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Journal of Jiangsu University(Medicine Edition)

	2022 Vol.22 Issue.1 Published 2022-01-30

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		2022 Vol. 22 (1): 0- [Abstract] ( 52 ) [HTML 1KB] [ PDF 402KB] ( 545 )

	1	Screening of potential therapeutic compounds for osteoporosis based on L1000 database
	Screening of potential therapeutic compounds for osteoporosis based on L1000 database[J]. Journal of Jiangsu University(Medicine Edition), 2022,22(1): 1-7,12>')" href="#">	LIANG Pengchen1,ZHOU Ziyan2,3, CHANG Qing2, YI Qingqing2, SUN Miaomiao2,3, TANG Yeling2,3, CAO Liou4,5, YANG Jie6
		Objective:The L1000 micro-interference data set was used to screen compounds for the treatment of osteoporosis, and the evaluation and cytological activity verification by multiple biological information methods were conducted, aiming to discover new osteoporosis treatment drugs.Methods:The data related to osteoporosis（OP）were retrieved in the GEO（Gene Expression Omnibus）database, and the difference in gene expression levels on the datasets was analyzed using limma package in R language. Pathway enrichment analysis of differential genes was performed using MetaScape data platform. The resulting differential OP gene expression profiles were analyzed to match potential OP therapeutic compounds in the L1000 dataset of the Connectivity Map database. The molecular docking study of the matching compounds and bone metabolism targets was conducted by Autodock_Vina software, and the ADMET（absorption, distribution, metabolism, excretion, and toxicity）characteristics of the matching compounds were calculated and analyzed by using pkCSM database. The activity of the candidate compound was studied by a cell proliferative activity experiment.Results:After analyzing the dataset of osteoporosis expression profile, 195 differential genes related to osteoporosis were obtained, including 127 genes with significantly up-regulated expression and 68 genes with significantly downregulated expression. There were 146 GO Biological Processes with differential gene enrichment;There were nine enriched KEGG signaling pathways and nine enriched Reactome Gene Sets. A total of 10 potential therapeutic compounds were matched in the L1000 dataset: diethylstilbestrol, PLX-4720, mezlocillin, KU-C103428N, rhapontin, maravir, quinine, MST-312, risperidone, and CO-102862. Through molecular docking studies, we found that the matching compounds have multi-target characteristics, and the main docking activities with the compounds are CAⅡ, PGR, ERβ, BMP2 and other OP targets. The ADMET properties of potential therapeutic compounds were calculated using the ADMET calculation module of the pkCSM database, which showed that mezlocillin had good ADME performance and low toxicity, with good comprehensive evaluation. Cell proliferation experiment showed that mezlocillin at 20 μg/mL had the most significant effect on the proliferation of osteoblasts.Conclusion:The candidate OP therapeutic compound, mezlocillin, was screened from the L1000 data set with good ADMET characteristics and bone cell proliferation promoting activity.
		2022 Vol. 22 (1): 1-7,12 [Abstract] ( 77 ) [HTML 1KB] [ PDF 2191KB] ( 592 )

	8	Treatment of osteoporotic vertebral compression fracture with posterior wall bone defects by percutaneous kyphoplasty with sequential infusion of bone cement
	Treatment of osteoporotic vertebral compression fracture with posterior wall bone defects by percutaneous kyphoplasty with sequential infusion of bone cement[J]. Journal of Jiangsu University(Medicine Edition), 2022,22(1): 8-12>')" href="#">
		Objective:To evaluate the clinical efficacy of the sequential infusion of bone cement when percutaneous kyphoplasty(PKP) was conducted for the treatment of osteoporotic vertebral compression fracture(OVCF) with posterior wall bone defects. Methods: Patients with posterior wall bone defects OVCF treated in our department from May 2016 to July 2020 were retrospectively analyzed. A total of 32 patients meeting the inclusion and exclusion criteria were treated by the sequential infusion of bone cement when PKP was applied. The visual analog score(VAS), Oswestry disability index(ODI), Cobb angle and height of injured vertebra were recorded and compared before surgery and 1 and 3 months after surgery. Postoperative bone cement leakage and complications were also recorded. Results:The postoperative VAS,ODI and Cobb angle of injured vertebra were significantly lower than those before surgery(P＜0.05).The postoperative height of the injured vertebra was significantly higher than that before surgery(P＜0.05). However, there was no significant differences between followup results at 1 and 3 months postoperatively(P＞0.05). There were 3 cases (9.38%) of bone cement leakage, without any cases of spinal canal leakage. No surgical complications such as nerve injury and pulmonary embolism were observed. Conclusion:Sequential infusion of bone cement during PKP is a safe and effective surgery for the OVCF with posterior wall bone defects, which could effectively reduce the bone cement leakage.
		2022 Vol. 22 (1): 8-12 [Abstract] ( 63 ) [HTML 1KB] [ PDF 3856KB] ( 591 )

	13	Network pharmacology of Astragalus membranaceusAtractylodes macrocephala in the treatment of osteoporotic fractures
	Network pharmacology of Astragalus membranaceusAtractylodes macrocephala in the treatment of osteoporotic fractures[J]. Journal of Jiangsu University(Medicine Edition), 2022,22(1): 13-17>')" href="#">	MAO Yinfeng1, CUI Songxiang1, HUA Zhen2, PENG Hongcheng1, WANG Jianwei2
		Objective:To explore the potential mechanism of Astragalus membranaceus and Atractylodes macrocephala in the treatment of osteoporotic fracture (OPF) based on network pharmacology. Methods: The active components and action targets of Astragalus membranaceus and Atractylodes macrocephala were screened by pharmacologic database and Analysis Platform (TCMSP) . GeneCards, OMIM and TTD databases related to disease targets were retrieved to screen the action targets of OPF, and then the intersection core targets of Astragalus membranaceus, Atractylodes macrocephala membranaceus and OPF were screened. Cytoscape 3.7.1 software was used to construct a compoundtargetdisease network, and DAVID database was used to analyze the enrichment of GO function and KEGG pathway. Results: Based on TCMSP database, 19 active components and 115 intersection targets of Astragalus membranaceusAtractylodes for OPF were screened. A total of 568 items were obtained by GO functional enrichment analysis, including 433 biological process items, 43 cell composition items and 92 molecular function items. KEGG pathway enrichment analysis revealed 111 items, including AGE-RAGE, IL-17, TNF, prostate cancer, Toll-like receptor, HIF-1, NF-κB signaling pathway, etc. Conclusion: Astragalus membranaceus and Atelectylodes atelectylodes may regulate inflammatory response and internal balance of osteoclasts by regulating TNF, NF-κB and HIF-1 signaling pathways, and thus exert therapeutic actions on OPF.
		2022 Vol. 22 (1): 13-17 [Abstract] ( 50 ) [HTML 1KB] [ PDF 5686KB] ( 569 )

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		2022 Vol. 22 (1): 18-21,25 [Abstract] ( 59 ) [HTML 1KB] [ PDF 4514KB] ( 539 )

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		2022 Vol. 22 (1): 22-25 [Abstract] ( 41 ) [HTML 1KB] [ PDF 776KB] ( 678 )

	26	Effect of calcium-sensing receptor on the inhibition of proliferation, migration and invasion of hepatocellular carcinoma cell induced by 3,3′-diindolylmethane
	Effect of calcium-sensing receptor on the inhibition of proliferation, migration and invasion of hepatocellular carcinoma cell induced by 3,3′-diindolylmethane[J]. Journal of Jiangsu University(Medicine Edition), 2022,22(1): 26-31>')" href="#">	XU Wei1,2, YANG Qi3, LU Dingkun1, XIAO Zhao1, JIANG Yuanyue1,4, HE Dawei5, SUN Jun6, GU Jie7, YE Yang1, LU Rongzhu1,5
		Objective:To investigate the role of calcium-sensing receptor (CaSR) in the effect of 3,3′diindolylmethane (DIM) on the proliferation, migration and invasion of human hepatocellular carcinoma cells. Methods: Different concentrations of DIM (0, 20, 40, 60 and 80 μmol/L) were used to treat the human hepatocellularcarcinoma cells HepG2 and SMMC-7721 for 24 h. The proliferative ability was detected by MTT assay. Western blotting assay was used to detect the expression of CaSR. The optimal DIM concentration was selected based on the two results above. Transwell assay was performed to detect the ability of migration and invasion. The treatments of cells were divided into four groups: control group, 60 μmol/L DIM group, 300 μmol/L GdCl3 (CaSR agonist) group and 300 μmol/L GdCl3+60 μmol/L DIM co-treatment group. The cells of last group were pretreated with 300 μmol/L GdCl3 for 0.5 h, and then were cotreated with 60 μmol/L DIM for 24 h. The above experiments were repeated to assess the proliferative ability, the expression of CaSR and the ability of migration and invasion of human hepatocellular carcinoma cells. Results: Compared with the control group, after treatment with 60 μmol/L and 40 μmol/L DIM, the proliferative ability of two cell lines was decreased significantly (P<0.05) and the expression of CaSR protein was also markedly decreased (P<0.05); DIM suppressed the ability of migration and invasion of the two cell lines in a concentrationdependent manner. Compared with 60 μmol/L DIM group, the expression of CaSR, cell proliferation, migration and invasion ability were enhanced in the 300 μmol/L GdCl3+60 μmol/L DIM co-treatment group (P<0.05). Conclusion: CaSR plays a role in anti-cancer effect by DIM and may be severed as a potential target for anti-liver cancer.
		2022 Vol. 22 (1): 26-31 [Abstract] ( 59 ) [HTML 1KB] [ PDF 13663KB] ( 498 )

	32	Inhibitory effect of Andrographolide on the metastasis and invasion of human breast cancer cells
	Inhibitory effect of Andrographolide on the metastasis and invasion of human breast cancer cells[J]. Journal of Jiangsu University(Medicine Edition), 2022,22(1): 32-37>')" href="#">	FU Hexiu1, LU Beibei1, CHEN Qiuyun2, GAO Jing1
		Objective:To investigate the effect and mechanism of Andrographolide (Andro) on the metastasis and invasion of human breast cancer MDA-MB-231 cells. Methods: MDA-MB-231 cells were treated with Andro(0,5,10, 20,40 and 80 μmol/L)for 24 h. MTT assay was used to detect cell proliferation.Wound healing and Transwell experiment were applied to detect cell migration and invasion ability.Reverse transcription polymerase chain reaction were applied to detect uncoupling protein 2 (UCP2) mRNA expression.The protein expressions of UCP2 and pyruvate dehydrogenase (PDH) were detected by Western blotting. Lactic acid kit was used to detect lactic acid content. JC-1 staining and laser confocal microscope were used to detect mitochondrial membrane potential (MMP). Results: Compared with the control group, Andro significantly inhibited the proliferation of MDA-MB-231 cells in a concentration-dependent manner. Compared with 0 μmol/L, the metastasis and invasion of MDA-MB-231 cells in the 10, 20, and 40 μmol/L groups were inhibited (P<0.05 or P<0.01); UCP2 mRNA and protein expression were downregulated (P<0.05 or P<0.01), MMP was increased, PDH protein expression was up-regulated, and intracellular lactate content was decreased (P<0.05 or P<0.01). Conclusion: Andro may inhibit the metastasis and invasion of MDA-MB-231 cells by inhibiting the glycolysis of breast cancer cells and altering energy metabolism, which may be related to inhibition of UCP2.
		2022 Vol. 22 (1): 32-37 [Abstract] ( 56 ) [HTML 1KB] [ PDF 21297KB] ( 448 )

	38	ADSC-EVs activate protein kinase B to promote the proliferation and migration and epithelialmesenchymal transition of ovarian cancer cells
	ADSC-EVs activate protein kinase B to promote the proliferation and migration and epithelialmesenchymal transition of ovarian cancer cells[J]. Journal of Jiangsu University(Medicine Edition), 2022,22(1): 38-43,49>')" href="#">	SHEN Nahui1,2, SUN Xiaochun1
		Objective: To explore the effect of extracellular vesicles (EVs) derived from mouse adipose-derived mesenchymal stem cells(ADSCs) on the biological behaviors of ovarian cancer ID8 cell line and its possible mechanism. Methods: ADSCs were isolated by enzyme digestion method, and identified by morphological characteristics, osteogenic and adipogenic multidirectional differentiation ability and flow cytometry surface marker detection; EVs were isolated and purified from supernatant of ADSCs by ultracentrifugation, and ADSC-EVs were identified by transmission electron microscopy, nanoparticle size analysis and Western blotting; ID8 cells were treated with ADSC-EVs at different concentrations (20 μg/mL and 40 μg/mL) and PBS as the control group. The effect of ADSC-EVs on the proliferation of ID8 cells was detected by plate cloning assay. Wound healing and Transwell test were used to detect the effect of ADSC-EVs on the migration ability of ID8 cells. The expression of epithelial-mesenchymal transition(EMT) and protein kinase B(AKT) related proteins in ID8 cells were detected by Western blotting. Results: The isolated ADSCs had typical stem cell morphology, could differentiate into osteogenesis and adipogenesis, and consistent with the general characteristics of the stem cell surface. ADSC-EVs expressed CD9 and CD81 proteins. Compared with the PBS group, the proliferation and migration of ID8 cells in ADSC-EVs group were significantly enhanced (P<0.05), and the proliferation and migration of ID8 cells in 40 μg/mL ADSC-EVS group was stronger than that in 20 μg/mL ADSCEVS group (P<0.05). With the increase of ADSC-EVS concentration, the expression of E-cadherin in ID8 cells was significantly decreased, while the expression of N-cadherin, vimentin and pAkt was greatly increased (P<0.05). Conclusion: ADSC-EVs may promote the EMT of ID8 cells by activating the phosphorylation of AKT signal, thereby enhancing their proliferation and migration ability.
		2022 Vol. 22 (1): 38-43,49 [Abstract] ( 44 ) [HTML 1KB] [ PDF 29531KB] ( 497 )

	44	Repairing effect of mouse bone marrow mesenchymal stem cell-derived extracellular vesicles on nicotineinduced damage of mouse spermatogenic cells
	Repairing effect of mouse bone marrow mesenchymal stem cell-derived extracellular vesicles on nicotineinduced damage of mouse spermatogenic cells[J]. Journal of Jiangsu University(Medicine Edition), 2022,22(1): 44-49>')" href="#">	LIU Ziyao1, HUANG Yue2, WANG Chao1, LI Tao1, WANG Jingzhe1, LIN Bohan1, WANG Zixuan1, SUN Xiaochun1, CHEN Huabiao3
		Objective: To construct an in vitro model of nicotine-induced injury of mouse spermatogenic cells and to explore the repairing effect of mouse bone marrow mesenchymal stem cellderived extracellular vesicles (mBMMSCsEVs) on the nicotine-induced damage of spermatogenic cells GC-1. Methods: Different concentrations (0, 8, 16, 32 μg/mL) of nicotine were used to treat spermatogenic cell GC-1 for 24, 48, 72 h, respectively; and the optimal concentration of nicotine and time of treatment to induce damage on GC-1 cells were screened by MTT assay. The spermatogenic cells GC-1 were divided into control group, nicotine group and nicotine+extracellular vesicle group. MTT, Transwell and apoptosis experiments were used to evaluate the changes in the biological characteristics of damaged GC-1 cell before and after the intervention of mBMMSCsEVs. Results: The results of MTT assay showed that the proliferation of GC-1 was significantly decreased when the concentration of nicotine was 32 μg/mL for 24 h (P<0.05), mBMMSCs-EVS increased cell proliferation after treatment with nicotine (P<0.05); compared with the control group, GC-1 cells in nicotine group showed significantly reduced migration and increased apoptosis(P<0.01); compared with the nicotine group, nicotine+extracellular vesicle group showed significantly increased cell proliferation and migration, and decreased the apoptotic ability(P<0.05 or<0.01). Conclusion: mBMMSCs-EVs may repair the damage of nicotine on the proliferation and migration of spermatogenic cells and inhibited nicotine-induced apoptosis of spermatogenic cells.
		2022 Vol. 22 (1): 44-49 [Abstract] ( 57 ) [HTML 1KB] [ PDF 8445KB] ( 567 )

	50	Expression analysis of lysophosphatidylcholine acyltransferase 1 gene in patients with acute myeloid leukemia
	Expression analysis of lysophosphatidylcholine acyltransferase 1 gene in patients with acute myeloid leukemia[J]. Journal of Jiangsu University(Medicine Edition), 2022,22(1): 50-53>')" href="#">	CHEN Qin1, XU Zijun1, LIN Jiang1, QIAN Jun2, QIAN Wei3
		Objective:To explore the expression status of lysophosphatidylcholine acyltransferase 1 (LPCAT1) gene and its clinical significance in patients with acute myeloid leukemia (AML). Methods:Quantitative realtime PCR was used to examine the LPCAT1 mRNA expression in bone marrow samples from 60 primary AML patients and 27 healthy donors. Results: LPCAT1 mRNA expression was significant lower in AML than controls(P<0.001). LPCAT1 mRNA lowexpression was found in 27 (45%) of 60 AML cases but none in the 27 controls, the difference was statistically significant (χ2=17.618,P<0.001).There was no correlation between the lowexpression of LPCAT1 mRNA with the age, sex, white blood cell count, platelet count, hemoglobin concentration, FAB subtypes and AML karyotypic groups (P>0.05). The receiver operating characteristic (ROC) curve revealed LPCAT1 mRNA expression might serve as a potential diagnostic marker for differentiating AML from controls with an area under the ROC curve of 0.896 (95% CI:0.825~0.967, P<0.001). At the cutoff value of 0.173 of LPCAT1 mRNA expression, the sensitivity and the specificity were 88.3% and 81.5%, respectively.Conclusion: LPCAT1 lowexpression is a frequent molecular event in AML, and it may be used to assist the diagnosis of AML.
		2022 Vol. 22 (1): 50-53 [Abstract] ( 61 ) [HTML 1KB] [ PDF 886KB] ( 490 )

	54	Diagnostic value of serum eotaxin-2 in colorectal cancer patients
		XU Xiaowei, DONG Liyang, ZHENG Tingting, LIU Jiameng, ZHANG Shan, GAO Xuerong, MAO Chaoming
		Objective: To detect the expression of eosinophil chemotactic protein 2 (eotaxin-2) in the serum of patients with colorectal cancer (CRC), and investigate its clinical significance. Methods: A total of 50 CRC patients seeking medical care in the Gastroenterology Department of Affiliated Hospital of Jiangsu University from January 2020 to February 2021 were selected as the CRC group, and 40 healthy subjects in the same period were selected as the healthy controls. The serum eotaxin-2 levels were detected by enzyme-linked immunosorbent assay (ELISA). The association between eotaxin-2 level and the clinicopathological features of CRC was analyzed, and the value of serum eotaxin-2 in the diagnosis of CRC was evaluated by the receiver operating characteristic (ROC) curve. Results: The expression level of serum eotaxin-2 was significantly higher in CRC than that in the healthy controls (Z=-3.93, P<0.01). No significant differences were found among the expression levels of serum eotaxin-2 with respect to the gender, age, lesion location and tumor size (all P>0.05), but was significantly correlated with the lymph-node metastasis, distant metastasis and TNM stage (all P<0.01). The area under the ROC was 0.742 (95% CI: 0.639-0.845, P<0.01). The sensitivity and specificity of serum eotaxin-2 for the diagnosis of CRC were 68.0% and 77.5%, respectively. Conclusion: Serum eotaxin2 level is increased in CRC patients, and may be a candidate biomarker for the CRC diagnosis.
		2022 Vol. 22 (1): 54-57 [Abstract] ( 45 ) [HTML 1KB] [ PDF 1015KB] ( 499 )

	58	Diagnostic value of FibroScan for hepatic fibrosis degree in patients with low levels of alanine aminotransferase hepatitis B
		ZHU Minjia1, XIN Linlin1, DUANMU Wenwen 1, ZHU Jie2, YANG Luxuan3, ZHANG Yingchun4
		Objective:To investigate the diagnostic value of FibroScan in evaluating the degree of liver fibrosis in chronic hepatitis B patients with hepatitis B virus DNA>20 000 IU/mL but alanine aminotransferase (ALT) less than or equal to 2 times of the upper limit of normal. Methods:A total of 57 chronic hepatitis B patients with hepatitis B virus DNA>20 000 IU/mL but ALT less than or equal to 2 times of normal upper limit were selected from January 2018 to August 2019 in the Department of Hepatology of The Fifth People′s Hospital of Suzhou. At the same time, FibroScan, acoustic pulsed radiation force imaging (ARFI) and blood biochemical indexes were determined to calculate aspartic aminotransferase to platelet ratio index (APRI). With the pathological results of liver tissue as the gold standard, ROC curve analysis was performed to evaluate the value of FibroScan, ARFI and APRI in noninvasive evaluation of hepatitis B fibrosis. Results:The area under ROC curve (AUC) of FibroScan, ARFI and APRI were 0.88,0.89,0.98,0.86,0.82,0.89 and 0.69,0.70,0.73 when liver fibrosis stage ≥S2, ≥S3 and S4, respectively. Pairwise comparison results showed that there was no statistical difference between the AUC of each stage assessed by FibroScan and the ARFI of the same period. The AUC of each stage assessed by FibroScan was higher than those in the APRI of the same period (Z=2.26, P=0.01; Z=2.33, P=0.01; Z=2.84, P<0.001). The Youden index (sensitivity+specificity-1) of FibroScan, ARFI and APRI were 63.44%, 67.77% and 33.89%, respectively, when evaluating hepatitis B fibrosis ≥S2. the Youden index of FibroScan, ARFI and APRI were 65.79%, 63.16% and 31.57%, respectively, when evaluating hepatitis B fibrosis ≥S3. In the evaluation of hepatitis B fibrosis in S4, FibroScan′s Youden index was 93.75%, much higher than ARFI′s 72.22% and APRI′s 45.14%, and the sensitivity reached 100%. Conclusion:For chronic hepatitis B patients with hepatitis B virus DNA>20 000 IU/mL but ALT less than or equal to 2 times of the upper limit of normal, the comprehensive value of FibroScan in evaluating the severity of liver fibrosis is better than that of ARFI and APRI, especially when evaluating S4, which can be used as a basis for judging the acceptance of antiviral therapy.
		2022 Vol. 22 (1): 58-62 [Abstract] ( 64 ) [HTML 1KB] [ PDF 5600KB] ( 521 )

	63	Effect of oxycodone combined with levobupivacaine on epidural labor analgesia
	Effect of oxycodone combined with levobupivacaine on epidural labor analgesia[J]. Journal of Jiangsu University(Medicine Edition), 2022,22(1): 63-66,71>')" href="#">	ZHAO Jing, HUO Qifan, ZHANG Shiping, SONG Yulong
		Objective: To assess the effect of oxycodone combined with levobupivacaine on epidural analgesia for labor. Methods: A total of 84 parturients who were to receive epidural labor analgesia were divided into two groups randomly, with 42 patients in each group. The Group oxycodone received 0.1% levobupivacaine with 0.2 mg/mL oxycodone, and the Group fentanil received 0.1% levobupivacaine with 2 μg/mL fentanil for epidural labor analgesia. The visual analogue scale/score(VAS), Bromage score, the onset time of analgesia, the first time of patientcontrolled epidural analgesia(PCEA), labor duration, delivery mode, adverse reactions, Apgar score and umbilical cord blood gas analysis at each time point were observed. Results: The VAS at each time point in the two groups was lower than that before analgesia (P<0.05). There was no significant difference in the analgesia score at each time point between the two groups. The onset time of analgesia was shorter in Group oxycodone than that in Group fentanil (P<0.05). The first time of PCEA was longer in Group oxycodone than that in Group fentanil (P<0.05). There was no significant difference in Bromage score, labor duration, delivery mode, adverse reactions, Apgar score and umbilical cord blood gas between the two groups(P>0.05). Conclusion: Levobupivacaine combined with oxycodone could accelerate the onset time of analgesia and prolong the first time of PCEA. This combination contributes steady labor duration without increasing complications during labor. It is a safe and feasible strategy for labor analgesia.
		2022 Vol. 22 (1): 63-66,71 [Abstract] ( 54 ) [HTML 1KB] [ PDF 805KB] ( 555 )

	67	Effect of lidocaine on autonomic nervous function during pneumoperitoneum in gynecological laparoscopic surgery
		LI Feifei, LIU Fengxia, YANG Dawei, GUO Miao, ZHANG Jianyou
		Objective: To investigate the effect of intravenous lidocaine on autonomic nervous function during pneumoperitoneum in gynecological laparoscopic surgery. Methods: A total of 70 patients with laparoscopic gynecological surgery under general anesthesia were divided into two groups by random number table. In the lidocaine group 1.5 mg/kg of lidocaine was intravenously administered during induction and maintained at 1.5 mg/(kg·h) during surgery. The control group received the same amount of normal saline. A 5min Holter electrocardiogram was collected for heart rate variability analysis to evaluate autonomic function after admission (T0), intubation (T1), immediately after pneumoperitoneum formation (T2), 10 min after pneumoperitoneum formation (T3), 20 min after pneumoperitoneum formation (T4), 30 min after pneumoperitoneum formation (T5), and extubation (T6). Intraoperative blood pressure and heart rate were also recorded. Results: Compared with control group, the sympathetic related index of the ratio of low to high frequency band powers (LF/HF) and the normalized unit of highfrequency band power (LFnu) was lower (P<0.05) while the parasympathetic related index the normalized unit of highfrequency band power (HFnu) was higher (P<0.05) in the lidocaine group than that in the control group from T1 to T6. The logtransformed measure of totalfrequency band power (lnTP), the logtransformed measure of lowfrequency band power (lnLF) and the standard deviation of the interbeatinterval of normal sinus beats (SDNN) from T3 to T5 were lower than those in control group (P<0.05). In the lidocaine group, the mean arterial pressure was lower from T1 to T6 than that in control group (P<0.05). Conclusion: Intravenous lidocaine infusion in gynecological laparoscopic surgery reduces sympathetic excitability during pneumoperitoneum and improves the status of autonomic nervous function.
		2022 Vol. 22 (1): 67-71 [Abstract] ( 63 ) [HTML 1KB] [ PDF 1030KB] ( 559 )

	72	Preparation of zingerone nano-mixed micelles and its regulating effect on hyperlipidemia
	Preparation of zingerone nano-mixed micelles and its regulating effect on hyperlipidemia[J]. Journal of Jiangsu University(Medicine Edition), 2022,22(1): 72-79>')" href="#">	GE Zhumei, YU Jiangnan, XU Ximing
		Objective: To evaluate the effect of zingeroneloaded nanomixed micelles on hyperlipidemia. Methods: The thin film dispersion method was used to prepare zingerone nanomixed micelles（ZNMs）. The prescription was optimized and screened through single factor and orthogonal experiments, and the apparent morphology, particle size, drug loading and encapsulation efficiency were determined. Oleic acid was used to induce lipids in human liver cancer cells and high-fat diets were used to establish a highfat model to analyze the effect of ZNMs on the regulation of hyperlipemia. Results: The optimal prescription for ZNMs is 40 mg, phospholipid ∶sodium cholate 2 ∶3（m/m）, polyvinylpyrrolidone K3（PVP K30） 30 mg, and vitamin E polyethylene glycol succinate（TPGS） 22 mg. The particle size of the prepared ZNMs is （50.62±0.25）nm, and the encapsulation efficiency is （94.71±2.02）%. In vitro release experiments showed that the cumulative release rate of ZNMs is significantly higher than that of the bulk drug. ZNMs could reduce blood lipid metabolism in hyperlipidemia mice caused by highfat diet, and could also decrease cell lipid accumulation induced by oleic acid. Compared with the zingerone group, the blood lipid level of ZNMs group was significantly improved（P<0.05）. Conclusion: Nanomixed micelles enhance solubilization of zingerone, improve the antihyperlipidemia efficacy, and effectively come into effect in the whole organism.
		2022 Vol. 22 (1): 72-79 [Abstract] ( 50 ) [HTML 1KB] [ PDF 157416KB] ( 482 )

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		2022 Vol. 22 (1): 80-83 [Abstract] ( 46 ) [HTML 1KB] [ PDF 1474KB] ( 652 )

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		2022 Vol. 22 (1): 84-87,92 [Abstract] ( 41 ) [HTML 1KB] [ PDF 812KB] ( 656 )

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		2022 Vol. 22 (1): 88-92 [Abstract] ( 50 ) [HTML 1KB] [ PDF 791KB] ( 543 )

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