Journal of Jiangsu University(Medicine Edition)

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Journal of Jiangsu University(Medicine Edition)

	2015 Vol.25 Issue.1 Published 2015-01-31

Article

	1	Effects of bisphenol A on ryanodine receptors in mouse testis and epididymis: role of estrogen receptors
		WANG Qiang1, WU Yi-rui1, ZHU Jing-gang1, JIN Min1, LU Rong-zhu1, XIAO Hang2
		Objective: To explore the possible effects of bisphenol A on ryanodine receptors(RyRs) in mouse testis and epididymis,the role of the two ERs was also investigated. Methods: Adult male wild type (WT), ERs knock out mice (αERKO and βERKO) were randomly divided into control and bisphenol A［100 μg/(kg·d) for 30 days via intragastric administration］ treatment groups．Quantitative real time PCR was used to examine RyR1, RyR2 and RyR3 genes of the testis and epididymis. Results: The mRNA expression of RyR1 and RyR3 were increased in bisphenol Atreated animals, however, RyR2 was evidently decreased. In the epididymis,the mRNA expression of RyR1, RyR2 and RyR3 were significantly increased(P<0.05).Bisphenol A upregulation of RyR1 of the epididymis mRNA expression was dependent on ERα,whereas bisphenol A regulation of RyR2 of the testis and RyR2, RyR3 of the epididymis appeared to be dependent on both ERα and ERβ. Conclusion: The ERs signalling was necessary for the regulation of the RyR channels mRNA expression by bisphenol A,which could affect the male reproductive function.
		2015 Vol. 25 (1): 1-4 [Abstract] ( 1168 ) [HTML 1KB] [ PDF 1040KB] ( 1638 )

	5	Sodium valproate had protective effect on rotenone induced injury in SH-SY5Y cells
		LI Yun-zi, QIU Jing, SUN Jing, GAO Jing
		Objective: To study the effect and the molecular mechanism of sodium valproate on rotenone induced injury in SH-SY5Y cells. Methods: After pretreatment of 100 μmol/L sodium valproate, SH-SY5Y cells were treated with 400 nmol/L rotenone; MTT assay was used to analyze cells viability; spectrophotometry was used to check mitochondrial complex Ⅰ activity; the expression of Acyl-CoA lysocardiolipin acyltransferase 1(ALCAT1), silent information regulator 1(SIRT1) and peroxisome proliferator activated receptor γ coactivator 1α(PGC-1α) were checked by Western blot; mRNA level of PGC-1α was analyzed by RT-PCR. Results: Pretreatment of 100 μmol/L sodium valproate for 3 h in SH-SY5Y cells could significantly increase cells viability, inhibit the expression of ALCAT1, elevate the activity of mitochondrial complex Ⅰ, increase the expression of SIRT1 and PGC-1α, but had no effect of mRNA level of PGC-1α. Conclusion: Sodium valproate had protective effect on rotenone induced injury in SH-SY5Y cells.
		2015 Vol. 25 (1): 5-9 [Abstract] ( 1289 ) [HTML 1KB] [ PDF 1033KB] ( 1863 )

	10	ZIC1 expression in colorectal cancer tissue and its effect on colon cancer cell proliferation
		WANG Jie-feng1, LI Peng-fei1, CAO Fang2, LU Wei-jie2, DING Hou-zhong2
		Objective: To investigate the expression of zinc finger of the cerebellum 1 (ZIC1) gene in the colorectal cancer tissue and its impact on the proliferation of colon cancer HCT8 cells. Methods: Tissue microarray blocks containing 192 colorectal cancer tumor specimens were conducted. Expression of ZIC1 in colorectal cancer tumor specimens and adjacent cancer tissues was analyzed using immunohistochemistry; the relationship between ZIC1 expression and clinical pathological parameters was analysed. The pcDNA3.1ZIC1 expression vector (experiment group) and pcDNA3.1 empty carrier group (negative control group) were constructed to transfect HCT8 cells, Western blotting and RTPCR were used to detect ZIC1 expression among the experiment group, negative control group and blank control group (no transfection cultivation).The changes of cells proliferation of three groups were determined by MTT. Results: The positive expression rate of ZIC1 in colorectal cancer tissue was higher than adjacent cancer tissues ( 41.1% vs 68.8%, P<0.05). The ZIC1 expression in colorectal cancer tissues was correlated with Dukes staging, degree of differentiation, infiltration depth and lymph node metastasis (P<0.05), whereas incorrelated with age and gender(P＞0.05). RTPCR and Western blotting showed the expression of ZIC1 in experiment group was significantly higher than that in negative control group and blank control group; MTT showed the cell proliferation in experiment group reduced markedly. Conclusion: ZIC1 might participate in the development and progression of colorectal cancer.
		2015 Vol. 25 (1): 10-13 [Abstract] ( 963 ) [HTML 1KB] [ PDF 1353KB] ( 1485 )

	14	Growth status of ovarian cancer cells in Matrigel three dimensional culture model
		CAI Guo-hui, HAN Yun-zhu, TANG Fu-shan, SONG Hong
		Objective: To establish the threedimensional(3D) culture model of ovarian cancer by means of Matrigel as scaffolds and to study the cellular morphology, function and proliferation . Methods: The morphology, distribution and viability of ovarian cancer cells in three dimensional culture were identified by fluorescence microscopy; Phalloidine/DAPI staining was used to assess the cellular morphology; The cell viability was measured by calcein AM staining; the cell proliferation was measured by measuring the content of DNA in cells; ELISA assay was used to assess the secretion status of associated factors(VEGFA , EGF and IGF1) in ovarian cancer cell. Results: Cells grown in Matrigel formed multicellular colonies and good ability and biocompatibility with the matrix. The associated factors (VEGFA, EGF and IGF1) in ovarian cancer cells assay indicated that there were significant differences of their secreation in three dimensional culture compared to two dimensional culture. Conclusion: Three dimensional culture of ovarian cancer cells in Matrigel matrix could maintain the cells morphology, function and proliferation better.
		2015 Vol. 25 (1): 14-18 [Abstract] ( 1266 ) [HTML 1KB] [ PDF 1408KB] ( 2635 )

	19	Establishment of AOM/DSS induced a mice model of colitis associated cancer
		ZHANG Yue, TIAN Jie, TIAN Xin-yu, TANG Xin-yi, YANG Jun, GENG Li-na, MA Jie, XU Hua-xi, WANG Sheng-jun
		Objective: To establish a mice model of colitis associated cancer. Methods: The colitis associated cancer was induced by a single intraperitoneal injection of 12.5 mg/kg azoxymethane (AOM) and stepped by four cycles oral administration of 2% dextran sodium sulpate (DSS). The occult blood was observed everyday. At the end of the fouth cycle, the mice were killed for observing the spleen, mesenteric lymph nodes, colon; colon tissues were harvested for mucosa morphological changes under light microscopy. Results: Compared to the control group, the spleens, mesenteric lymph nodes were enlarged and the tumorigenesis was found in distal colon in the experiment group; and there were a lot of inflammation cell infiltration and dysplasia in colon. Conclusion: Administration of AOM combined with DSS could induce a mice model of colorectal cancer in a short period.
		2015 Vol. 25 (1): 19-21 [Abstract] ( 1706 ) [HTML 1KB] [ PDF 1229KB] ( 2965 )

	22	Expression of PKM2 in bladder carcinoma and its effect of downregulation on biological characteristics of bladder carcinoma cell line T24
		GUO Ke, MO Nai-xin
		Objective: To investigate the effects and possible mechanism of PKM2 on the proliferation, invasion,migration of human bladder cancer cell lines T24 by using RNA interfering methods. Methods: The expression of PKM2 protein in 50 cases of bladder tumor tissues and 15 cases of normal bladder tissues was detected by immunohistochemistry.PKM2 siRNA and control siRNA were transfected to T24 cells by Lipofectamine 2000 respectively. The stable PKM2 siRNA and RNAiVector cell lines were obtained after screening and identification. Untransfected and transfected nonsense siRNA cells were used as blank control group and negative control group, the cells transfected PKM2 siRNA were as the experimental group. Real timeRCR and Western blot were used to measure the PKM2 expression at mRNA and protein levels. Cell proliferation rate were determined by MTT assay. Cell migration and invasion were determined by cell scratch experiment and Transwell invasive assays. Results: The positive expression of PKM2 protein in bladder cancer tissues was significantly higher than that of normal bladder tissues, and correlated with the pathological grade and clinical stage. In the PKM2 siRNA stable cell lines T24,the expression of PKM2 mRNA and protein were significantly decreased compared with these in blank control group and negative control group(P<0.05).The cell proliferation，the number of transmembrane cells and the ability of migration of PKM2 siRNA bladder cancer cell line were markedly reduced(P<0.05). Conclusion: The PKM2 overexpressed may play an essential role in the occurrence and development of bladder cancer, down regulation of PKM2 expression can inhibit the proliferation, migration and invasion of bladder cancer cell.
		2015 Vol. 25 (1): 22-26 [Abstract] ( 1589 ) [HTML 1KB] [ PDF 1446KB] ( 1455 )

	27	Construction of recombinant expression lentivirus vector carrying IκBα-DN and identification of its role in NFκB signaling pathway
		LU Yao, YAN Qin, LU Chun
		Objective: To construct the recombinant lentivirus carrying the dominantnegative construct of IκBα (IκBαDN), and identify the role in NFκB signaling pathway. Methods: The fragment of IκBαDN sequence from expression vector pMIGR1IκBαDN was cloned into the lentivirus vector pCDHCMVMCSEF1copGFP. The recombinant plasmid pCDHIκBαDN, packaging vector psPAX2 and envelope vector pMD2.G were cotransfected into 293 T cells. The transfection efficiency was determined by observing the expression of green fluorescent protein (GFP). The total of 293 T cells were transduced with LentivirusIκBαDN in the same multiplicity of infection (MOI) and IκBα protein was detected by Western blot. Next, LentivirusIκBαDN was transduced into 293 T cells transfected with NFκB luciferase reporter plasmid before and then the activity of NFκB was detected. Similarly, human umbilical vein endothelial cells(HUVECs) expressing Kaposis sarcomaassociated herpesvirus(KSHV) encoding viral FLICE inhibitory protein(vFLIP) were infected with LentivirusIκBαDN and the expression of IκBα protein was determined by Western blot. Results: The recombinant plasmid pCDHIκBαDN and the recombinant lentivirus carrying IκBαDN were constructed successfully in succession. The titer of LentivirusIκBαDN was 3×107 efu/mL. The expression of cytoplasmic IκBα protein in 293 T cells infected by LentivirusIκBαDN was significantly increased. Further, the result of luciferase reporter assay indicated that LentivirusIκBαDN inhibited the activity of NFκB. The same result was found in HUVECs expressing vFLIP and infected by LentivirusIκBαDN. Conclusion: The recombinant lentivirus containing IκBαDN was successfully constructed, and was able to infect 293 T cells and HUVECs effectively. Our date indicated that IκBαDN could increase the content of IκBα in cytoplasm, and further inhibit NFκB signaling.
		2015 Vol. 25 (1): 27-32 [Abstract] ( 1240 ) [HTML 1KB] [ PDF 964KB] ( 1846 )

	33	Intrathecal injection of TNFα antibodies attenuates bone cancer pain in rats
		LU Xiang-rong1, WANG Feng1, WEI Jing-rong2, ZHOU You-lang2, MIAO Xiu-hua3, XIAO Ying2, XU Wei-yuan3
		Objective: To investigate the analgesic effect of TNFα monoantibodies on the development of bone cancer pain in rats and its possible mechanism involved. Methods: Thirtytwo (n=32) normal female SpragueDawley rats weighting 180-200 g were randomly divided into 4 groups (n=8 for each group): sham operation group, bone cancer pain group, TNFα monoantibody group and vehicle group. The rat model of bone cancer pain was induced by injection of breast cancer cells (Walker 256) 10 μL (2×107cell) into the left proximal tibia bone marrow cavity. Sham operation group was injected by 10 μL normal saline. On the 11th day after inoculation, TNFα monoantibody group received intrathecal injection of 8 μL TNFα monoantibodies (etanercept) once daily for consecutive 7 days.The vehicle group received 8 μL intrathecal injection of normal saline solution for consecutive 7 days, while the rest two groups without intrathecal injection.Mechanical pain threshold was measured before inoculation and on day 1,4,7,10,14,17 after inoculation. On day 17 after inoculation, the left side dorsal root ganglion (DRG, L2 to L5) were harvested to detect the expression of voltagegated sodium channel NaV1.8. Results: Compared with sham operation group, the mechanical pain threshold began to decline on day 7 after inoculation and significantly decreased on day 10 after inoculation in bone cancer pain group (P<0.05). On day 17, NaV1.8 in the DRGs of bone cancer pain group expressed significantly higher than it in sham operation group (P<0.05); On day 11 the mechanical pain threshold increased dramatically in TNFα antibody group, reached peak at 2 h after drug injection and returned to baseline level at 8 h (P<0.01). The behavioristics change was similar to the first dose, but the increase of the mechanical pain threshold was decreased after repeated injection for 7 days. On day 17 the expression of NaV1.8 in TNFα monoantibody group was much lower than it in the control group (P<0.01). Conclusion: The TNFα monoantibody reduced bone cancer pain which may be through inhibiting the high expression of NaV1.8 in DRG.
		2015 Vol. 25 (1): 33-37 [Abstract] ( 1057 ) [HTML 1KB] [ PDF 863KB] ( 1645 )

	38	Effect of a novel indole-3-derivate indirubin on human HepG2 cells and underlying mechanism
		TAN De-fei1， GUO Wen-jie1， XIA Juan1， GUAN Ting-wei1， GAO Jing1，YAO Qi-zheng2
		Objective: To investigate the antitumor effects and possible mechanisms of a novel indole3derivate indirubin in vitro. Methods: Human HepG2 cells were exposed to different concentrations of indole3derivate A，cell proliferation was detected by MTT assay and cell colony formation of HepG2 cells treated with indole3derivate A was also observed. Then the nuclei changes were observed by staining with DAPI and cell apoptosis was detected by AnnexinV/PI staining.Meanwhile, mitochondrial membrane potential and intracellular free Ca2+ content were detected with JC1and Fluo3/AM staining, respectively. Moreover, the protein levels of Bcl2, Bax and activation of Caspase3,9 were examined by western blotting. Results: Indole3derivate A inhibited the proliferation and colony formation of HepG2 cells in a doseand timedependent way. Besides that, it was found that nucleus morphology was showing pyknosis and fragmentation and early apoptotic cells were significantly increased by AnnexinV/PI staining after indole3derivate A treatment.Moreover, western blotting showed indole3derivate A activated Caspase3,9, decreased the expression of Bcl2 and increased the expression of Bax in a dosedependent manner. In addition,indole3derivate A caused a loss of mitochondrial membrane potential and intracellular Ca2+ overloading.Conclusions: Indole3derivate A could significantly inhibit proliferation of HepG2 cells and the mechanisms might be related to mitochondria dysfunction and activation of mitochondriadependent apoptotic pathway.
		2015 Vol. 25 (1): 38-42 [Abstract] ( 927 ) [HTML 1KB] [ PDF 1502KB] ( 1644 )

	43	Metaanalysis of cerebrospinal fluid βamyloid42 in the progress of mild cognitive impairment
		ZHENG Yuan, WEN Zhong-min
		Objective: To evaluate the clinical value of cerebrospinal fluid (CSF) βamyloid42(Aβ42）in patients with mild cognitive impairment (MCI) on the progress of MCI. Methods: Computer retrieval was performed for CSF Aβ42 levels among stableMCI and progressiveMCI patients in PubMed, Cochrane Library, Ovid, CNKI, VIP Data, and Wanfang Data from January 2000 to December 2013. Metaanalyses were conducted to assess the Aβ42 levels in two groups. Publication bias between studies was estimated by Egger′s regression test and failsafe number(Nfs). The heterogeneity was explored by meta regression test. Results: Ten studies met inclusion criteria. Metaanalysis results showed CSF Aβ42 levels were significantly lower in progressiveMCI patients than those in stableMCI patients (SMD=-1.074, 95%CI=-1.299--0.848; Z=9.35, P<0.001). There was no significant publication bias tested by Egger′s regression test.The Meta regression test illustrated the variable of years of followup and year of publication were the main sources of heterogeneity. Conclusion: As a biomarker, low CSF Aβ42 levels in the CSF might help to predict the conversion from MCI to dementia.
		2015 Vol. 25 (1): 43-48 [Abstract] ( 760 ) [HTML 1KB] [ PDF 797KB] ( 1529 )

	49	Effect and mechanism of the proliferation and apoptosis of the MCF-7 cells on copper complexes with aromatic dldehyde derivatives in vivo and vitro
		YU Hao1, XIAO Xiu-di1, XU Xue-song1, LI Qiao-yu2
		Objective: To investigate the effect of the copper complexes with aromatic aldehyde derivatives,which was hereinafter referred to as aromatic aldehydecopper drug, on breast cancer cell(MCF7 cell line) in vivo and vitro and its mechanism of action. Methods: MCF7 cells were treated with different concentrations of the aromatic aldehydecopper drug,MTT assay was performed to determine cell ratio. Cell apoptosis were served by AnnexinV/PI flow cytometry. Western blotting was performed to evaluate the protein expression of p53,Bax and Bcl2. The subcutaneous transplantable tumor model of MCF7 cell in nude mice was established. The nude mice in each group received corresponding treated. The growth curves of the tumor were drawn and the tumor growth inhibition rates were calculated，and the toxic and side effort were observed too. Results: Aromatic aldehydecopper drug effectively inhibited the proliferation of MCF7 in a dose dependent manner, and the IC50 was 1.1 μmol/L. Aromatic aldehydecopper drug induced the apoptosis of MCF7 cells in a timedependent way(P<0.05), which was accompanied with upregulation of p53 and Bax expression, while Bcl2 expression was down regulated.In vivo,aromatic aldehydecopper effectively inhibited the growth of MCF7 tumor in a dose dependent manner(P＜0.05).There was no notable difference in the side effect. Conclusion: Aromatic aldehydecopper drug may inhibit the proliferation of MCF7 cells through inducing cell apoptosis, which should be related to the upregulation of p53 and Bax，while downregulated Bcl2.
		2015 Vol. 25 (1): 49-52 [Abstract] ( 832 ) [HTML 1KB] [ PDF 1062KB] ( 1522 )

	53	Expression of ZIC1 gene in breast cancer and its effect on the proliferation of MDA-MB-231 cell lines
		LI Peng-fei1, WANG Jie-feng1, CAO Fang2, YANG Zheng-fu2, DING Hou-zhong2
		Objective: To investigate the expression of zinc finger of the cerebellum 1 (ZIC1) gene in human breast cancer and its effect on proliferation of breast cancer MDAMB231 cell lines. Methods: Immunohistochemical technique was used to examine the expression of ZIC1 protein in 238 specimens of breast cancer and 40 adjacent cancer tissues. The relationship between ZIC1 protein expression and clinicopathological parameters of breast cancer was analyzed. The recombinant eukaryotic expression plasmid pcDNA3.1ZIC1 was constructed and transiently transfected into breast cancer MDAMB231 cell lines by lipofectamine(experiment group). For the following experiments, untransfected and pcDNA3.1 plasmid transfected cells were used as control. RTPCR and western blotting were applied to detect the expression of ZIC1 mRNA and protein in three groups. Cell proliferation was then detected by MTT assay. Results: The positive expression rate of ZIC1 protein in specimens of breast cancer was significantly lower than that in adjacent cancer tissues \[39.1% (93/238) vs 77.5% (31/40), P<0.05\]. The positive expression of ZIC1 protein was correlated with pathological classification, histological grade and lymph node metastasis. Compared with blank control group and negative control group, the expression of ZIC1 mRNA and protein level were positive in experiment group and cell proliferation ability was drastically repressed. Conclusion: Positive expression rate of ZIC1 protein was decreased in specimens of breast cancer. After transfection of ZIC1 gene, the growth of breast cancer MDAMB231 cell lines were inhibited, which suggest that ZIC1 gene may play an important role in the occurrence and development of breast cancer．
		2015 Vol. 25 (1): 53-56 [Abstract] ( 1415 ) [HTML 1KB] [ PDF 1458KB] ( 1627 )

	57	Meta analysis the association of polymorphisms in estrogen receptor α with male infertility
		LI Tian-fu1, ZHOU Qing1, ZHANG Cui2, JIANG Wei-jun2, WU Qiu-yue2, LI Wei-wei2, XU Hao-qin3, XIA Xin-yi1，2
		Objective: To analysis the relationship between two polymorphism genes of estrogen receptor α rs2234693 and rs9340799 and male infertility by Metaanalysis. Methods: We searched PubMed, EMABSE, CNKI and WanFang database, while 24 articles were concluded. Meta analysis was performed using Stata 11.0 software. Calculated the odds ratio and 95% confidence interval and evaluated the strength of the associations, sensitivity and publication bias. Results: For the rs2234693, there were significant association in the comparison of CC vs. TT (OR=0.72, 95% CI: 0.54-0.96), CT vs. TT (OR=0.74, 95% CI: 0.58-0.94) and (CC+CT) vs. TT (OR=0.73, 95% CI: 0.58-0.91) with male infertility. For rs9340799 polymorphism, increased risks were observed for the comparison of AA vs. GG (OR=1.67, 95% CI: 1.21-2.32) and AA vs. (GA+GG) (OR=1.63, 95% CI: 1.32-2.03). Conclusion: The rs2234693 CC genotype was associated with the decreased risk for male infertility; however, the rs9340799 AA genotype was associated with an increased risk for male infertility.
		2015 Vol. 25 (1): 57-61 [Abstract] ( 1097 ) [HTML 1KB] [ PDF 872KB] ( 1814 )

	62	Clinical utility of plasma APC and DCC genes promoter hypermethylation in the early diagnosis of lung cancer
		ZHU Yu-Min1, LI Jian1,YU LiChao2, ZHU LiHuan1, CHEN Ping1
		Objective: To evaluate clinical utility of the genes promoter hypermethylation of adenomatous polyposis coli (APC) and deleted in colorectal carcinoma (DCC) in plasma for the early diagnosis of lung cancer. Methods: A methylation specific PCR (MSP) was used to detect the status of APC and DCC promoter hypermethylation in plasma DNA from 70 lung cancer patients, 40 benign lung disease patients and 30 healthy volunteers. Serum carcinoembryonic antigen (CEA) was detected simultaneously and compared with the plasma APC and DCC genes methylation. Results: (1) The positive rates of plasma APC and DCC genes promoter methylation in lung cancer patients were 25.71% (18/70) and 35.71% (25/70), respectively, which were similar to the positive rate of serum CEA(37.14%). Whereas the positive rates of APC and DCC genes methylation in plasma of benign lung disease patients were 2.50% (1/40) and 7.50% (3/40), respectively. The two genes methylation in plasma was not found in healthy volunteers. There was a significant difference between patients with lung cancer and patients with benign lung disease in plasma APC and DCC genes promoter methylation (P＜0.05); (2) Combination of plasma APC and DCC genes methylation raised sensitivity to 51.43%, with 90.00% specificity for the diagnosis of lung cancer. Combined use of APC, DCC and CEA achieved sensitivity of 68.57%, with slight decrease of specificity (82.50%); (3)APC and DCC genes promoter methylation in lung cancer patients were not associated with the age, sex, smoking index, pathological types, the degree of tumor differentiation, clinical staging and lymph metastasis of patients with lung cancer. Conclusion: Plasma APC and DCC genes methylation might be two potential candidates as tumor markers of lung cancer. The detections of the two genes methylation may be a complementary tool for the early diagnosis of lung cancer.
		2015 Vol. 25 (1): 62-67 [Abstract] ( 1116 ) [HTML 1KB] [ PDF 856KB] ( 1764 )

	68	Expression of miR-93 in AML and association between miR-93 and miR-378
		TANG Qin1,2, YIN Jia-yu1, ZHU Xiao-wen1, ZHANG Ying-ying1, ZHOU Jing-dong1, DENG Zhao-qun3
		Objective: To investigate the expression of miR-93 in acute myeloid leukemia(AML) patients and the correlation between miR-93 and miR-378. Methods: Real-time quantitative PCR was performed to detect the expression level of miR-93 in AML patients. The clinical significance of miR-93 expression in AML was investigated. Results: miR-93 overexpression was identified in 14(16.7%) of 84 AML patients. The patients with miR-93 overexpression had lower white blood cell level than those without miR-93 overexpression (3.11×109/L versus 14.55×109/L, respectively,P=0.003).Compared with other karyotype classifications, the frequency of miR-93 overexpression in the intermediate ones was lower \[4/46（8.7%）versus 10/38（26.3%），P=0.041\].Spearman correlation analysis revealed positive association between the two miRNAs, with r=0.321 between miR93 and miR378. Conclusion: miR93 might serve as a diagnostic molecule with miR-378 in AML.
		2015 Vol. 25 (1): 68-71 [Abstract] ( 875 ) [HTML 1KB] [ PDF 780KB] ( 1361 )

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		2015 Vol. 25 (1): 85-87 [Abstract] ( 1045 ) [HTML 1KB] [ PDF 762KB] ( 2026 )

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