Impact of natural salt powder inhalation treatment in asthmatic mice to the pathological changes of lung and blood serum levels of IL-4,IL-5 and IL-13
FAN Li-juan1，XU Hua-jun2, WU Zhan-ao3， DUAN Li-rong4
(1.Department of Respiratory Medicine, the No.359 Hospital of Chinese PLA, Zhenjiang Jiangsu 212001； 2.Zhenjiang Salt Chemical Company,Zhenjiang Jiangsu 212001； 3.Department of Stomatology, the No.359 Hospital of Chinese PLA, Zhenjiang Jiangsu 212001； 4.School of Medical Science and Laboratory Medicine,Jiangsu University, Zhenjiang Jiangsu 212013，China)
Abstract：Objective: To investigate the pathological changes of lung and the influence to serum IL-4, IL-5 and IL-13 levels in asthmatic mice treated by salt powder inhalation. Methods: Forty Balb/c female mice were divided randomly into control group, model group, therapy group A, therapy group B with each group of 10 only. On day 0 and 7, the mice of model group, therapy group A and therapy group B were injected intraperitoneal with liquid sensitized(ovalbumin), while the mice of control group with saline. On day 14, the mice inhaled the aerosol of 1% ovalbumin to induce asthma for the first time. On day 15, therapy group A and therapy group B were treated with salt powder inhalation for 30 min and 60 min every day, respectively. The control group and the model group were treated with air inhalation. On day 22, 23, 24, the asthma symptoms were induced with the same method continuously. On day 25, all mice were put into death. The lungs of mice were observed by pathological method. The levels of serum IL-4, IL-5, IL-13 were tested by ELISA. Results: Compared with control group, the lungs of model group mice had bronchial constriction, mucosal epithelial necrosis, inflammatory cells infiltration with eosinophils gathered. Compared with model group, the therapy group A and therapy group B improved markedly. The levels of serum IL-4, IL-5 of model group were higher than those of the control group obviously(P＜0.01). And the level of IL-13 of model group was high too(P＜0.05). The levels of IL-4, IL-5 and IL-13 of therapy group A and therapy group B were lower than those of model group significantly(P＜0.01).Conclusion: Inhaling natural salt powder could alleviate airway inflammation of bronchial asthma mice, and reduce the levels of related cytokines IL-4, IL-5 and IL-13 in serum.
范利娟, 许华君, 吴占敖, 端礼荣. 矿盐粉粒吸入疗法对哮喘小鼠肺组织病理形态及血清IL-4、IL-5、IL-13水平的影响[J]. 江苏大学学报:医学版, 2012, 22(5): 374-377.
FAN Li-Juan, Xu-Hua-Jun, WU Zhan-Ao, Duan-Li-Rong. Impact of natural salt powder inhalation treatment in asthmatic mice to the pathological changes of lung and blood serum levels of IL-4,IL-5 and IL-13. Journal of Jiangsu University(Medicine Edition), 2012, 22(5): 374-377.
2012, Vol. 22 
