Effects of different polar extracts of Coreopsis tinctoria on proliferation of spleen-derived CD4+ T cells of mouse in vitro
CAO Feng-hua1，2, SHEN Hua1, MA Jie1, XU Hua-xi1, CHEN Gang3, LI Ning3, WANG Sheng-jun1
（1. School of Medicine, Jiangsu University, Zhenjiang Jiangsu 212013； 2. Zhenjiang Hospital of Chinese Traditional and Western Medicine, Zhenjiang Jiangsu 212004； 3. School of Traditional Chinese Medicine, Shenyang Pharmaceutical University，Shenyang Liaoning 110016，China）
Abstract：Objective: To observe the effects of different extracts of Coreopsis tinctoria on proliferation of spleenderived CD4+ T cells of mouse in vitro. Methods: Sequoia petroleum ether extract (X-1), ethyl acetate extract (X-2) , N-butanol extract (X-3) and okanin (X21) were obtained by extracting with 95% ethanol and different polar solvents. Spleen-derived CD4+ T cells of mouse were isolated by immunomagnetic beads method and placed in 96well plates at a cell density of 5×105 cells/well. Different concentrations of extracts and okanin were added to the basis of ConA, and MTT was used to detect the proliferation of cells after 48 h of co-culture. At the same time, cytotoxicity experiments were carried out, different concentrations of extracts and okanin were given without adding ConA. The survival rate of the cells was detected by MTT assay after 48 h of co-culture. Results: Ethyl acetate extract (X2) inhibited the proliferation of CD4+ T cells in a concentration-dependent manner significantly. The main component of X2, okanin, also showed the same effect, the cell proliferation rate was only (19.23±0.57)% at a concentration of 10 μmol/L, and it showed no significant toxicity to normal cells at the effective concentration. Conclusion: Ethyl acetate extract of Coreopsis tinctoria and its main component, okanin, can significantly inhibit ConAmediated proliferation of spleen-derived CD4+ T cells of mouse.
曹风华1，2， 沈花1， 马洁1， 许化溪1， 陈刚3， 李宁3， 王胜军1. 雪菊不同极性萃取物对小鼠脾脏CD4+T细胞体外增殖的影响[J]. 江苏大学学报:医学版, 2019, 29(05): 375-378,385.
CAO Feng-hua1，2, SHEN Hua1, MA Jie1, XU Hua-xi1, CHEN Gang3, LI Ning3, WANG Sheng-jun1.
Effects of different polar extracts of Coreopsis tinctoria on proliferation of spleen-derived CD4+ T cells of mouse in vitro. Journal of Jiangsu University(Medicine Edition), 2019, 29(05): 375-378,385.
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2019, Vol. 29 
