Effect of microRNA-21 transfection on PDCD4 expression andcell proliferation in cervical cancer cells
DAI Gui-hong1, LI Guo-li2, QIAN Hua1, YUAN Dong-lan3, JIAO Xia1, MEI Jia4, ZHU Xiao-wei1, YU Hong1
(1.Department of Pathology, Taizhou People′s Hospital, Taizhou Jiangsu 225000; 2. School of Medical College, Yangzhou University, Yangzhou Jiangsu 225000; 3. Department of Gynaecology, Taizhou People′s Hospital, Taizhou Jiangsu 225300; 4. Department of Pathology, PLA No.81 Hospital, Nanjing Jiangsu 210001, China)
Abstract：[Abstract] Objective: To investigate the effect of miR-21 transfection on the expression of programmed cell death 4(PDCD4) and cell proliferation in cervical cancer cell line HeLa cells, and to provide experimental foundation for miR-21 gene therapy of cervical cancer. Methods: Lipid-mediated transfection was used to transfect pre-miR-21，pre-miR-21 negative control and anti-miR-21,anti-miR-21 negative control into HeLa cervical cancer cells. The miR-21 expression was measured by quantitative real-time PCR(qRT-PCR). The cellular growth activity was determined by MTT assay. The protein expression of PDCD4 was measured by western blotting. Detection of the target sites of miR-21 was analyzed by luciferase report gene assays. Results: In pre-miR-21 transfected cells, the cellular growth activity increased, and the expression of PDCD4 protein decreased. Conversely, in anti-miR-21 transfected cells, the cellular growth activity decreased, and the expression of PDCD4 protein increased. pMIR-Luc-PDCD4-3′UTR co-transfected with pre-miR-21, luciferase activity decreased; while it co-transfected with anti-miR-21, luciferase activity increased. Conclusion: PDCD4 gene was the target gene of miR-21, which suggested the potential possibility of miR-21 as a target gene for cervical cancer therapy.
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2013, Vol. 23 
