Abstract：Objective: To build temozolomide resistant glioma cell line in vitro, and to investigate its biological characteristics. Methods: By using pulse exposure to induce and build resistant to temozolomide(TMZ) human glioma U251 cell lines (named U251／TMZ); MTT was applied to detect the sensitivity of the parental cells (U251) and U251／TMZ to TMZ; drew proliferation curves of both cells and their growing conditions were compared; immunofluorescence technique was applied to detect CD133 expression; western blot assay was applied to detect the ABCG2 expression of both cells. Results: Temozolomide resistant glioma cell line had been successfully constructed and the resistance index of U251／TMZ was 8.1 times (t=-63.28,P=0.00) as high as parental cells; the proliferation U251／TMZ was slower than that of parental cells; by applying immunofluorescence assay to detect CD133 expression, there was a significant increase in U251／TMZ compared with parental cells(t=45.35,P=0000); by applying western blot assay to detect ABCG2 expression, it was found that U251／TMZ was significantly higher than pro-generation(t=6.19,P=0.00). Conclusion: The U251／TMZ developed with the method of pulse exposure displayed a certain amount of resistance, and possessed obvious biological characteristics of glioma stem cells.
向定朝1, 王存祖2, 陆晓峰1, 欧阳琦3. 大剂量冲击法建立胶质瘤耐替莫唑胺细胞株及生物学鉴定[J]. 江苏大学学报:医学版, 2013, 23(1): 22-25.
XIANG Ding-chao1, WANG Cun-zu2, LU Xiao-feng1, OUYANG Qi3.
Development and characterization of temozolomide resistant glioma cell line by pulse exposure. Journal of Jiangsu University(Medicine Edition), 2013, 23(1): 22-25.
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2013, Vol. 23 
