Abstract:Objective: The study was to investigate the expression and significance of TRIM25 and PTEN in myeloidderived suppressor cells (MDSCs) from tumor tissue of tumor-bearing (TB) mice. Methods: CT26 colon cancer tumor-bearing mice models were established. The expressions of TRIM25 and PTEN were detected in MDSCs derived from tumor tissue of TB mice and spleen of wild-type (WT) mice with qRTPCR method. Results: In CT26 cells bearing mice models, the expression level of TRIM25 in tumor tissue-derived MDSCs of TB mice was significantly higher than that from spleen of WT mice(P<0.001). Inversely, compared to WT mice, PTEN expression was obviously down-regulated in MDSCs isolated from tumor tissue of TB mice (P<0.001). Conclusion: TRIM25 expression level was up-regulated, while the expression of PTEN was suppressed in MDSCs from tumor tissue of TB mice. They both may participate in the regulation of MDSCs.
收稿日期: 2019-03-20
基金资助:
江苏省科技计划项目临床医学专项(BL2014065); 江苏大学大学生科研立项项目(15A332)
通讯作者:
王胜军(通讯作者),教授,博士生导师,E-mail:sjwjsu@aliyun.com
作者简介: 宋格(1991—),女,硕士研究生
引用本文:
宋格, 赵耀, 陆薇, 王胜军. 荷瘤小鼠MDSCs中TRIM25和PTEN的表达[J]. 江苏大学学报:医学版, 2019, 29(04): 333-338.
SONG Ge, ZHAO Yao, LU Wei, WANG Sheng-jun. Expression of TRIM25 and PTEN in MDSCs of tumor-bearing mice. Journal of Jiangsu University(Medicine Edition), 2019, 29(04): 333-338.
[1]Parker KH, Beury DW, OstrandRosenberg S. Myeloidderived suppressor cells: critical cells driving immune suppression in the tumor microenvironment\[J\]. Adv Cancer Res, 2015, 128: 95-139.[2]Kumar V, Patel S, Tcyganov E, et al. The nature of myeloidderived suppressor cells in the tumor microenvironment\[J\]. Trends Immunol, 2016, 37(3): 208-220.[3]Sumida K, Wakita D, Narita Y, et al. AntiIL6 receptor mAb eliminates myeloidderived suppressor cells and inhibits tumor growth by enhancing Tcell responses\[J\]. Eur J Immunol, 2012, 42(8): 2060-2072.[4]Zhang P, Elabd S, Hammer S, et al. TRIM25 has a dual function in the p53/Mdm2 circuit\[J\]. Oncogene, 2015, 34(46): 5729-5738.[5]Mao H, Du Y, Zhang Z, et al. Nitroxoline shows antimyeloma activity by targeting the TRIM25/p53 axle\[J\]. Anticancer Drugs, 2017, 28(4): 376-383.[6]Lin H, Jiang M, Liu L, et al. The long noncoding RNA Lnczc3h7a promotes a TRIM25mediated RIGI antiviral innate immune response\[J\]. Nat Immunol, 2019, 20(7):812-823.[7]Qin X, Qiu F, Zou Z. TRIM25 is associated with cisplatin resistance in nonsmallcell lung carcinoma A549 cell line via downregulation of 1433sigma\[J\]. Biochem Biophys Res Commun, 2017, 493(1): 568-572.[8]Wang Z, Tong D, Han C, et al. Blockade of miR3614 maturation by IGF2BP3 increases TRIM25 expression and promotes breast cancer cell proliferation\[J\]. EBioMedicine, 2019, 41: 357-369.[9]Takayama KI, Suzuki T, Tanaka T, et al. TRIM25 enhances cell growth and cell survival by modulating p53 signals via interaction with G3BP2 in prostate cancer\[J\]. Oncogene, 2018, 37(16): 2165-2180.[10]Qin Y, Cui H, Zhang H. Overexpression of TRIM25 in lung cancer regulates tumor cell progression\[J\]. Technol Cancer Res Treat, 2016, 15(5): 707-715.[11]Sun N, Xue Y, Dai T, et al. Tripartite motif containing 25 promotes proliferation and invasion of colorectal cancer cells through TGFbeta signaling\[J\]. Biosci Rep, 2017, 37(4).pii:BSR20170805.[12]Zhu Z, Wang Y, Zhang C, et al. TRIM25 blockade by RNA interference inhibited migration and invasion of gastric cancer cells through TGFbeta signaling\[J\]. Sci Rep, 2016, 6: 19070.[13]Walsh LA, Alvarez MJ, Sabio EY, et al. An integrated systems biology approach identifies TRIM25 as a key determinant of breast cancer metastasis\[J\]. Cell Rep, 2017, 20(7): 1623-1640.[14]MartinVicente M, Medrano LM, Resino S, et al. TRIM25 in the regulation of the antiviral innate immunity\[J\]. Front Immunol, 2017, 8: 1187.[15]Naderali E, Khaki AA, Rad JS, et al. Regulation and modulation of PTEN activity\[J\]. Mol Biol Rep, 2018, 45(6): 2869-2881.[16]Mei S, Xin J, Liu Y, et al. MicroRNA200c promotes suppressive potential of myeloidderived suppressor cells by modulating PTEN and FOG2 expression\[J\]. PLoS One, 2015, 10(8): e0135867.[17]Li L, Zhang J, Diao W, et al. MicroRNA155 and MicroRNA21 promote the expansion of functional myeloidderived suppressor cells\[J\]. J Immunol, 2014, 192(3): 1034-1043.[18]Liu Y, Lai L, Chen Q, et al. MicroRNA494 is required for the accumulation and functions of tumorexpanded myeloidderived suppressor cells via targeting of PTEN\[J\]. J Immunol, 2012, 188(11): 5500-5510.[19]Tcyganov E, Mastio J, Chen E, et al. Plasticity of myeloidderived suppressor cells in cancer\[J\]. Curr Opin Immunol, 2018, 51: 76-82.[20]Gabrilovich DI, Nagaraj S. Myeloidderived suppressor cells as regulators of the immune system\[J\]. Nat Rev Immunol, 2009, 9(3): 162-174.[21]Noman MZ, Desantis G, Janji B, et al. PDL1 is a novel direct target of HIF1alpha, and its blockade under hypoxia enhanced MDSCmediated T cell activation\[J\]. J Exp Med, 2014, 211(5): 781-790.[22]Yin Z, Li C, Wang J, et al. Myeloidderived suppressor cells: Roles in the tumor microenvironment and tumor radiotherapy\[J\]. Int J Cancer, 2019, 144(5): 933-946.[23]Garcia AJ, Ruscetti M, Arenzana TL, et al. Pten null prostate epithelium promotes localized myeloidderived suppressor cell expansion and immune suppression during tumor initiation and progression\[J\]. Mol Cell Biol, 2014, 34(11): 2017-2028.[24]Tarcic O, Pateras IS, Cooks T, et al. RNF20 links histone H2B ubiquitylation with inflammation and inflammationassociated cancer\[J\]. Cell Rep, 2016, 14(6): 1462-1476.[25]何远明.泛素连接酶TRIM25对PTEN/AKT信号通路的调节机制及其在非小细胞肺癌中的作用研究[D].苏州:苏州大学,2018.