The clinical evaluation of antinuclear antibody patterns and titer using automated indirect immunofluorescence interpretation system based on EUROPattern Suit technology" />
The clinical evaluation of antinuclear antibody patterns and titer using automated indirect immunofluorescence interpretation system based on EUROPattern Suit technology
XU Hong-xing, LIU Yi, ZHU Qin-fang, XU Wei-dong
(Department of Clinical Laboratory, the Affiliated Suzhou Municipal Hospital of Nanjing Medical University, Suzhou Jiangsu 215002,China)
Abstract:Objective: To evaluate the clinical application of antinuclear antibody (ANA) patterns and titer using the automated indirect immunofluorescence interpretation system based on EUROPattern Suit technology. Methods: A total of 202 serum samples were selected from Suzhou Municipal Hospital, including 177 patients with autoimmune diseases and 25 healthy controls. The antinuclear antibodies of two groups were determined by Sprinter XL automatic immunofluorescence analyzer, and then the ANA patterns and titer were evaluated using the EUROPattern Suit automated immunofluorescence interpretation system and traditional visual interpretation method. Results: ① The positive rates of two methods were respectively 85.6% and 86.1% , and there were no significant difference between two methods(P=0.886); The performance verification indicators, such as sensitivity, specificity, accuracy, positive predictive value and negative predictive value were compared, and none of the differences were significantly (P>0.05). ② It was shown that there were 169 positive cases and 24 negative case at the same time detected in two methods, the overall coincidence rate was 95.5% and the Kappa value of the consistency checking was 0.816. ③ The overall concordance rate of ANA patterns distribution in two methods was 85.1%. The concordance rates of different fluorescence patterns were respectively nuclear dots (100%),centromeres (93.8%), homogeneous (91.7%), nucleolar (88.5%), cytoplasmic (84.6%), speckled and mixed patterns (80.0%). ④ The concordance rates of ANA titer of every pattern in two methods were higher , ranging from 81.8% to 100.0%, except for the mixed pattern. Conclusion: Compared with the traditional visual interpretation method, the EUROPattern Suit automated fluorescence interpretation system had a higher sensitivity, specificity and concordance rate in interpreting the ANA pattern and titer. This assay can meet clinical requirements.
The clinical evaluation of antinuclear antibody patterns and titer using automated indirect immunofluorescence interpretation system based on EUROPattern Suit technology
. Journal of Jiangsu University(Medicine Edition), 2020, 30(02): 165-168.
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