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Journal of Jiangsu University(Medicine Edition)
 
2022 Vol.32 Issue.06
Published 2022-10-18
0
2022 Vol. 32 (06): 0- [Abstract] ( 82 ) [HTML 1KB] [ PDF 387KB] ( 1321 )
461 Clinicopathological characteristics of patients with papillary thyroid carcinoma  combined with breast cancer
ZHAO Yuanyuan, HE Qi, JI Feihong, et al.
Objective： To analyse the clinicopathological features of patients with papillary thyroid carcinoma (PTC) combined with breast cancer and to explore the possible relationship between these two cancers. Methods： A total of 99 patients with PTC combined with breast cancer (combined cancer group) who were admitted to the Thyroid Surgery Department of the First Affiliated Hospital of Zhengzhou University from December 2016 to December 2021 were selected, and 99 patients with PTC without breast nodule (simple cancer group) who were treated during the same period were selected. The general characteristics, PTC pathological characteristics, thyroid hormone, antigen and antibody levels were compared between the two groups. According to the order of diagnosis and the interval time, the patients with PTC combined with breast cancer were divided into breast-thyroid group (diagnosed with PTC after breast cancer), thyroid-breast group (diagnosed with breast cancer after PTC) and simultaneous group (the interval between the diagnosis of two cancers did not exceed 6 months). The age at diagnosis of PTC and breast cancer, the interval between diagnosis of two cancers, the lymph node metastasis of PTC and pathological characteristics of breast cancer were compared among the three groups. Results： Compared with the simple cancer group, the tumor in the combined cancer group was smaller in maximum diameter, more inclined to microcarcinoma, and less likely to invade the capsule and surrounding tissues; the age at the time of diagnosis of PTC was significantly older, most of them were in menopausal state; the level of serum free tetraiodothyronine (FT4) was also significantly decreased, while the level of thyroglobulin (Tg), thyroid peroxidase antibody (TPOAb) and thyrotropin receptor antibody (TRAb) were significantly increased (all P<0.05). Compared with the thyroid-breast group, the interval between diagnosis of two cancers in the breast-thyroid group was significantly shorter (P<0.05). Compared with the simultaneous group, the rate of cervical lymph node metastasis in the breast-thyroid group was significantly higher (P<0.05). Conclusion： Thyroid tumors in patients with PTC combined with breast cancer were smaller in maximum diameter, and some patients developed rapidly and were prone to skip metastasis; PTC patients with high expression level of Tg, TPOAb and TRAb had an increased risk of breast cancer.
［Key words］papillary thyroid carcinoma; breast cancer; thyroid hormone; thyroglobulin; thyroid associated antibody; lymph node metastasis; microcarcinoma
2022 Vol. 32 (06): 461-466 [Abstract] ( 20 ) [HTML 1KB] [ PDF 4086KB] ( 476 )
467
2022 Vol. 32 (06): 467-470 [Abstract] ( 26 ) [HTML 1KB] [ PDF 2637KB] ( 576 )
471
2022 Vol. 32 (06): 471-476 [Abstract] ( 30 ) [HTML 1KB] [ PDF 3955KB] ( 547 )
477
2022 Vol. 32 (06): 477-482 [Abstract] ( 34 ) [HTML 1KB] [ PDF 3926KB] ( 821 )
483
2022 Vol. 32 (06): 483-488，496 [Abstract] ( 32 ) [HTML 1KB] [ PDF 4677KB] ( 426 )
489 PGC-1α as a potential therapeutic target for Alzheimer′s disease with  a particular focus on OPA1 expression
Objective： To explore the role of peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α) in improving Alzheimer′s disease (AD), as well as its potential mechanisms with a particulars focus on optic atrophy 1 (OPA1) regulation. Methods： In vitro, AD models were constructed by transfecting APPswe plasmids into N2A cells. qRT-PCR was used to evaluate the impact of mutAPP overexpression on the varies of PGC-1α and OPA1 transcription. APPswe and PGC-1α plasmids were cotransfected into N2A cells, regulation effect of PGC-1α on the levels of OPA1 transcription were evaluated by qRT-PCR during AD. In vivo, by infusing AAV-PGC1α into the lateral parietal association (LPtA) cortex of APP/PS1 brain, we successfully overexpressed  PGC-1α in the LPtA of APP/PS1 mouse. The regulatory effect of PGC1α on the expression of OPA1 and on the morphological changes of mitochondrial inner membrane during AD were examined by immunohistochemistry and transmission electron microscopy respectively. Results： Paralleling AD-like Aβ deposits, a significant reduction in the levels of PGC-1α and OPA1 was observed in APP/PS1 mice and APPswe transfected cells (all P<0.01). Furthermore, overexpression PGC-1a in LPtA rescued the mitochondrial damage, especially on damaged mitochondrial inner membrane in AD. Notably, upregulated expression of OPA1 were available in the LPtA cortex of APP/PS1 mice (all P<0.01) . Conclusion： PGC-1α contributes to the regulation of the mitochondrial fusion, thereby affecting Aβ aggregate in AD. Elevating of the PGC-1α level, either pharmacologically or through other means, could present a new therapeutic strategy for AD.
［Key words］Alzheimer′s disease; APP/PS1 mice; peroxisome proliferator-activated receptor γ coactivator-1α(PGC-1α); optic atrophy 1(OPA1); mitochondria; fusion protein
2022 Vol. 32 (06): 489-496 [Abstract] ( 31 ) [HTML 1KB] [ PDF 35074KB] ( 505 )
497 L-Wnt3a cell culture system with increased contents of Wnt3a in supernatant and its efficacy of inducing differentiation of pluripotent stem cells into melanocytes
Objective： To optimize the collection protocol and calibrate the Wnt3a protein concentration of L-Wnt3a conditioned medium (L-Wnt3a CM) and verify its role as a source of Wnt3a in pluripotent stem cell (PSC) to melanocytes differentiation system. Methods： The growth characteristics of L-Wnt3a cells and the characteristics of their secreted Wnt3a protein amount were observed to set the parameters of the collection method of L-Wnt3a conditioned medium, and the collected conditioned medium was calibrated for Wnt3a protein concentration using ELISA, and melanocyte differentiation medium with a defined Wnt3a concentration was formulated for the embryonic stem cell line WA09 and the induced pluripotent stem cell line WTC-11 cells were subjected to suspension-induced differentiation of melanocytes, and the obtained induced melanocytes were subjected to melanin gene expression assay and Masson-Fontana staining. Results： The new collection system was able to obtain Wnt3a protein stably and efficiently, and the differentiation system with a defined Wnt3a concentration was able to induce melanocytes successfully in WA09 and WTC-11, and the corresponding concentration of recombinant Wnt3a protein was able to achieve similar results. Conclusion： The precise concentration of L-Wnt3a CM could be efficiently obtained by optimizing the collection protocol and the use of a culture system with defined Wnt3a concentration also successfully induced of PSC differentiation into functional melanocytes.
［Key words］melanocytes; pluripotent stem cells; Wnt3a protein; induced pluripotent stem cells; cell differentiation;
2022 Vol. 32 (06): 497-503 [Abstract] ( 29 ) [HTML 1KB] [ PDF 10780KB] ( 462 )
504 Changes and clinical significance of myeloid-derived suppressor cells in peripheral blood of patients with chronic sinusitis with nasal polyps
WU Huihui,YANG Jun,ZENG Xuanfu,et al.
Objective： To investigate the changes in the ratio and function of myeloidderived suppressor cells (MDSCs) and its subgroups in patients with chronic sinusitis with nasal polyp (CRSwNP), and explore the influence of MDSCs on the occurrence and development of CRSwNP. Methods： Flow cytometry (FCM) was used to detect the ratio of HLA-DR-CD11b+CD33+ MDSCs, HLA-DR-CD11b+CD33+CD14-CD15+ PMN-MDSCs and HLA-DR-CD11b+CD33+CD14+CD15- M-MDSC, and the expression of the functional molecule arginase-1 (ARG-1) in peripheral blood in the CRSwNP group (31 cases) and control group (36 cases). At the same time, the concentration of IL-6 in plasma was detected by enzyme-linked immunosorbent assay. Results： The proportion of total MDSCs in the peripheral blood of CRSwNP patients was significantly higher than that of the control group (P<0.01). Among them, the proportion of PMN-MDSCs in the CRSwNP group was significantly increased (P<0.05); while the proportion of M-MDSCs decreased (P<0.05). The average fluorescence intensity of ARG-1 in the total MDSCs in the CRSwNP group increased (P<0.05), and the plasma IL-6 concentration also increased (P<0.05). Conclusion： The expression of total MDSCs in CRSwNP patients is increased and PMN-MDSCs are the dominant ones, and the expression of ARG-1 is also increased. It suggests that MDSCs may be involved in the occurrence and development of chronic sinusitis.
［Key words］chronic sinusitis; nasal polyps; myeloid-derived suppressor cells and their subgroups; immunosuppression; arginase-1; interleukin-6
2022 Vol. 32 (06): 504-508 [Abstract] ( 25 ) [HTML 1KB] [ PDF 3848KB] ( 547 )
509 Changes and influencing factors of angle Kappa in cataract patients before and after MIOL implantation surgery
Objective： To compare the changes and influencing factors of angle Kappa before and after cataract surgery. To explore the setting standard of Kappa angle when choosing multifocal intraocular lens (MIOL) in cataract patients. Methods： This was a retrospective study. The angle Kappa was measured before and after surgery in patients who underwent cataract phacoemulsification combined with posterior chamber MIOL implantation in the Ophthalmology Department of West China Hospital of Sichuan University from September 2017 to January 2020. The distribution characteristics and changes of angle Kappa in cataract patients were analyzed, and the correlation between angle Kappa and ocular biometric parameters was analyzed by Pearson correlation, and multiple linear regression analysis was used to explore the influencing factors of angle Kappa. Results： ① Locations of angle Kappa of before and after surgery in cataract patients were scattered mostly in the temporal side of corneal light reflection, and the direction shifted to the nasal side after surgery. ② The value of angle Kappa before cataract surgery was (0.237±0.134) mm. The preoperative angle Kappa was positively correlated with pupil diameter (r=0.186, P<0.05), and negatively correlated with anterior chamber depth, keratometry K1 and K2 (r=-0.268，-0.279，-0.254，all P<0.05). ③ The value of angle Kappa after cataract surgery was (0.208±0.112) mm. The postoperative angle Kappa was positively correlated with the pupil diameter (r=0.253, P<0.05). ④ The effects of anterior chamber depth（b=-0.092，t=-3.679，P＜0.001） and pupil diameter（b=0.036，t=2.735，P=0.007） on the value of angle Kappa before the surgery were statistically significant; The influence of preoperative angle Kappa on postoperative angle Kappa was statistically significant (b=0.491, t=8.608, P<0.001). Conclusion：Cataract surgery may decrease the value of angle Kappa. Preoperatively, the value of angle Kappa was negatively correlated with anterior chamber depth. Therefore, MIOL implantation should be carefully chosen for patients with shallow anterior chamber.
［Key words］angle Kappa; cataract; multifocal intraocular lens; iTrace visual function analyzer; anterior chamber depth； corneal curvature
2022 Vol. 32 (06): 509-514 [Abstract] ( 29 ) [HTML 1KB] [ PDF 5796KB] ( 805 )
515 Effect of resting metabolic rate on peripheral neuropathy in patients with type 2 diabetes
SHI Li, LIU Xuekui, XU Wei,et al.
Objective： To study the correlation between resting metabolic rate (RMR) and diabetic peripheral neuropathy (DPN) in adult patients with type 2 diabetes mellitus（T2DM）, and to evaluate the modifying effects of BMI, waist circumference, neck circumference, waist-to-hip ratio and body fat on the correlationship. Methods： A total of 416 patients with type 2 diabetes were selected and divided into two groups: single T2DM group and T2DM+DPN group. The subjects′ height, body weight, waist circumference, neck circumference, hip circumference, and nerve conduction velocity were measured, and the blood lipid, blood pressure, fasting insulin, blood glucose, HbAlc, and other biochemical indicators were examined. Mifflin-St-Jeor formula was used to calculate RMR, and RMR was stratified by the quartile method. The Logistic regression model was used to analyze the relationship between RMR and DPN. Waist circumference, hip circumference, waist-to-hip ratio, and bady fat data were stratified into three grades, and multiple stepwise Logistic regression analyses were applied to analyze the effects of RMR on DPN at different levels. Results： The risk of DPN decreased with the increase of RMR level in T2DM (P<0.05). Stratified analysis showed that RMR had significant effect on DPN when waist circumference<90 cm or>98 cm, neck circumference>101 cm, waist-to-hip ratio<0.92 and body fat<24.8%（all P<0.05）. When T2DM was complicated with obesity, the risk of DPN was decreased following with the increasing RMR (P<0.05) and there was an interaction (P=0.021). Conclusion：RMR of T2DM patients is associated with DPN, and the higher the RMR, the lower the risk of DPN.
［Key words］type 2 diabetes mellitus; resting metabolic rate; diabetic peripheral neuropathy；obesity；interaction
2022 Vol. 32 (06): 515-519，523 [Abstract] ( 27 ) [HTML 1KB] [ PDF 4220KB] ( 479 )
520
2022 Vol. 32 (06): 520-523 [Abstract] ( 30 ) [HTML 1KB] [ PDF 3824KB] ( 508 )
524 Establishment of a method determining the contents of seven anti-inflammatory active components of the Miao medicine Inula cappa in Raw 264.7 cells
ZHOU Jie, XUE Cun, ZHANG Qing, et al.
Objective： To establish a method simultaneously determining the contents of seven anti-inflammatory active components including luteolin (LUT), chlorogenic acid (CA), and neochlorogenic acid (NCA), cryptochlorogenic acid (CCA), 3,4-dicaffeoylquinic acid (3,4-DCQA), 3,5-dicaffeoylquinic acid (3,5-DCQA), 4,5-dicaffeoylquinic acid (4,5-DCQA) of the Miao medicine Inula cappa in Raw264.7 cells. Methods： Using mouse macrophage Raw264.7 as the research object, ultra-high liquid chromatography-triple quadrupole mass spectrometry (UPLC-MS/MS) was used to establish the simultaneous determination of LUT, CA, NCA, CCA, 3,4-DCQA, 3,5-DCQA, 4,5-DCQA methods for the content of seven components of the Miao medicine Inula cappa, including specificity, linearity, matrix effect, recovery, accuracy, precision and stability. Results： A rapid and stable analytical method for the measurement of trace drugs in Raw264.7 cells was established. The lowest limit of quantification of the method was 0.48-3.84 ng/mL, and the accuracy was (90.78±5.30)%-(113.99±13.51)%. Its extraction recovery was (87.19±5.33)%-(103.59±14.00)% and the matrix effect was (94.50±15.17)% - (112.77±7.02)%. The stability was (91.71±11.70)%-(109.45±10.63)%, which could meet the measurement requirements. Conclusion： A rapid and accurate method for simultaneous determination content of seven active components LUT, CA, NCA, CCA, 3,4-DCQA, 3,5-DCQA, 4,5-CQA using UPLC-MS/MS was established, which could provide a reference for the study of cellular pharmacokinetics of Inula cappa extract.
［Key words］Inula cappa extract; Raw264.7 cells; anti-inflammatory components； chlorogenic acid; cell pharmacokinetics; UPLC-MS/MS
2022 Vol. 32 (06): 524-531 [Abstract] ( 23 ) [HTML 1KB] [ PDF 6781KB] ( 511 )
532
2022 Vol. 32 (06): 532-536 [Abstract] ( 34 ) [HTML 1KB] [ PDF 3524KB] ( 512 )
537
2022 Vol. 32 (06): 537-541，545 [Abstract] ( 32 ) [HTML 1KB] [ PDF 4201KB] ( 660 )
542
2022 Vol. 32 (06): 542-545 [Abstract] ( 35 ) [HTML 1KB] [ PDF 2519KB] ( 589 )
546
2022 Vol. 32 (06): 546-549 [Abstract] ( 41 ) [HTML 1KB] [ PDF 2656KB] ( 493 )
江苏大学学报:医学版
　

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