Abstract：Objective: To investigate the histological and molecule level expression and distribution of VEGF，Flt-1，Flk-1 in the pulp of young and mature permanent teeth and explore the role of VEGF，Flt-1，Flk-1 in the permanent root development. Methods: Young and mature permanent teeth were collected for orthodontic reasons，each group included 15 teeth. The expression and localization of VEGF，Flt-1，Flk-1 were studied with immunohistochemistry. VEGF，Flt-1，Flk-1 mRNA levels of young and mature permanent teeth dental pulp were assayed by RT-PCR. Results: Immunohistochemical staining detected the expression of VEGF and Flk-1，they were more positive in young permanent than the mature（P <0.05）; while Flt-1 was more positive in the mature; RT-PCR demonstrated VEGF，Flt-1，Flk-1 mRNA expression in young and mature permanent teeth pulp tissue with 145 bp，169 bp and 162 bp transcript respectively，and relative content of VEGF，Flt-1，Flk-1 in young permanent teeth were 1.7，0.6 and 1.5 times in mature ones. Conclusion: The expression of VEGF，Flt-1，Flk-1 in the pulp of young and mature permanent teeth showed different characteristics，which suggests that the reparative dentine formation of permanent teeth can be induced by regulating the upper genes at different development stages.
［1］ Ferrara N. VEGF and the quest for tumour angiogenesis factors［J］. Nat Rev Cancer，2002，2（10）:795-803. ［2］ Pimenta FJ， S AR， Gomez RS. Lymphangiogenesis in human dental pulp［J］. Int Endod J，2003，36（12）: 853-856. ［3］ Ferrara N. Role of vascular endothelial growth factor in regulation of physiological angiogenesis［J］. Am J Physiol Cell Physiol，2001，280（6）:C1358-1366. ［4］ Grando Mattuella L，Westphalen Bento L，de Figueiredo JA，et al. Vascular endothelial growth factor and its relationship with the dental pulp［J］.J Endod，2007，33（5）: 524-530. ［5］ Haralabkis NB， Yiagtzis SC， Toutountzakis NM. Prema- ture or delayed exfoliation of deciduous teeth and root resorption and formation［J］. Angle Orthod， 1994， 64（2）: 151-157. ［6］关为群，王清妹.牙体脱钙法和劈牙取髓法观察牙髓组 织中VEGF表达的效果比较［J］.江苏大学学报:医学版，2010，20（5）:409-411. ［7］ Tjaderhane L， Salo T， Larjava H， et al. A novel organ culture method to study the function of human odontoblasts in vitro: gelatinase expression by odontoblasts is differen- tially regulated by TGF-betal［J］. J Dent Res，1998，77 （7）:1486-1496. ［8］ Roberts DM， Kearney JB， Johnson JH， et al. The vascular endothelial growth factor（VEGF） receptor Flt-1 （VEGFR-1） modulates Flk-1（VEGFR-2） signaling during blood vessel formation［J］. Am J Pathol，2004，164 （5）:1531-1535. ［9］ Gille H，Kowalski J，Li B，et al. Analysis of biological effects and signaling properties of Flt-1（VEGFR-1） and KDR（VEGFR-2）. A reassessment using novel receptor specific vascular endothelial growth factor mutants［J］. J Biol Chem，2001，276（5）:3222-3230. ［10］倪效，燕敏.VEGF受体功能研究进展［J］.生命科学， 2008，20（1）:120-124. ［11］王双义，苏丽萍，曹建明，等.血管内皮生长因子在口腔颌面骨组织工程中的应用［J］.中国组织工程研究与临 床康复，2007，11（14）:2741-2744. ［12］ Zhang W，Yelick PC. Vital pulp therapy-current progress of dental pulp regeneration and revascularization［J］.Int J Dent， 2010， 16（1）: 1-10.
2012, Vol. 22 
