Abstract：Objective： To investigate the effect of atractylodin on proliferation of human colon cancer LS174T cells. Methods： LS174T cells were divided into six groups, including blank control group, absolute ethyl alcohol control group, and atractylodin-treated groups(10, 20, 40, 80 mg/L). The changes of cell morphology were observed with optical microscope; inhibition of cell proliferation by atractylodin was measured with MTT assay; cell cycle analysis was analyzed with flow cytometry; the cytokine expression was measured with liquichip method. Results： Atractylodin induced LS174T cell growth inhibtion in a time- and concentration-dependent manner. G0/G1phase ratio of LS174T cells treated by atractylodin was increased. Atractylodin inhibited the expression of IL-6 and IL-7 in LS174T cells. Conclusion： Atractylodin could inhibit the proliferation of LS174T cells in a dose- and time-dependent manner, which may be caused by blocking the cell cycle progression and the expression of associated cytokines.
［Key words］atractylodin; colon cancer; proliferation
2016, Vol. 26 
