Abstract：Objective: To investigate the effect of N-myc downstream regulated gene 1(NDRG1) on the proliferation in human cervical cancer cell line SiHa, with a focus on the expression of cyclooxygenase-2(COX-2) and vascular endothelial growth factor(VEGF). Methods： pcDNA3.0-NDRG1 and pcDNA3.0 were transfected into SiHa cells through lipofectamine and positive clones were screened by G418. RT-PCR and Western blot were employed to identify mRNA and protein expression of NDRG1 in SiHa cells, respectively. The expression of COX-2 and VEGF in positive clones was detected by Western blot. Cell viability was assessed by MTT method. Results： Positive clone N-12 with NDRG1 overexpression was successfully established. Compared with non-transfected control group, the expression of COX-2 and VEGF was decreased significantly, and the proliferation level of N-12 was also decreased significantly. Conclusion： It is possible that NDRG1 could inhibit the development of cervical cancer by downregulating the expression of COX-2 and VEGF and the proliferation in SiHa cells.
钱华1， 王华1*， 袁冬兰1， 崔永安2， 林梅2， 肖蔚3， 焦霞3， 于鸿3. 转染NDRG1基因对宫颈癌细胞COX-2、VEGF表达及增殖的影响[J]. 江苏大学学报:医学版, 2012, 22(6): 472-475.
QIAN Hua1, WANG Hua1*, YUAN Dong-lan1, CUI Yong-an2, LIN Mei2, XIAO Wei3, JIAO Xia3， YU Hong3. Effect of gene NDRG1 transfection on the expression of COX-2 and VEGF and proliferation in human cervical cancer cells. Journal of Jiangsu University(Medicine Edition), 2012, 22(6): 472-475.
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2012, Vol. 22 
