Journal of Jiangsu University(Medicine Edition)

[an error occurred while processing this directive]

Journal of Jiangsu University(Medicine Edition)

Home | Instruction for Authors | About Journal | Subscriptions | Advertisement | Contacts Us | 中文

　

Office Online

　

　

　

　

　

　

Editor-in-chief

　

Journal Online

　

　

Forthcoming Articles

　

　

　

　

　

Download Articles

　

　

Quick Search

Advanced Search

Journal of Jiangsu University(Medicine Edition)

	2023 Vol.33 Issue.04 Published 2023-07-19

	1


		2023 Vol. 33 (04): 1- [Abstract] ( 16 ) [HTML 1KB] [ PDF 384KB] ( 378 )

	277	*Neural stem cell secretome inhibited neuroinflammation in vivo* and in vitro by regulating microglial phenotypes**
		ZHOU Jiqin1, NI Wei1, LING Yating1, LYU Xiaorui1, NIU Dongdong1, ZENG Yu1, QIU Yun1, SI Yu1, WANG Ziyu2, HU Jiabo1
		［Abstract］Objective： To explore the role of neural stem cell secretome (NSC-S) on neuroinflammation in vitro and in vivo and its potential mechanisms. Methods： Mouse models of neuroinflammation were developed by lipopolysaccharide (LPS), the effects of NSC-S on the behavior of mice were evaluated by open-field test, and the effects of NSC-S on the microglia phenotype and neuronal injury of cerebral cortex were assessed by immunofluorescence staining and Nissl staining. BV2 microglial inflammatory model was developed by LPS stimulation in vitro. The effect of NSC-S on the phenotype of BV2 cells was detected by flow cytometry. The antiinflammatory mechanism of NSC-S was studied by using peroxisome proliferator activated receptor-γ (PPAR-γ) inhibitor GW9662. Results： The total distance and average movement speed of the mice treated with NSC-S were significantly increased in the open field test (P<0.05), NSC-S regulated the phenotypic polarization of microglia and inhibited neuronal injury in mouse cortex. In vitro, NSC-S inhibited the expression of M1 surface marker CD86 (P<0.05) and promoted the expression of M2 surface marker CD206 (P<0.01) in BV2 cells, however, GW9662 blocked the regulation effect of NSC-S on BV2 cells phenotypes (P<0.05). Conclusion： NSC-S plays a protective role in LPS-induced neuroinflammation in vivo and in vitro by regulating microglia phenotypes.
		2023 Vol. 33 (04): 277-283 [Abstract] ( 31 ) [HTML 1KB] [ PDF 3839KB] ( 670 )

	284	Protective effect of neural stem cell-conditioned medium on neuronal injury in Parkinson′s disease model
		NI Wei1, ZHOU Jiqin1, LING Yating1, LYU Xiaorui1, NIU Dongdong1, ZENG Yu1, QIU Yun1, SI Yu1, WANG Ziyu2, HU Jiabo1
		［Abstract］Objective： To investigate the protective effect of neural stem cell-conditioned medium (NSC-CM) on 6-hydroxydopamine (6-OHDA)-induced neuronal injury in vitro and in vivo model of Parkinson′s disease(PD) and its potential mechanisms. Methods： The in vivo PD model was established by stereotaxic injection of 6-OHDA into the medial forebrain bundle of SD rats, and NSC-CM was injected into the substantia nigra and striatum for treatment. The loss of dopaminergic neuron (DAN) in the substantia nigra and striatum was detected by immunohistochemical staining. The expression level of mitofusin 1 (Mfn1) in substantia nigra was detected by Western blotting; The PD cell model was established in vitro by treating rat adrenal medulla pheochromocytoma (PC12 cells) with 6-OHDA, and pretreated with NSC-CM. Lactate dehydrogenase (LDH) release and cellular reactive oxygen species (ROS) levels in PD cell model were detected, and the expression level of Mfn1 was detected by Western blotting. The components of NSC-CM were detected by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and analyzed by bioinformatics analysis. Flow cytometry and laser confocal microscopy were used to observe the internalization of neural stem cells (NSCs) mitochondria by PC12 cells. The effects of NSC-CM on the cell viability and mitochondrial fluorescence intensity of PC12 cells were compared before and after removal of mitochondria. Results： After injection of NSC-CM, the loss of DAN was significantly decreased and the expression level of Mfn1 protein was greatly increased in PD rats (P<0.05). After NSC-CM pretreatment, the release of LDH and the level of ROS in the PD cell model were significantly decreased (P<0.05), and the expression level of Mfn1 protein was remarkably increased (P<0.05). LC-MS/MS detected the presence of mitochondrial components in NSC-CM, and observed the internalization of mitochondria in NSC-CM by PC12 cells. Removal of mitochondria from NSC-CM significantly decreased the survival rate of PC12 cells (P<0.05) and the fluorescence intensity of mitochondria (P<0.05). Conclusion： NSC-CM could improve neuronal death, increased oxidative stress level and decreased mitochondrial fusion level caused by 6-OHDA, and this protective effect may be related to PC12 cells internalizing NSCs mitochondria.
		2023 Vol. 33 (04): 284-291 [Abstract] ( 13 ) [HTML 1KB] [ PDF 3751KB] ( 447 )

	292	Butylphthalide improves the cognitive function of APP/PS1/Tau mice by up-regulating the expression of IGF1R in hippocampus
		XU Man, YU Ming
		Objective： To observe the effect of butylphthalide (NBP) on the expression of insulin-like growth factor 1 receptor (IGF1R) in hippocampus of APP/PS1/Tau mice, and to explore the mechanism of IGF1R in NBP treatment of Alzheimer′s disease (AD). Methods： Thirty 8-month-old male APP/PS1/Tau mice were randomly divided into AD group, NBP intervention group and NBP+IGF1R inhibitor intervention group, with 10 mice in each group. Ten 8-month-old C57BL/6J male rats were also taken as the control group. The control group and AD group were given 0.2 mL of vegetable oil by gavage daily, the NBP intervention group was given 120 mg/kg of NBP by gavage daily, and the NBP+IGF1R inhibitor intervention group was given 10 mg/kg of IGF1R inhibitor (NVP-ADW742) by intraperitoneal injection on the basis of the NBP intervention group. After 6 weeks of continuous administration, cognitive behavior of each group was detected by Y maze test, new object recognition test and water maze test. The expressions of Aβ, Tau and p-Tau in hippocampus were detected by Western blotting, Nissl staining was used to evaluate the damage of hippocampal neurons, and immunofluorescence was used to detect the expression of IGF1R in hippocampus. Results： Compared with AD group, NBP intervention group had higher spontaneous alternation rate, higher cognitive index (P<0.05), shorter escape latency of water maze and more times of crossing the platform (P<0.05). Compared with AD group, the protein Aβ and p-Tau in hippocampus of NBP intervention group decreased significantly (P<0.05), the apoptosis of hippocampal neurons decreased significantly (P<0.05) and the expression of IGF1R in hippocampus increased significantly (P<0.05). Compared with NBP intervention group, the expressions of Aβ and p-Tau protein in hippocampus of NBP+IGF1R inhibitor intervention group were higher (P<0.05), the spontaneous alternation rate and cognitive index were significantly decreased (P<0.05), the latency of water maze escape was remarkably prolonged (P<0.05), and the number of crossing platforms was remarkably reduced (P<0.05). Conclusion： NBP could protect AD by up-regulating the expression of IGF1R in hippocampus.
		2023 Vol. 33 (04): 292-296，302 [Abstract] ( 20 ) [HTML 1KB] [ PDF 2635KB] ( 408 )

	297


		2023 Vol. 33 (04): 297-302 [Abstract] ( 14 ) [HTML 1KB] [ PDF 785KB] ( 400 )

	303	p53 lactylation promotes the proliferation and metastasis of colon cancer cells
		DAI Yao1, DAI Haiyan1, DA Wenxin1, WANG Yahui1, ZHANG Ziqi2, WANG Shengjun1, MA Jie1
		Objective： To investigate the effect and mechanism of p53 lactylation on the proliferation and metastasis of colon cancer cells. Methods： Human colon cancer tissues and corresponding adjacent normal tissues were collected, and the pathological morphology was confirmed by hematoxylin-eosin staining. Lactate concentration was detected by lactate detection kit, and the expression of lactylation and p53 were detected by immunohistochemical staining. The subcellular localization of lactylation and p53 in HCT116 cells were observed by immunofluorescence, and p53 lactylation was detected by immunocoprecipitation. Subsequently, endogenous lactate was used to mediate p53 lactylation of HCT116 cells and the lactate inhibitor oxamate was used to inhibit p53 lactylation. The proliferation ability of HCT116 cells was detected by CCK-8. The migration and invasion ability of HCT116 cells were detected by scratch and Transwell assay. Results： The expression of lactylation and p53 in human colon cancer tissue was higher than that in adjacent normal colon cancer tissue, and p53 lactylation was clearly observed. Endogenous lactate in HCT116 cells can mediate p53 lactylation, inhibit p53 entry into the nucleus, and promote cell proliferation，migration and invasion. Conclusion： p53 lactylation promotes proliferation and metastasis of colon cancer cells by inhibiting the nuclear translocation of p53.
		2023 Vol. 33 (04): 303-309 [Abstract] ( 23 ) [HTML 1KB] [ PDF 3705KB] ( 747 )

	310	Bioinformatics analysis of human ferredoxin 1, a key regulatory gene for cuproptosis
		GUAN Yunliang1,2
		Objective： To analyze the gene structure and characteristics of ferredoxin 1 (FDX1), a key regulatory gene for cuproptosis, using bioinformatics methods. Methods： The human FDX1 DNA sequence was obtained from GenBank database, and bioinformatics analysis of FDX1 general characteristics, structural recognition, characteristic analysis, and tissue expression was conducted by using data mining software. Results： The human FDX1 gene is located at 11q22.3, with a total length of 35 553 bp, strong codon bias, and the repetitive sequence accounting for 61.40% of the total length of the gene. There are 5 promoters (score>0.90), 71 transcription factor binding sites and polyadenylation tail signal at site 518 in the upstream 2 000 bp region, and no CpG island can be predicted. Subcellular localization of FDX1 is most likely in the mitochondria. The molecular function prediction showed that FDX1 had 2Fe-2S cluster binding activity. The maximum mRNA of human FDX1 gene expression was in the adrenal gland ［146(100×FPKM)1/2］. The cancer specificity of FDX1 mRNA in each tissue is low. Conclusion： The mitochondrial subcellular localization, 2Fe-2S cluster activity, and involved signaling pathways of FDX1 have been confirmed that FDX1 could be used as a key regulatory genes for cuproptosis from the perspective of biological algorithms.
		2023 Vol. 33 (04): 310-315，322 [Abstract] ( 17 ) [HTML 1KB] [ PDF 5735KB] ( 678 )

	317	comThe role of circ_0000515/miR-1296-5p/CDK2 axis in breast cancer
		YANG Qin1#, WANG Chao2#, LIN Bohan2, YU Qiwen2, LI Tao2, SUN Xiaochun2
		Objective： To explore the role and underlying mechanism of circ_0000515 in the progression of breast cancer. Methods： The circRNAs chip dataset (GSE101123) of breast cancer was downloaded from GEO database and analyzed by R language. The target circRNA was selected and its expression in breast cancer cell lines and tissues were detected. The target miRNA of the circRNA and the target mRNA of the miRNA were selected by bioinformatics analysis, and verified by double luciferase experiment and Western blotting. The siRNA of circRNA was transfected into MCF-7 and MDA-MB-231. The cell proliferation and migration were measured by CCK-8 and scratch assay. The expression of RNA were detected by qRT-PCR. The expression of mRNAs-encoded protein was detected by Western blotting. Results： GSE101123 dataset was selected and downloaded. Five highly expressed circRNAs molecules were screened by R language, and circ_0000515 was selected as the research object for subsequent study. Bioinformatics analysis showed that there may be a targeted relationship among circ_0000515, miR12965p and cell cycledependent kinase 2 (CDK2). qRT-PCR results showed that the expression of circ_0000515 in breast cancer cell lines was higher than that in normal cell lines, and its expression in cancer tissues was higher than that in paracancerous tissues. After knockdown the expression of circ_0000515, the proliferation and migration ability of breast cancer cells decreased significantly, while the expression of miR-1296-5p increased. Double luciferase assay confirmed that there was a targeted relationship between circ_0000515 and miR-1296-5p. Western blotting showed that the expression of CDK2 decreased after knocking down the expression of circ_0000515. Conclusion： circ_0000515 is highly expressed in breast cancer. circ_0000515/miR-1296-5p/CDK2 axis may play a role in the progression of breast cancer.
		2023 Vol. 33 (04): 317-322 [Abstract] ( 23 ) [HTML 1KB] [ PDF 1202KB] ( 532 )

	323	The value of artificial intelligence in post-processing coronary CTA images and diagnosing coronary artery stenosis
		QI Dong, YAO Chuanshun, HU Shumin, HE Xingyi, YAO Muzi, SHEN Chaojun
		Objective： To analyze the value of artificial intelligence (AI) for coronary CT angiography (CCTA) image postprocessing and diagnosis of coronary artery disease. Methods： The imaging data of 40 patients who both underwent CCTA and percutaneous coronary arteriography (CAG) from January 2020 to January 2022 were retrospectively collected and analyzed. Image postprocessing and diagnosis were divided into manual group and AI group to compare the differences in postprocessing time and subjective image quality scores and to assess the differences in coronary plaque properties. Using CAG results as the gold standard, the differences in sensitivity, specificity, positive predictive value, negative predictive value and accuracy in the diagnosis of coronary artery stenosis between the two were compared on a vessel-by-vessel basis, and the consistency of the results was evaluated using the Kappa test. Results： The postprocessing and diagnosis time in the AI group was (236.57±20.66)s, which was approximately 70.04% shorter than that in the manual group (789.74±63.38)s. The difference was statistically significant (P<0.05); The difference between the objective and subjective scores of image quality obtained by the two methods was not statistically significant (all P>0.05). The overall accuracy of plaque detection in the AI group was 96.32% (131/136). The differences in the detection of calcified coronary plaque, noncalcified plaque and mixed plaque between the manual and AI groups were not statistically significant (P>0.05) and were in good agreement (Kappa=0.901, P<0.001). On a vesselbyvessel basis, the sensitivity, specificity, positive predictive value, negative predictive value and accuracy of the AI group in diagnosing coronary artery stenosis were 87.72% (50/57), 94.12% (48/51), 94.34% (50/53), 87.27% (50/53) and 90.74% (98/108), respectively, and the CAG in diagnosing coronary artery stenosis with good consistency (Kappa=0.815, P<0.001). Conclusion： AI has certain advantages in CCTA image post-processing efficiency, plaque nature identification and coronary stenosis, and can be used as an effective aid for physicians to analyze and diagnose coronary artery disease.
		2023 Vol. 33 (04): 323-327,332 [Abstract] ( 19 ) [HTML 1KB] [ PDF 967KB] ( 432 )

	328	Diagnostic value of small field thin-section target reconstruction of CT in adrenal disorders
		ZHOU Chenyi1, WANG Liangliang1, LIU Hui1, XU Daowen1, YE Na2, WANG Yanling1, SUN Gang1
		Objective： To compare the clinical value of conventional vision and small field thinsection target reconstruction CT technique in adrenal disorders. Methods： Between April 2018 and December 2021, 97 patients underwent both conventional scan and target reconstruction CT examinations at the People′s Hospital of Suzhou New District were included. Differences in image quality and diagnostic efficacy between conventional method and small field methods were analyzed according to signal noise ratio (SNR), contrast noise ratio (CNR) and image evaluation of image quality by two senior radiologists. Image quality indicators and subjective scores were statistically analyzed using the paired t-test and Wilcoxon tests. Image features and diagnostic coincidence rates were statistically analyzed using the Chi-square test. Results： SNR, CNR and image quality scores of small field method were significantly higher than those in conventional method (P<0.001). The coincidence rates of positioning and qualitative diagnosis of small field method were increased by 25.58% and 23.25% respectively compared with conventional method (P<0.05). There were statistically significant differences in adrenal status between the small field method and conventional method (P<0.05). Conclusion： Small field target reconstruction of CT images are conducive to the display of morphologic change and may be used to improve the diagnostic accuracy of adrenal disorders.
		2023 Vol. 33 (04): 328-332 [Abstract] ( 19 ) [HTML 1KB] [ PDF 1250KB] ( 381 )

	333	Evaluation of the effect of Leucogen on neutropenia in children with influenza virus infection
		LIU Huan, WANG Suoying, LI Hong
		Objective： To evaluate the efficacy of Leucogen in the treatment of influenza virus infection with neutropenia in children. Methods： Sixtythree cases of influenza virus infection combined with neutropenia in the Department of Pediatrics, Affiliated Hospital of Jiangsu University from January 2021 to January 2022 were collected. They were randomly divided into the observation group (33 cases) and the control group (30 cases). Eight serum respiratory pathogens, seven throat swab respiratory pathogens, blood routine examination, C-reactive protein, procalcitonin and blood culture were detected in the two groups. On the basis of routine treatment such as antiviral and symptomatic treatment, the observation group was given different doses of Leucogen orally according to ages. The changes of white blood cells and neutrophils were observed at different time points. Results： On the 5th day after treatment, white blood cells and neutrophils of the two groups were significantly higher than those before treatment (both P<0.01). When compared with the control group, the increase of white blood cells and neutrophils in the observation group was more significant after treatment with Leucogen (both P<0.05). When compared with the control group, the total effective rates of white blood cells and neutrophils elevation in the observation group were significantly different after treatment with Leucogen at 3 days, 5 days and 7 days (P<0.01). When compared with the control group, the obvious effect rates of white blood cells and neutrophils elevation were not significant at 3 days after Leucogen treatment, but the obvious effect rates of white blood cells and neutrophils elevation were statistically significant at 5 and 7 days (both P<0.05). Conclusion： After the treatment of influenza virus infection with neutropenia in children, Leucogen could obviously increase leukocytes and neutrophils, and may consequently shorten the window period of secondary bacterial infection.
		2023 Vol. 33 (04): 333-337 [Abstract] ( 26 ) [HTML 1KB] [ PDF 738KB] ( 445 )

	338	Efficacy of growth hormone in children with short stature complicated with benign thyroid nodules
		ZHU Yanli1,2, TANG Aimei1, GU Qi1, TAN Juan1, LI Yue′e1, XU Yuanyuan3, WANG Jie3, TANG Yongquan3, ZHOU Wendi1,3
		Objective： To observe the effect of growth hormone on children with short stature complicated with benign thyroid nodules. Methods： Forty-seven children with short stature complicated with benign thyroid nodules who received growth hormone treatment in the Department of Pediatrics, Huai′an Clinical College of Xuzhou Medical University from January 2021 to October 2022 were selected as the treatment group. Thirty short stature children whose parents refused to receive growth hormone treatment were randomly selected as the control group. The changes of height and thyroid function were observed at the first visit, the 6th month and the 12th month. Results： The height and growth rate were significantly increased and the height standard deviation scores (HtSDS) were improved after 6 and 12 months treatment with growth hormone, the levels of insulinlike growth factor-1 (IGF-1) were significantly higher than those in the controls (P<0.05). In the control group, the height at the 6th and 12th month of follow-up was higher than that at the first visit (P<0.05), but the increase was not as significant as that in the treatment group. Growth rate, HtSDS and IGF-1 levels had no significant changes before and after re-diagnosis. In the treatment group, the maximum cross-sectional diameter and grade of benign thyroid nodule had no significant change after treatment (P>0.05), and the level of FT4 had no significant difference before and after treatment, the levels of TSH and FT3 were in the normal range after treatment. Conclusion： Growth hormone therapy could effectively improve the height of short children with benign thyroid nodules, and produce no significant effect on benign thyroid nodules. It is recommended to use growth hormone therapy for such children.
		2023 Vol. 33 (04): 338-342 [Abstract] ( 25 ) [HTML 1KB] [ PDF 748KB] ( 383 )

	343


		2023 Vol. 33 (04): 343-347 [Abstract] ( 13 ) [HTML 1KB] [ PDF 1604KB] ( 431 )

	348


		2023 Vol. 33 (04): 348-351 [Abstract] ( 16 ) [HTML 1KB] [ PDF 1669KB] ( 477 )

	352


		2023 Vol. 33 (04): 352-358 [Abstract] ( 15 ) [HTML 1KB] [ PDF 942KB] ( 427 )

	359


		2023 Vol. 33 (04): 359-363，368 [Abstract] ( 15 ) [HTML 1KB] [ PDF 1311KB] ( 543 )

	364


		2023 Vol. 33 (04): 364-368 [Abstract] ( 21 ) [HTML 1KB] [ PDF 771KB] ( 410 )

江苏大学学报:医学版

　

News

　

　　　　　　　　　　More

　

Links

　

　　　　　　　　　　More

Copyright © 2011 Journal of Jiangsu University(Medicine Edition)
Editorial Department of Journal of Jiangsu University E-mail：xbyx@ujs.edu.cn