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Journal of Jiangsu University(Medicine Edition)

	2023 Vol.33 Issue.06 Published 2023-11-23

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		2023 Vol. 33 (06): 1- [Abstract] ( 11 ) [HTML 1KB] [ PDF 389KB] ( 606 )

	461	Inhibitory effect of acacetin on nonsmall cell lung cancer A549 cells and its mechanism

		Objective： To investigate the effect and molecular mechanisms of acacetin on migration, invasion and angiogenesis of non-small cell lung cancer (NSCLC) A549 cells. Methods: The effects of various concentrations of acacetin on A549 cell viability were detected by MTT assay and IC50 was calculated. The A549 cells were treated with various concentrations of acacetin, and the relative expression levels of vascular endothelial growth factor (VEGF), Ecadherin and matrix metalloproteinase-9 (MMP-9) were detected by Western blotting. And the ability of cell migration, invasion and angiogenesis was detected by scratch wound healing, Transwell, immunofluorescence and tubulogenesis tests, respectively. VEGF plasmid was overexpressed or knocked down in A549 cells, and the relative expression levels of VEGF, E-cadherin and MMP-9 were detected by Western blotting, and the ability of the migration, invasion and angiogenesis of the cells were further evaluated. Results: Compared with 0 μmol/L group, A549 cell viability in 10, 20, 30, 40 and 50 μmol/L acacetin groups was significantly decreased (P<0.01), and in a certain concentrationdependent manner, IC₅₀ was 34-49 μmol/L. Compared with 0 μmol/L group, the migration, invasion and angiogenesis of A549 cells in 10, 20 and 40 μmol/L acacetin groups were significantly decreased (all P<0.05), and the expression levels of VEGF and MMP-9 protein were significantly decreased (all P<0.01), while the expression level of E-cadherin protein was significantly increased (P<0.01). Compared with blank control or overexpression negative control group, the expression level of MMP-9 protein in A549 cells in overexpression group was significantly increased, while the expression level of E-cadherin protein was significantly decreased, and the ability of cell migration, invasion and angiogenesis was significantly enhanced (all P<0.05). Compared with blank control or knockdown negative control group, the expression level of MMP-9 protein in A549 cells in knockdown group was significantly decreased, while the expression level of E-cadherin protein was significantly increased, and the ability of cell migration, invasion and angiogenesis was significantly decreased (all P<0.05). Conclusion: Acacetin may inhibit migration, invasion and angiogenesis of NSCLC A549 cells by decreasing VEGF expression. ［Key words］nonsmall cell lung cancer; acacetin; angiogenesis; vascular endothelial growth factor (VEGF)
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		2023 Vol. 33 (06): 461-469 [Abstract] ( 18 ) [HTML 1KB] [ PDF 29851KB] ( 552 )

	470	Predictive value of systemic immune inflammatory index combined with prealbumin for postoperative pulmonary cancer infection after lung cancer surgery

		Objective： To explore the predictive value of systemic immune inflammation index (SII) combined with prealbumin for postoperative pulmonary infection after lung cancer surgery. Methods: A retrospective analysis was performed on 1 025 patients with lung cancer who underwent single lobectomy admitted to the Department of Thoracic Surgery, Affiliated Hospital of Xuzhou Medical University from January 2017 to December 2021. The patients were divided into infected group and non-infected group according to whether they were infected after surgery. The risk factors of postoperative lung infection were analyzed by univariate and multivariate Logistic regression. Receiver operating characteristic (ROC) curve was used to analyze the predictive efficacy of SII and prealbumin alone and in combination for postoperative lung infection. Results: Of the 1 025 patients with lung cancer, 108 cases (10.5%) developed pulmonary infection after surgery. Compared with the non-infected group, the infected group had higher ASA grade, TNM stage, leukocyte level and SII value, more patients with diabetes mellitus, longer operation time and maximum tumor diameter, and lower albumin and prealbumin levels (all P<0.05). Multivariate Logistic regression analysis showed that long operation time, combined diabetes mellitus, low prealbumin level and high SII value were independent risk factors for lung infection after lung cancer surgery (all P<0.05). ROC curve results showed that the area under curve (AUC) of SII was 0.705 (95%CI: 0.656-0.755), and the AUC of prealbumin was 0.626 (95%CI: 0.572-0.680); while the AUC of SII combined with prealbumin in predicting the occurrence of lung infection was 0.751, which was better than the value of single index in the diagnosis and prediction of postoperative lung infection after lung cancer surgery (P<0.05). Conclusion: SII and proalbumin could predict the occurrence of postoperative lung infection after lung cancer surgery, and the combination of SII and proalbumin has higher predictive value for the occurrence of lung infection. ［Key words］lung cancer; systemic immune inflammatory index; prealbumin; postoperative lung infection
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		2023 Vol. 33 (06): 470-474,485 [Abstract] ( 22 ) [HTML 1KB] [ PDF 4475KB] ( 495 )

	475	Expression and biological role of potassium voltage-gated channel subfamily G member 1 in lung cancer

		Objective： To investigate the expression level and clinical significance of potassium voltage-gated channel subfamily G member 1 (KCNG1) in human lung cancer tissues, and its role in the malignant biological behavior of lung cancer cells. Methods: The Cancer Genome Atlas (TCGA) database was consulted to explore the expression level of KCNG1 mRNA in human lung cancers, and the relationship between KCNG1 mRNA and clinicopathological features and prognosis of patients with lung cancer; nomogram model was constructed to predict the prognosis of lung cancer patients based on the KCNG1 mRNA expression; Gene Ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis were performed to explore the potential biological functions of KCNG1; gene set enrichment analysis (GSEA) was employed to analyze the differentially expressed genes, through comparing KCNG1 high-expression lung cancer tissues with the KCNG1 low-expression lung cancer tissues retrieved from TCGA database. The expression level of KCNG1 mRNA and protein in lung cancer A549 and H1299 cell lines and normal lung epithelial cells were detected by Western blotting and qRT-PCR assays, respectively. The KCNG1 knockdown cell lines were constructed by transfection of siRNA, and the biological behavior changes of the cell lines were detected by EdU, Transwell, Matrigel Transwell, scratch wound healing and angiogenesis mimicry tests, respectively. Results: The expression of KCNG1 mRNA was significantly upregulated in human lung cancer tissues and was correlated with poor prognosis of lung cancer patients (P<0.05). The nomogram model preliminarily confirmed that KCNG1 may be a potential biomarker of lung cancer and has a good prognostic function. GO and KEGG enrichment analysis suggested that KCNG1 plays an important role in regulating hormone secretion, ion transportation, neuropeptide signaling pathway, and intercellular adhesion. GSEA showed that KCNG1 related differentially expressed genes were enriched in KRAS, MTORC1, MYC, P53, and WNT signaling pathways. The proliferation, migration, invasion and tube formation of si-KCNG1 lung cancer cells were significantly inhibited (P<0.05). Conclusion: The expression of KCNG1 mRNA is significantly overexpressed in lung cancer and is closely related to poor prognosis. SiRNA-mediated knockdown of KCNG1 could significantly inhibit the malignant behaviors of lung cancer cell. ［Key words］lung cancer; potassium voltagegated channel modifier subfamily G member 1; molecular therapeutic target; differentially expressed genes; biomarkers
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		2023 Vol. 33 (06): 475-485 [Abstract] ( 16 ) [HTML 1KB] [ PDF 31565KB] ( 504 )

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		2023 Vol. 33 (06): 486-492 [Abstract] ( 17 ) [HTML 1KB] [ PDF 4449KB] ( 508 )

	493	Microflora diversity of upper respiratory tract in children with asthma based on high throughput sequencing

		Objective： To analyze the differences in the types and quantity of bacterial colonization in the upper respiratory tract between the children with asthma and the healthy children. Methods: Eight preschool children with asthma admitted to our hospital from October 2020 to October 2021 were selected, and 18 healthy children who underwent physical examination in our hospital during the same period were selected as the control group. The differences in the species and quantity of respiratory flora between the two groups were analyzed. Results: 16S rDNA sequencing showed that the species abundences and diversity of the upper respiratory tract microbiota in children with asthma increased, and the structure of the microbiota changed significantly. The total number of amplicon sequence variants （ASVs） in the asthma group and the control group was 375, and the unique ASVs were 889 and 1 037, respectively. The asthma group had significant changes in ASVs compared with the control group. At the phylum level, the asthma group had a significant reduction in the relative abundance of Firmicutes (P<0.01) and significant increases in the relative abundance of Actinobacteria, Fusobacteria, and Campilobacterota (P<0.05). At the genus level, the asthma group had a significant reduction in the relative abundance of Streptococcus and Sphingomonas (P<0.05) and a significant increase in the relative abundance of Neisseria (all P<0.05). Conclusion: There are significant differences in the types and quantity of bacterial flora in the upper respiratory tract between children with asthma and healthy children, and the children with asthma have a state of respiratory flora disorder. ［Key words］asthma; children; upper respiratory tract bacteria; bacterial diversity; 16S rDNA sequencing
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		2023 Vol. 33 (06): 493-497 [Abstract] ( 13 ) [HTML 1KB] [ PDF 7298KB] ( 461 )

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		2023 Vol. 33 (06): 498-501，508 [Abstract] ( 16 ) [HTML 1KB] [ PDF 6413KB] ( 545 )

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		2023 Vol. 33 (06): 502-508 [Abstract] ( 14 ) [HTML 1KB] [ PDF 7153KB] ( 480 )

	509	*Effect of aroC* on biological characteristics and pathogenicity of Edwardsiella tarda**

		Objective： To explore the role of aroC in the pathogenic process of Edwardsiella tarda （Et）. Methods: The ΔaroC and CΔaroC of Et were constructed by homologous recombination of suicide plasmid. The effects of aroC on the biological function of Et were analyzed by biofilm formation, motility test, cell adhesion, zebrafish pathogenicity and bacterial colonization. Results: The deletion of aroC gene did not affect the growth rate of Et. Compared with the wild type and the CΔaroC, the biofilm formation, motility, adhesion and extracellular indole production ability of ΔaroC strain decreased significantly (P<0.05 or <0.01). The results of qRT-PCR showed that the transcriptional levels of flagellum synthesis genes fliA and fliC in ΔaroC decreased significantly (P＜0.01), but there was no significant change in the transcriptional levels of flgK, flgL and fliS genes (P＞0.05). Compared with the wild type, symptoms were significantly reduced and higher survival rate were observed in zebrafish infected with ΔaroC, and the colonization number of ΔaroC in intestine and liver of mice was decreased greatly (P<0.05 or <0.01). Conclusion: The aroC can affect the motility, biofilm formation and adhesion, and then regulate the pathogenicity of Et. ［Key words］aroC gene; Edwardsiella tarda; biological characteristics; pathogenicity
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		2023 Vol. 33 (06): 509-515 [Abstract] ( 15 ) [HTML 1KB] [ PDF 15083KB] ( 508 )

	516	*Construction of ChIP-qPCR assay for Vibrio parahaemolyticus* QsvR**

		Objective： To establish the chromatin immunocoprecipitation-real-time quantitative PCR (ChIP-qPCR) assay for Vibrio parahemolyticus QsvR. Methods: The QsvR fragments labeled with 2×Flag tag were transferred into ΔqsvR by heat shock transformation and transformation binding. Arabinose induced the expression of QsvR-2×Flag protein, which was crosslinked with genomic DNA through formaldehyde to form 2×Flag-QsvR-DNA complex. The genomic DNA was ultrasonically cleaved into fragments ranging in size from 100—1 000 bp, and the protein-DNA complex was precipitated by specific antigen-antibody reaction. The cross-linked DNA was de-crosslinked by high salt and heating, and the DNA was recovered for qPCR quantitative DNA to analyze the binding of QsvR and target genes in V. parahemolyticus. Results: The experimental strain ΔqsvR/pBAD33-qsvR-2×Flag was successfully constructed. The IP expression of aphA, opaR, exsB and vtrA of the strain induced by arabinose was significantly higher than that of the control strain. The results indicated that QsvR could bind aphA, opaR, exsB and vtrA in V. parahaemolyticus. Conclusion: The ChIP-qPCR assay of V. parahemolyticus QsvR was established successfully, which can be used to study proteinDNA interaction in prokaryotes. ［Key words］ChIP-qPCR; protein-DNA complex; Vibrio parahaemolyticus; QsvR
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		2023 Vol. 33 (06): 516-520，527 [Abstract] ( 14 ) [HTML 1KB] [ PDF 4579KB] ( 535 )

	521	Predictive value of hepatic steatosis related indexes in type 2 diabetes patients with non-alcoholic fatty liver disease

		Objective： To explore the correlation between hepatic steatosis index (HSI), fatty liver index (FLI) and nonalcoholic fatty liver disease (NAFLD) in type 2 diabetes mellitus(T2DM) population. Methods: A total of 566 subjects with T2DM were included. Clinical data were collected, and they were divided into non-NAFLD group and NAFLD group according to ultrasonography results. General clinical data, HSI and FLI were compared between the two groups. Logistic regression analysis was performed to explore the correlation between HSI，FLI and NAFLD in T2DM patients. The diagnostic value of HSI and FLI were evaluated according to receiver operating characteristic (ROC) curve. Results: The HSI (40.66±5.60 vs 34.58±3.99) and FLI (58.86±21.21 vs 28.96±20.18) in NAFLD group were significantly higher than those in non-NAFLD group (P<0.001). Correlation analysis showed that HSI and FLI were positively correlated with HOMA-IR, waist circumference, triglyceride and total cholesterol, but it was negatively correlated with high density lipoprotein cholesterol (HDL-C). Logistic regression analysis showed that after controlling for metabolic risk factors and hypoglycemic agents, HSI and FLI remained independent risk factors for NAFLD in T2DM patients. The sensitivity and specificity of HSI in diagnosing T2DM with NAFLD were 82.5% and 68.6% respectively. The sensitivity and specificity of FLI in diagnosing T2DM with NAFLD were 86.0% and 70.5% respectively. Conclusion: The HSI and FLI of T2DM patients are calculated according to the conventional biochemical indicators, which is simple and convenient, and may be used to diagnose NAFLD. ［Key words］nonalcoholic fatty liver disease; type 2 diabetes mellitus; hepatic steatosis index; fatty liver index
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		2023 Vol. 33 (06): 521-527 [Abstract] ( 12 ) [HTML 1KB] [ PDF 4881KB] ( 517 )

	528	Application of MRI contrast-enhanced FLAIR sequence in the evaluation of brain metastases

		Objective： To explore the value in detecting brain metastases of contrast-enhanced fluid attenuation inversion recovery sequence （CE-T2 FLAIR） and contrast-enhanced T1WI sequence （CE-T1WI）. Methods： The MR image data of 88 patients with brain metastases admitted to Affiliated People′s Hospital of Jiangsu University from August 2021 to December 2022 with complete clinical data were retrospectively analyzed. The number, length, location, signal intensity, degree of tumor enhancement, and tumor/background ratio of brain metastases in CE-T1WI and CE-T2 FLAIR sequences were compared，and the correlation between the length of the metastases and the rate of enhancement (PI) was analyzed with Pearson correlation analysis. Results： A total of 430 lesions were detected in 88 patients with brain metastases. 340 lesions were shown in CE-T1WI and 420 lesions in CE-T2 FLAIR. There were 80 metastases shown only in the CE-T2 FLAIR sequence, of which 68 were located in the cortical or subcortical regions and 12 were located in the cerebellar hemispheres, most of which were less than 7 mm in diameter. There were 10 metastases shown only in CE-T1WI sequences. Compared with T1WI sequences, the contrast ratio (CR) and contrast signal to noise ratio (CNR) of tumorwhite matter and tumorCSF were apparently higher on T2 FLAIR plain and enhanced sequences (all P＜0.001). The percentage of tumor enhancement in T1WI was apparently higher than that in T2 FLAIR (P＜0.001). In the comparison of white and gray matter metastases at the same scan level, CE-T1WI sequences showed significantly higher PI than CE-T2 FLAIR in both white matter and gray matter (all P＜0.05), and no significant difference in CR between the two sequences. In CE-T1WI sequence, the length of metastatic tumor was significantly correlated with the PI (r=0.831, P＜0.001), but it was not significantly correlated with the PI in CE-T2 FLAIR sequence (r=0.240, P＞0.05). Conclusion： CE-T2 FLAIR sequence shows not only brain metastases shown in CE-T1WI sequence, but also micro-metastases not shown in CE-T1WI sequence, thus, CE-T2 FLAIR should be an essential sequence for MRI detection of brain metastases including micro-metastases. ［Key words］brain metastases； magnetic resonance imaging； contrast-enhanced FLAIR sequence； contrastenhanced T1WI sequence； imaging findings
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		2023 Vol. 33 (06): 528-533 [Abstract] ( 18 ) [HTML 1KB] [ PDF 14431KB] ( 461 )

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		2023 Vol. 33 (06): 534-538，544 [Abstract] ( 14 ) [HTML 1KB] [ PDF 3906KB] ( 550 )

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		2023 Vol. 33 (06): 539-544 [Abstract] ( 12 ) [HTML 1KB] [ PDF 3774KB] ( 482 )

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		2023 Vol. 33 (06): 545-549 [Abstract] ( 9 ) [HTML 1KB] [ PDF 4280KB] ( 505 )

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