelial cells

. Journal of Jiangsu University(Medicine Edition), 2021, 31(05): 412-416，420.

链接本文:

http://zzs.ujs.edu.cn/xbyxb/CN/ 或 http://zzs.ujs.edu.cn/xbyxb/CN/Y2021/V31/I05/412

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