(1.School of Medical Science and Laboratory Medicine, Jiangsu University, Zhenjiang Jiangsu 212013; 2.Department of Laboratory Medicine, the People′s Hospital of Guangxi Zhuang Autonomous Region，Nanning Guangxi 530022, China)
Abstract：Objective: To investigate the effect of Epstein-Barr virus-encoded BARF1 gene on the nuclear translocation of NF-κB and bcl-2 expression in human nasopharyngeal carcinoma cells. Methods: Fluorescence antibody staining and Western blot were separately performed to analyze the nuclear translocation of NFκB and bcl-2 expression in BARF1-stably-expressing human nasopharyngeal carcinoma cell line CNE1 (CEN1-BARF1) under the control of pSG5 vector transfected one (CEN1-SG). Results: Compared with the control, the nuclear translocation of NF-κB was significantly induced, and bcl-2 expression was significantly increased in CEN1-BARF1. And both were blocked by the specific inhibitor of NF-κB Bay11-7082. Conclusion: BARF1 gene upregulated bcl-2 expression through activating NF-κB signaling pathway in human nasopharyngeal carcinoma cells.
[1]Takada K. Role of EBER and BARF1 in nasopharyngeal carcinoma (NPC) tumorigenesis ［J］. Semin Cancer Biol, 2012,22(2):162-165.[2]Raab-Traub N. Novel mechanisms of EBV-induced oncogenesis［J］. Curr Opin Virol, 2012,2(4):453-458.[3]Seto E, Yang L, Middeldorp J, et al. Epstein-Barr virus (EBV)encoded BARF1 gene is expressed in nasopharyngeal carcinoma and EBV-associated gastric carcinoma tissues in the absence of lytic gene expression［J］. J Med Virol, 2005,76(1):82-88.［4］李友琼, 张雪怡, 曾健, 等. EB病毒BARF1基因表达对胃癌细胞株SGC7910生物学行为的影响［J］. 江苏大学学报：医学版, 2009,19(03):214-219.［5］Jiang R, Cabras G, Sheng W, et al. Synergism of BARF1 with Ras induces malignant transformation in primary primate epithelial cells and human nasopharyngeal epithelial cells［J］. Neoplasia, 2009,11(9):964-973.［6］Wang Q, Tsao SW, Ooka T, et al. Anti-apoptotic role of BARF1 in gastric cancer cells［J］. Cancer Lett, 2006,238(1):90-103.［7］Hayden MS, Ghosh S. Shared principles in NF-kappaB signaling［J］. Cell, 2008,132(3):344-362.［8］Escarcega RO, Fuentes-Alexandro S, Garcia-Carrasco M, et al. The transcription factor nuclear factor-kappa B and cancer［J］. Clin Oncol (R Coll Radiol), 2007,19(2):154-161.［9］Baldwin AS. Regulation of cell death and autophagy by IKK and NF-kappaB: critical mechanisms in immune function and cancer ［J］. Immunol Rev, 2012,246(1):327-345.［10］Perkins ND. The diverse and complex roles of NF-kappaB subunits in cancer ［J］. Nat Rev Cancer, 2012,12(2):121-132.［11］Fiorini S, Ooka T. Secretion of Epstein-Barr virus-encoded BARF1 oncoprotein from latently infected B cells ［J］. Virol J, 2008,5:70.［12］Ooka T. BARF-1 gene as an EBV encoded oncogene ［J］. EpsteinBarr Virus Res, 2001,8(1):177-182.［13］Sheng W, Decaussin G, Sumner S, et al. Nterminal domain of BARF1 gene encoded by Epstein-Barr virus is essential for malignant transformation of rodent fibroblasts and activation of BCL2 ［J］. Oncogene, 2001,20(10):1176-1185.［14］Fu Q,He C,Mao ZR.Epstein-Barr virus interactions with the Bcl-2 protein family and apoptosis in human tumor cells ［J］.J Zhejiang Univ Sci B, 2013,14(1):8-24.［15］Suh J, Rabson AB. NF-kappaB activation in human prostate cancer: important mediator or epiphenomenon? ［J］. J Cell Biochem, 2004,91(1):100-117.［16］曾健,李友琼,王文红, 等. EB病毒BARF1蛋白诱导胃癌细胞c-met和Bcl-2基因过表达 ［J］. 江苏医药, 2010,36(08):913-917.
2013, Vol. 23 
