1.Research Division of Clinical Pharmacology, the First Affiliated Hospital of Nanjing Medical University, Nanjing Jiangsu 210029; 2.Unit of Viral Immunology, Institute Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai 200025; 3.Department of General Surgery, the First Affiliated Hospital of Nanjing Medical University, Nanjing Jiangsu 210029, China
Abstract：Objective: To establish the technique for generation or isolation of human primary mast cells,and for further studying their roles in regulating host immunity and elucidating immunopathogenesis. Methods: CD34+hemopoietic stem cells were cultured in presence of stem cell factor, IL6, and IL3 to generate primary mast cells.Mast cells allocated in human intestinal tissues were isolated through digestion, density gradient centrifugation and purification with specific antibodiescoated magnetic beads. Cell phenotype was monitored by immuostaining of surface markers of CD117 and FcεRⅠ and intracellular tryptase, or cells were identified with Toluidine blue staining. Results: Both of the CD34+cellderived and intestinal mast cells expressed CD117 and FcεRⅠ， the specific markers for mast cells, and contained intracellular tryptase. Conclusion: Both methods could provide sufficient and purified primary mast cells for further study in regulating host immune response and defence against pathogenic microorganism.
翟荣荣1, 2, 蒋金凤2, 张济3, 丁永斌3, 王建华2, 魏继福1. 人原代肥大细胞体外诱导和组织分离方法的建立[J]. 江苏大学学报:医学版, 2013, 23(3): 224-.
DI Rong-Rong-1, 2 , JIANG Jin-Feng-2, ZHANG Ji-3, DING Yong-Bin-3, WANG Jian-Hua-2, WEI Ji-Fu-1. Establishment of techniques for generation in vitro and isolation ex vivo for human primary mast cells. Journal of Jiangsu University(Medicine Edition), 2013, 23(3): 224-.
2013, Vol. 23 
