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Journal of Jiangsu University(Medicine Edition)
 
2015 Vol.25 Issue.02
Published 2015-03-30
Article
93 Ionizing radiation strengthened EMT and metastasis in esophageal squamous carcinoma cells
Objective: To detect the progress of epithelialmesenchymal transition(EMT) in esophageal squamous carcinoma cells(ESCC)TE1 which was treated with ionizing radiation, and explore the underlying mechanisms. Methods: After treated with different intensity of ionizing radiation(0, 2, 4, 8 Gy),realtime PCR was used to detect the mRNA expression of Snail 1 and AP1 in TE1 cells. Western blot was used to measure the protein expressions of Snail 1, Slug, Ecadherin and Vimentin. The protein levels of Ecadherin and Vimentin was verified by immunofluorescence. The ability of migration was measured by wound healing test and Transwell inserts. Dealing with ionizing radiation(4 Gy) and/or DAPT the inhibitor of Notch signaling pathway, the expression levels of Notch 1, NICD, Hes 1 and MMP9 proteins were measured by Western blot. Results: After treated with ionizing radiation, TE1 cells showed the upregulation effect of the transcription factors of EMT(Snail 1, Slug, AP1), mesenchymal marker(Vimentin) and metastasis related protein (MMP9), meanwhile the downregulation effect of epithelial marker( Ecadherin).and when dealing with 4 Gy radiation, the changes was significant (P<0.01). Ionizing radiation accelerated the transfer ability of TE1（P<0.05）. By upregulating the expression levels of Notch 1/NICD/Hes 1 signaling pathway, ionizing radiation promoted EMT related protein expressions(P<0.01). Conclusion: The ionizing radiation strengthens EMT, thereby induces metastasis, by activating Notch signaling pathway.
2015 Vol. 25 (02): 93-97 [Abstract] ( 784 ) [HTML 1KB] [ PDF 1290KB] ( 1849 )
98 Preparation and identification of doxycycline polyclonal antibody
WANG Jun1,2， YIN Lei1, JIANG Yanqiu1,3， ZHUO Ya1,4， LIU Xia1, WANG Hui1， ZHANG Miaomiao1, DU Fengyi1, XIA Sheng1, SHAO Qixiang1
Objective: To conjugate the doxycycline (Dox) with bull serum albumin (BSA)/ovalbumin (OVA) and generate polyclonal antibody against the Dox. Methods: The Dox was coupled with BSA/OVA via direct, onestep formaldehyde, diazo plus mixed anhydride and diazo plus carbodiimide conjugation method, respectively. The BALB/c mice were immunized with DoxBSA which conjugated by most efficient method. The titer, sensitivity and specificity of antibody were detected and assayed by direct or indirect competitive inhibition ELISA. Results: It had been declared that the diazo plus carbodiimide conjugation method was most efficient, and the coupling ratio of Dox ∶BSA was 8.37 ∶1, and Dox ∶OVA was 4.92 ∶1. The polyclone antibody (pAb) titer was more than 1 ∶8 000 by indirect ELISA. The half maximal inhibitory concentration (IC50) was 98.89-120.32 μg/L. There was less the cross reactivity with other tetracyclines. Conclusion: The conjugation method of diazo plus carbodiimide was the most efficient and the pAb has high titer, better sensitivity and specificity.
2015 Vol. 25 (02): 98-102 [Abstract] ( 1108 ) [HTML 1KB] [ PDF 1683KB] ( 1720 )
103 Knockdown of RAD18 gene by siRNA enhance the sensitivity to cisplatin in lung adenocarcinoma
SU Jin-yu1, Li Jian1, CHEN Yong-chang2, WU Min2
Objective: To investigate the changes of the FA/BRCA pathway DNA repair function, as well as the sensitivity to cisplatin in human lung adenocarcinoma A549 cells and A549/DDP cell lines (a cisplatinresistance A549 cell line) after knockdown of the E3 ubiquitin ligase RAD18 gene by siRNA technique. Methods: Using RNA interfering approach to transfect RAD18siRNA into A549 and A549/DDP cells by Lipofectamine. Western blotting was carried out to test the expression of RAD 18 protein, and the FANCD2 monoubiquitination levels, as indicated by the ratio of FANCD2L (long form of FANCD2) to FANCD2S (short form of FANCD2) in the two cells treated with cisplatin before and after RAD18siRNA transfection. CCK8 technique was used to detect the cell proliferation rate of the two cells treated with cisplatin preandpost transfection of RAD18siRNA. Immunofluorescence assay was performed to inspect the formation of FANCD2 nuclear foci. Flow cytometry using Annexin V/PI methods was conducted to measure cell apoptosis rate. Results: After transfection of RAD18siRNA,the expression of RAD18 protein,and the levels of FANCD2 monoubiquitination(L/S ratio), and the nuclear foci formation of FANCD2 had a significant decline in A549 and A549/DDP cells treated with cisplatin compared with before transfection. Meanwhile, we found that knockdown of RAD 18 gene can suppress the proliferation rate and promote apoptosis induced by cisplatin in the two cells (both P<0.05). Conclusion: Knockdown of RAD18 gene by RNA interfering approach can potentiate the sensitivity to cisplatin in A549 and A549/DDP cells through inhibition of FA/BRCA pathway DNA damage repair function.
2015 Vol. 25 (02): 103-108 [Abstract] ( 962 ) [HTML 1KB] [ PDF 1412KB] ( 1900 )
109 Effect of ramatroban on podocyte apoptosis induced by puromycin aminonucleoside
ZHU Xiao-dong1，2， KONG Cheng1， KONG Wei-xin3， LU Zhi-feng4， ZOU Feng4
Objective: To observe the ultrastructure changes of ramatroban on podocyte apoptosis induced by puromycin aminonucleoside (PAN). Methods: The conditionally immortalized murine podocytes were randomly divided into four groups: control group, the podocytes were incubated with RPMI1640; model group, the podocytes exposed to 100 mg/mL PAN; treatment group, the podocytes were pretreated with 100 μg/mL ramatroban for 1 h before 100 mg/mL PAN exposure; ramatroban group, podocytes exposed to 100 μg/mL ramatroban. Each group cells were collected at 24 h and 48 h, respectively. The level of podocytes apoptosis was detected by FITCAnnexin V/PI assay under fluorescence microscope. The ultrastructure changes of podocyte morphology were observed with transmission electron microscope. Results: Compared with control group, the apoptosis was significantly increased in model group and treatment group (P0.05), while the apoptosis of treatment group was obviously decreased at 24 h compared with model group (P<0.05), the apoptosis in treatment group at 48 h was significantly increased than control group at 48 h. Compared with model group, the cell ultrastructural damage in treatment group was significantly reduced after treatment with ramatroban at 24 and 48 h; TEM displayed cytoplasmic vacuolar degeneration and apoptotic bodies obviously decreased. Conclusion: Ramatroban could reduce PANinduced podocyte apoptosis rate and cell injury.
2015 Vol. 25 (02): 109-113 [Abstract] ( 1081 ) [HTML 1KB] [ PDF 1118KB] ( 1622 )
114 Role of the primary sensory neurons PKA/CREB signaling pathway in bone cancer pain rats
WANG Feng1, LI Meng2, WEI Jing-rong2, LU Xiang-rong1, ZHOU You-lang2, XU Wei-yuan3
2015 Vol. 25 (02): 114-117 [Abstract] ( 865 ) [HTML 1KB] [ PDF 988KB] ( 1551 )
119 Effect of nicotinic acetylcholine receptors on the growth and apoptosis of human lung adenocarcinoma A549 cell line
SU Chun-xiang1,2, LIU Sha1, JIN Hui1,2, WANG Mu-bin1, LIU Yang2, ZHANG Jin2, YAN Yu-lan2
Objective: To explore the effect of nicotinic acetylcholine receptor (α7nAChR) agonist and antagonist on the growth and apoptosis of lung adenocarcinoma A549 cell line. Methods: Human lung adenocarcinoma cell line A549 was divided randomly into control group, agonist group and antagonist group, A549 cell line was infected with PBS, agonist, and antagonist following 24,48 and 72 h, respectively. And the optimum concentration and time of nAChR agonist and antagonist were selected by MTT; the expression of Caspase9, Bax and Bcl2 were detected by Western blotting, α7nAChR expression was determined by realtime PCR and cell apoptosis was examined by Tunel method. Results: A549 cells stably expressed α7 nAChR. 10-3 mol/L and 48 h were the optimum concentration and time for nAChR agonist and antagonist. At the concentration(10-3 mol/L) and time (48 h), compared with control group, α7nAChR in antagonist group was decreased, while increased in agonist group; but the expression of Caspase9 and Bax/Bcl2 and cell apoptosis levels were increased in antagonist group (P<0.01). Conclusion: α7nAChR agonist and antagonist was closely related to the growth and apoptosis of lung adenocarcinoma A549 cell line, respectively, the cell apoptosis of which might correlated with activation of Bax/Bcl2 and Caspase family induced by α7nAChR antagonist.
2015 Vol. 25 (02): 119-122 [Abstract] ( 1026 ) [HTML 1KB] [ PDF 1321KB] ( 1811 )
123 Construction of human PDL1 gene transfected cell line and its biological function on the proliferation and apoptosis of activated Jurkat cell
SONG Li1, GE Yan1, CAO Sha-sha1, XU Yong-fang1, PAN Jian-zhong1, XIE Wei1, JU Song-wen2, JU Song-guang1
Objective: To construct a gene transfected cell line that stably expressing the human PDL1 and observe its biological function on the proliferation and apoptosis of activated Jurkat cell. Methods: Human PDL1 gene was subcloned into retroviral expressing vector pEGZTermR. The recombinant plasmid together with its helper virus vector was cotransfected into the 293T cells. The L929 cells were infected with the supernatant of the transfected 293T cells, and then were selected with G418. The G418 resistant cells were harvested for screening PDL1 expression by flow cytometry. L929/PDL1 cell was cocultivated with PHA stimulated Jurkat cell. The biological effect on proliferation and apoptosis of Jurkat cell was analyzed by cell counting and flow cytometry. Results: Gene transfected line L929/PDL1 that stably expressed the human PDL1 was established. Membrane PD1 expression on Jurkat cell was induced by PHA administration. L929/PDL1 inhibited the proliferation and induced the apoptosis of activated Jurkat cell. Conclusion: Gene transfected cell line that stably expressed the human PDL1 was established successfully.The PDL1 on the cell line inhibited the proliferation and induced the apoptosis of activated Jurkat cell.
2015 Vol. 25 (02): 123-127 [Abstract] ( 1624 ) [HTML 1KB] [ PDF 1439KB] ( 1668 )
128 Toll-like receptor 4 and knee osteoarthritis chondrocytes apoptosis
QIAN Chen1， BIAN Xiao-xing2
Objective: Discuss the relationship between human knee osteoarthritis chondrocytes apoptosis and Tolllike receptor 4 (TLR4) expression, to see whether TLR4 signaling pathways effect or not on pathogenesis of osteoarthritis. Methods: Jonit cartilage was shredded after harvested from the patients of osteoarthritis undergoing the replacements of knee, and chondrocytes were isolated and cultured by the way of enzymatic digestion. The thirdpassage cells in the logarithmic growth phase were cultured in vitro and randomly divided into ZVADFMK (ZVF) group,camptothecin group,control group. TUNEL method was used to assay apoptosis index of the chondrocytes. Immunohistochemical staining and Western blotting were adopted to observe TLR4 expression.The chondrocytes were randomly divided into control group and TLR4 inhibitor group. Flow cytometry instrument and Caspase3 kit were used to assay apoptosis index of the chondrocytes. Results: The results of TLR4 immunohistochemical staining and Western blotting were that the expression of TLR4 was induced by camptothecin but reduced by ZVF ,in parallel with the results of cells apoptosis index. In addition,chondrocyte apoptosis was reduced by incubation with TLR4inhibitor. The results of Flow cytometry instrument and Caspase3 kit were consistent. Conclusion: Apoptosis chondrocytes could upregulate TLR4 expression. There was a positive correlation between TLR4 and apoptosis.TLR4inhibitor could reduce chondrocytes apoptosis in vitro model. TLR4 signaling pathways involved in chondrocytes apoptosis and played an important role on osteoarthritis.
2015 Vol. 25 (02): 128-132 [Abstract] ( 1155 ) [HTML 1KB] [ PDF 1512KB] ( 1836 )
133 Inhibition effect of asiatic acid on the proliferation and autophagy of HCT116 colon cancer cell
GENG Ji, GUO Wen-jie, LIU Jia, TAN De-fei, DANIEL Boison, GAO Jing
Objective: To investigate the effect of asiatic acid on the proliferation and autophagy level in HCT116 cells. Methods: After treating HCT116 cells with 10,30,60,90 μmol/L asiatic acid for 24 h and 48 h, the cell viability ratio was detected by MTT method. The formation of autophagy vesicles were observed by MDC autophagy specific fluorescence staining. The expressions of LC3Ⅱ, p62,pmTOR,p4EBP1 were detected by Western blotting. Results: Asiatic acid inhibited the proliferation of HCT116 when they were treated with 90 μmol/L. Autophagy was induced by 30,60,90 μmol/L of asiatic acid and the autophagic marker LC3Ⅱ was strongly expressed at 90 μmol/L asiatic acid treatment for 4,8,12 h as shown by Western blotting. A combination of 5 μmol/L chloroquine with 90 μmol/L of asiatic acid,promote autophagic flux in a dosedependent manner. Conclusion: Asiatic acid can inhibit the proliferation of HCT116 cells obviously and promote autophagic flux by inhibiting mTOR4EBP1 signal pathway in a time and dosedependent manner.
2015 Vol. 25 (02): 133-136 [Abstract] ( 1291 ) [HTML 1KB] [ PDF 1132KB] ( 1671 )
137 Inhibition expression of miR31 suppresses pancreatic cancer panc1 cell line migration and invasion and potential mechanisms
CAO Dan1, HU Bo2, HUANG Feng-bo1, ZHU Min-sheng1, SUN Mao-min3, KUANG Yu-ting4, WANG Shou-li1
Objective: To investigate the regulating mechanisms of miR31 in Panc1 cells for the purpose of providing a theoretical basis for further research. Methods: miR31 inhibitory sequences was analysed by software to select targeted interference sites. The miR31 inhibitory sequences with different amount was transfected into Panc1.Transfect efficiency was assessed by Live cell Imaging System and realtime RTPCR. miR31 inhibitory sequences was transfected into Panc1 cells, paralleled with blank and negative control groups. Invasion and migration were assayed by Transwell; target genes were predicted by bioinformatics way；the expression level of target proteins were detected by Western blotting. Results: The maximal transfection efficiency occurred when the amount of miR31 inhibitory sequences was 5 μL,the efficiency was up to 90.6%.The results of realtime RTPCR reveled that the expression level of miR31 in the experiment group is decreased about 5 times than control group(P<0.05);inhibition expression of miR31 could effectively inhibit the migration (P<0.05) and invasion (P<0.05)of Panc1 cells; bioinformatics predicted three target genes: PRKCε、RhoBTB1 and SATB2;the results of Western blotting revealed that the levels of RhoBTB1 was increased most significantly. Conclusion: Inhibition expression of miR31 could inhibit the abilities of invasion and migration in Panc1 cells; the expression of RhoBTB1 may play an important role at the mechanism of miR31 in Panc1 cells.
2015 Vol. 25 (02): 137-141 [Abstract] ( 1232 ) [HTML 1KB] [ PDF 1202KB] ( 1408 )
142 Expression of Ki67, MMP9 before and after neoadjuvant chemotherapy in breast cancer and its clinical significance
ZHANG Yi-jun1, CUI Da-peng2, WU Xue-liang3, LI Kun4, WANG Li-kun5, XIN Guo-feng1, LU Yu6, LI Qing6
Objective: To explore the expression of Ki67 and MMP9 before and after the neoadjuvant chemotherapy (NCT) in breast cancer and the relationship between the changes in biological marker expression and response to neoadjuvant chemotherapy. Methods: One hundred and thirty patients with stage Ⅲ invasive breast cancer were selected in the study. All patients received 6 cycles NCT, docetaxel, epirubicin, and cyclophosphamide, and the efficacy were evaluated. The expression of Ki67 and MMP9 in the tissues before NCT and after operation were examined by immunohistochemistry. Results: ① The response of NCT in breast cancer patients: 26 patients (20.97%) showed clinical complete response; 66 patients (53.23%) showed partial response; 28 patients (22.58%) showed stable disease; 4 patients (3.22%) showed progressive disease；the clinical efficiency was 74.19% (92/124), the clinical benefit rate was 96.77% (120/124); there were 24 patients (19.35%) got pathological complete response；② Compared the efficiency of NCT in breast cancer between the patients with Ki67 positive(72 cases) and those with Ki67 negative(52 cases) before and after operation, the difference showed statistically significant(χ2=6.368, P=0.012); compared the efficiency of NCT in breast cancer between the patients with MMP9 positive(80 cases)and those with MMP9 negative(44 cases) before and after operation, the difference showed statistical significance(χ2=4.062, P=0.044); ③ After NCT, the Ki67 index and the MMP9 expression levels were significantly decreased (t=3.136, P=0.003; χ2=8.911, P=0.030). Conclusion: After NCT, expressions of Ki67 and MMP9 were greatly reduced, which might be used as the biological factor for predicting the efficacy of NCT in breast cancer.
2015 Vol. 25 (02): 142-145 [Abstract] ( 969 ) [HTML 1KB] [ PDF 991KB] ( 1692 )
146 Clinical effect of treatment with the guidance of the fractional flow reserve in coronary artery bifurcation lesion
ZHANG Xiao-yong1, WANG Zhao-jun2, ZHOU Lei1, LV Yuan1
Objective: To study the value of fractional flow reserve (FFR) for critical lesion of coronary bifurcation in percutaneous coronary intervention (PCI). Methods: A total of 80 inpatients with bifurcation critical lesion of coronary were investigated. The matched patients were divided into FFR guiding group (FFR group) and conventional PCI group, 40 cases for each group. The data including basic clinical characteristics, results of PCI, and main adverse cardiovascular events (MACE) during followup were analyzed retrospectively. Results: There were significantly difference in sidebranch lesion stent implanted after PCI, the ratio of kissing balloon, and the remanent stenosis (>50 percent in diameter) of sidebranch between the FFR group and the conventional PCI group (P was 0.026, 0.021, and 0.043, respectively). The total ratio of MACE between two groups had no statistic difference at sixmonth and 18 months followup (P>0.05). However, the incidence of MACE after 3 years of followup was higher in conventional PCI group than that of FFR group(P<0.05). Conclusion: FFR for bifurcation critical lesion of coronary may reduce unnecessary application of kissing balloon or stenting in sidebranch of bifurcation, and reduce the occurrence of MACE after PCI in following up.
2015 Vol. 25 (02): 146-148 [Abstract] ( 824 ) [HTML 1KB] [ PDF 751KB] ( 1319 )
149 Evaluation of plasma miR-197-3p and miR3613p expression for the diagnosis of lung cancer
ZHU Li-huan1, LI Jian1, ZHU Yu-min1, YU Li-chao2, WANG Yi3
Objective: To investigate the clinical value of plasma miR1973p and miR3613p expression in diagnosing lung cancer. Methods: A realtime quantitative PCR method was used to measure the levels of plasma miR1973p and miR3613p in 57 patients with lung cancer and 53 patients with benign lung disease. Serum carcinoembryonic antigen (CEA) and cytokeratin 19 fragment(Cyfra211) were also determined simultaneously. The receiver operating characteristic(ROC) curves were constructed to assess the diagnostic performance of the two miRs. The relations between the plasma two miRs levels and patient clinicopathological characteristics were analyzed. Results: The plasma levels of miR1973p and miR3613p in lung cancer patients were significantly higher than those in benign lung disease patients(P＜0.001).The areas under ROC curves of miR1973p and miR3613p were 0.908 and 0.869 respectively for the diagnosis of lung cancer(both P＜0.001). Plasma miR1973p expression was significantly associated with histology type (P＝0.011). Whereas plasma miR3613p expression was correlated with tumor differential degree(P＝0.005) and clinical stage(P＝0.015). For the diagnosis of lung cancer, plasma miR1973p and miR3613p exhibited the higher sensitivity(78.9% and 71.9%) and accuracy (81.8% and 80%), as compared with serum CEA(42.6% for sensitivity and 60% for accuracy) and Cyfra211(25% for sensitivity and 58.9% for accuracy). Combination of miR1973p and miR3613p raised sensitivity and accuracy to 89.5% and 85.5%, respectively, with a slight decrease of specificity. Conclusion: Plasma miR1973p and miR3613p were superior to serum CEA and Cyfra211 as tumor markers in lung cancer diagnosis.
2015 Vol. 25 (02): 149-154 [Abstract] ( 1429 ) [HTML 1KB] [ PDF 796KB] ( 1829 )
155 Expression and clinical significance of Pololike kinase 1 in cervical squamous cell carcinoma
GE Hai-Yun, LIU Biao,WU Jin-chang, ZHOU Jun-dong
Objective: To investigate the expression of Pololike kinase 1 (PLK1) in cervical squamous cell carcinoma (SQCC) and its clinical significance. Methods: The immunohistochemical staining method was conducted to detect the expression of PLK1 in 34 cases of cervical squamous epithelium, 82 cases of cervical SQCC, 166 cases of cervical intraepithelial neoplasia (CIN), including 48 cases of CIN Ⅰ, 42 cases of CIN Ⅱ and 76 cases of CIN Ⅲ/carcinoma in situ. And the correlation of PLK1 expression and clinicopathologic factors of cervical SQCC was analyzed. Results: There was significant difference in the expression rate of PLK1 among cervical squamous epithelium, CIN Ⅰ, CIN Ⅱ, CIN Ⅲ/carcinoma in situ and cervical SQCC, which was 14.71%, 22.92%, 35.71%, 69.74% and 86.59%,respectively(χ2=87.42，P0.05). In cervical SQCC, there was significant difference in the survival curves between the patients with high PLK1 expression and those with low or negative PLK1 expression(P<0.05). Conclusion: The PLK1 overexpression might involve in the development, invasion and metastasis and could be used as a prognostic factor of cervical SQCC.
2015 Vol. 25 (02): 155-158 [Abstract] ( 810 ) [HTML 1KB] [ PDF 779KB] ( 1597 )
159 Prevention of intensive statins therapy before percutaneous coronary intervention in myocardial noreflow phenomenon : a metaanalysis
ZHONG Jian1, FU Qiang2， ZHU Ke2， ZHANG Qian2
Objective: To systematically evaluate the prevention of intensive statins therapy and standard statins therapy before percutaneous coronary intervention for acute coronary syndrome patients in noreflow phenomenon of myocardium. Methods: PubMed, Medline, Science, Embase, Cochrane Library, CNKI, Wanfang and VIP were searched to collect randomized controlled trials(RCTs) of prevention of intensive statins therapy before percutaneous coronary intervention in noreflow phenomenon of myocardium.Retrial time was from the inception of those database to July,2014.Jadad score were used for literature evaluation and information extraction. Metaanalysis was performed by RevMan 5.3 software. Results: Totally six RCTs that contained 855 acute coronary syndrome patients with percutaneous coronary intervention were enrolled, in which the group of intensive statins therapy had 429 cases and the group of standard statins therapy had 426 cases. Metaanalysis showed that the rate of noreflow significantly decreased in the intensive statins therapy cases compared with that of the standard statin therapy cases undergoing percutaneous coronary intervention\[9.56%vs 26.06%,OR=0.28,95%CI(0.19,0.41)\]. Conclusion: The intensive statins therapy before percutaneous coronary intervention can significantly reduce the incidence of noreflow in acute coronary syndrome patients during operation compared with standard statins therapy.
2015 Vol. 25 (02): 159-163 [Abstract] ( 894 ) [HTML 1KB] [ PDF 853KB] ( 1474 )
164 Correlation of polymorphisms of p53 and MDM2 genes with severe hematologic toxicity in patients with lung adenocarcinoma
CHENG Xiao-qing1, JIANG Xiu-qin2
Objective: To investigate the association of p53 p.Pro72Arg and MDM2 c.14+309T>G polymorphisms with treatmentrelated hematologic toxicity in patients with advanced lung adenocarcinoma. Methods: The polymorphisms were genotyped by the polymerase chain reactionrestriction fragment length polymorphism (PCRRFLP) in the 267 patients with advanced lung adenocarcinoma. Results: p53 p.Pro72Arg was not correlated with incidence of severe hematologic toxicity in the advanced patients treated with platinumbased chemotherapy. The MDM2 c.14+309T>G T allele was associated with increased hematologic toxicity risk following platinumbased chemotherapy treatment in advanced adenocarcinoma patients. The stratification analysis showed the MDM2 c.14+309T>G was significantly associated with severe hematologic toxicity in the patients below 70 years or with stage Ⅳ adenocarcinoma in recessive model (adjusted OR=2.254, 95%CI=1.069-4.754, P=0.033 and adjusted OR=2.351, 95%CI=1.023-5.401, P=0.044, respectively). Conclusion: MDM2 c.14+309T>G may be used as one of the candidate biomarkers for predicting severe hematologic toxicity in advanced adenocarcinoma patients who had platinumbased chemotherapy treatment.
2015 Vol. 25 (02): 164-168 [Abstract] ( 898 ) [HTML 1KB] [ PDF 1404KB] ( 1795 )
169 Expression of BAALC and its clinical significance in patients with acute myeloid leukemia
YANG Hai-yan, ZHOU Jing-dong, YANG Lei, JI Yong-hui, ZHANG Ming, QIAN Jun
Objective: To investigate the expression of BAALC (brain and acute leukemia, cytoplasmic) gene in acute myeloid leukemia (AML) and to analyze its clinical significance. Methods: Bone marrow from 93 AML patients (research group) and 36 healthy volunteers (control group) were collected for the study. Realtime quantitative PCR (RQPCR) was used to detect BAALC expression level in the bone marrow mononuclear cells. Clinical significance of BAALC expression was compared in two groups divided by ROC curve analysis according to BAALC mRNA level. Results: BAALC level in AML patients was significantly higher than control group (P<0.001). AML patients were divided into two groups (high BAALC expression and low BAALC expression) according to the cutoff value of 2.107 (51% sensitivity and 100% specificity, respectively). No significances were found in the sex, age, blood parameters between the two groups except for WHO classifications, FAB subtypes and cytogenetic risks. High BAALC expression group patients had significantly higher blasts compared to low BAALC expression group patients (P<0.001). High BAALC expressed patients had a trend towards lower complete remission (CR) than low BAALC expressed patients (P=0.087). AML patients with high BAALC expression had significantly shorter overall survival (OS) than those with low BAALC expression (P=0.031). Among AML patients with normal cytogenetics, high BAALC expression patients also showed significantly shorter OS than those with low BAALC expression (P=0.043). Conclusion: Overexpression of BAALC may act as a potential biomarker contributing to the diagnosis of AML, and may predict an adverse prognosis in AML.
2015 Vol. 25 (02): 169-174 [Abstract] ( 968 ) [HTML 1KB] [ PDF 1105KB] ( 1676 )
175 Expression of early growth response protein 2 in sciatic nerve of diabetic neuropathy rat
ZHANG Hua1， YIN Juan2， HE Xue-qin1， LIU Xing-hui3
Objective: To investigate the expression of early growth response protein 2(EGR2) in diabetic neuropathy rat. Methods: Six as a control group from 60 rats, the other 54 rats celiac injection of streptozotocin, 5 of 23 diabetic rats formed neuropathy. Rats sciatic nerve conduction velocity and tail vein blood glucose were detected, each took a small section of sciatic nerve to have crosssectional electron microscopy (sem) examination. PCR and western blot were used to detect the expression of EGR2. Results: The results of EGR2 mRNA and protein illustrated that EGR2 expression was higher in diabetic neuropathy rat. Conclusion: The expression of EGR2 in diabetic neuropathy was obviously higher in diabetic neuropathy rat, which may be through the IL6/gp130/STAT3 pathway.
2015 Vol. 25 (02): 175-178 [Abstract] ( 985 ) [HTML 1KB] [ PDF 1396KB] ( 1749 )
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2015 Vol. 25 (02): 179-181 [Abstract] ( 648 ) [HTML 1KB] [ PDF 748KB] ( 1774 )
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2015 Vol. 25 (02): 182-184 [Abstract] ( 583 ) [HTML 1KB] [ PDF 719KB] ( 1586 )
江苏大学学报:医学版
　

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