Abstract：Objective: To explore the mutation of K-ras in biopsy samples of fiberoptic bronchoscopy(FB) in patients with lung cancer and evaluate its clinical value of lung cancer.Method: PCR-single strand conformational polymorphism(PCR-SSCP) methods and silver staining technique was used to examine the mutation at K-ras for biopsy samples of FB in 52 patients with lung cancer and 9 patients with benign pulmonary disease. Results: The mutation rate of K-ras gene was 23.1%(12/52)and 0%(0/9) in lung biopsy samples in patient with lung cancer and benign pulmonary disease, respectively. The mutation of K-ras was obvious in adenocarcinoma cell carcinomas, but not significantly associated with patients age, sex, smoking history, cell differentiation or clinical stage of lung cancer.Conclusions: K-ras gene mutation may play important role in genesis and progression of lung cancer and may be an early event of lung cancer.Mutation of K-ras gene in biopsy samples of FB may be used to diagnose lung cancer. ［Key words］lung cancer; fiberoptic bronchoscopy; K-ras gene; mutation
作者简介: 陈萍（1972—），女，江苏镇江人，副主任医师，硕士，主要从事呼吸内科疾病的研究。
引用本文:
陈萍, 李坚, 朱文静, 杭建明. 肺癌患者纤维支气管镜活检标本K-ras基因突变的检测及其临床意义[J]. 江苏大学学报:医学版, 2013, 23(6): 509-.
CHEN Ping, Li-Jian, Zhu-Wen-Jing, Hang-Jian-Ming. Mutation of Kras gene in lung cancer tissues obtained by fiberoptic bronchoscopy and its clinical value. Journal of Jiangsu University(Medicine Edition), 2013, 23(6): 509-.
2013, Vol. 23 
