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Journal of Jiangsu University(Medicine Edition)

	2024 Vol.34 Issue.06 Published 2024-11-22

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		2024 Vol. 34 (06): 1- [Abstract] ( 5 ) [HTML 1KB] [ PDF 1329KB] ( 130 )

	461	Bioinformatics analysis of ferroptosis-related genes in patients with atherosclerosis in type 2 diabetes mellitus

		Objective： To explore the hub genes and potential mechanism of ferroptosis in the development of atherosclerosis (AS) in patients with type 2 diabetes mellitus (T2DM) based on bioinformatics. Methods： The datasets GSE20966 (T2DM) and GSE43292 (AS) were obtained from the GEO database. Differentially expressed genes (DEGs) were identified using the Limma R package. Heatmaps and volcano plots were drawn, and crossanalysis was performed to obtain DEGs associated with the two diseases. GO and KEGG enrichment analysis was performed to explore the biological functions of DEGs. Ferroptosis-related genes (FRGs) obtained from the FerrDb database were crosslinked, and hub genes were screened using LASSO regression and random forest analysis. ROC curves and validation sets GSE76895 (T2DM) and GSE28829 (AS) were used for verification. Finally, the gene-miRNA network was drawn. Results： A total of 606 DEGs were identified related to the T2DM and the AS datasets. Twenty potential genes were obtained by cross-analyzing with FRGs. Cyclin-depedent kinase inhibitors 1A (CDKN1A), poly ADPribose polymerase 8 (PARP8), phosphatidylethanolamine binding protein 1 (PEBP1), and progesterone receptor membrane component 1 (PGRMC1) were hub genes that affected AS in T2DM patients through ferroptosis. The area under the curve (AUC) of the ROC curves in the datasets GSE20966 and GSE43292 of the four genes was all greater than 0.7, which had diagnostic value. PEBP1 and PGRMC1 were significantly down-regulated in the validation sets GSE76895 and GSE28829. In addition, 13 miRNAs were closely associated with 4 hub genes. Conclusion： CDKN1A, PARP8, PEBP1 and PGRMC1 are involved in AS in T2DM patients through ferroptosis and may become new therapeutic targets. ［Key words］ferroptosis; type 2 diabetes mellitus; atherosclerosis; bioinformatics; machine learning
		2024 Vol. 34 (06): 461-468 [Abstract] ( 5 ) [HTML 1KB] [ PDF 15843KB] ( 131 )

	469	umbilical cord mesenchymal stem cell-derived small extracellular vesicles attenuate kidney damage in rats with diabetic nephropathy by inhibiting THBS1

		Objective: To investigate the effect of human umbilical cord mesenchymal stem cell derived small extracellular vesicles (hucMSC-sEVs) in attenuating the damage of diabetic kidney disease (DKD) in rats. Methods: HucMSCs were isolated and cultured, and hucMSC-sEVs were extracted from the culture media supernatant. The DKD rat model was established by high fat diet combined with injection of streptozocin, rats were randomly divided into control group, DKD group, and hucMSCsEVs group, with 6 rats in each group. The hucMSC-sEVs group received tail vein injection of hucMSC-sEVs 8 weeks after modeling, and kidney tissues were collected at week 24. Renal tissue pathology and fibrotic changes in rats were assessed using HE staining, PAS staining and Sirius Red staining. The expression of apoptosisrelated proteins Bcl-2 and Bax in kidney tissues was evaluated using Western bloting. Additionally, the expression levels of THBS1 and its receptors CD36 and CD47 in rat renal tissues were determined using histochemical staining, immunofluorescence staining, and Western bloting. Results: Compared with the control group, the DKD group exhibited significant pathological changes in the kidneys, such as thickening of the glomerular basement membrane and mesangial proliferation, dilation of renal tubules accompanied by vacuolar degeneration, and marked interstitial fibrosis with infiltration of inflammatory cells. The expression of the apoptosis marker Bax in kidney tissues showed a significant increase (P<0.05), while the expression of the anti-apoptotic marker Bcl-2 exhibited a significant decrease (P<0.01). The expression levels of THBS1 and its receptors CD36 and CD47 were also significantly elevated (P<0.001). In contrast, compared with the DKD group, the hucMSC-sEVs group demonstrated marked alleviation of renal tissue pathological damage and fibrosis. The expression of Bax significantly decreased (P<0.01), while the expression of Bcl-2 significantly increased (P<0.01). Additionally, the expression levels of THBS1 and its receptors CD36 and CD47 in the kidneys were significantly reduced (P<0.001). Conclusion: HucMSC-sEVs can significantly reduce the renal tissue injury in DKD, which may be related to the targeted inhibition of THBS1 expression. ［Key words］human umbilical cord mesenchymal stem cells; small extracellular vesicles; diabetic kidney disease; THBS1
		2024 Vol. 34 (06): 469-475,484 [Abstract] ( 6 ) [HTML 1KB] [ PDF 16565KB] ( 110 )

	476	Effects of CXCL14 on adipose tissue pyroptosis and aortic plaque of diabetic atherosclerotic mice

		Objective: To explore the effect of CXCL14 on pyroptosis of adipocytes and the formation of atherosclerosis in diabetic microenvironment. Methods: ① ApoE^-/- mice were intraperitoneally injected with streptozotocin to construct diabetes mellitus, and diabetic ApoE^-/- mice were fed with high fat diet for 20 weeks, and the diabetic atherosclerosis model (model group) was constructed, and control mice were only fed on a high-fat diet (AS group); ② Anti-CXCL14 short peptide was injected subcutaneously on the medial hind limb of diabetic mice (anti-CXCL14 group), and control diabetic mice were injected subcutaneously with normal saline only; ③ Adeno-associated virus (AAV) was injected in situ into posterior inguinal subcutaneous adipose tissue in diabetic mice to inhibit gastrointestinal dermatin (GSDMD)mediated pyroptosis, divided into: AAV-shscramble group, AAV-shGSDMD group, anti-CXCL14+AAV-shscramble group, anti-CXCL14+AAV-shGSDMDgroup. After 20 weeks of high-fat diet of the mice, the serum of the mice was collected under anesthesia, and the blood lipid levels (total cholesterol, triglycerides, LDL-C, and HDL-C) of the mice were analyzed by biochemical detection kit. The mice were sacrificed, and the epididymal adipose tissue was scanned by electron microscopy to observe the changes of fat cells. qRT-PCR was used to analyze the changes of pyroptosisrelated factors GSDMD, aspartate proteolytic enzyme-1 (Caspase-1), NLR family pyridine domain protein 3 (NLRP3) inflammasome, interleukin-1β (IL-1β) and inflammatory factor interleukin-6 (IL-6). The mouse aorta was isolated and extracted, and the relative area of atherosclerotic plaques was observed by HE staining and oil red O staining. Results: Compared with the AS group, the hypertrophy and number of adipocytes of adipose tissue in the epididymis in the model group decreased (P<0.01), the size of aorticplaque increased, the levels of total cholesterol, triacylglycerol and LDL-C were significantly increased, and HDL-C was significantly reduced. The levels of pyroptosisrelated factors and IL-6 in adipose tissue of epididymis were significantly increased (P<0.05), indicating that diabetes promoted pyroptosis of AS plaque and adipose tissue in mice. Injection of anti-CXCL14 short peptide could reduce the size of aortic plaque, improve blood lipid levels, and inhibit adipose tissue pyroptosis, which increases the number of fat cells. After GSDMD knockdown, the number of adipocytes increased and the area of aortic plaques decreased. However, after the injection of anti-CXCL14 immune peptide, there was no significant change in atherosclerosis in the AAV-shGSDMDgroup. Conclusion: Anti-CXCL14 can attenuate adipose tissue pyroptosis and alleviate the development of diabetic atherosclerosis. ［Key words］CXCL14; pyroptosis; adipose tissue; atherosclerosis; diabetic microenvironment
		2024 Vol. 34 (06): 476-484 [Abstract] ( 5 ) [HTML 1KB] [ PDF 16066KB] ( 132 )

	485


		2024 Vol. 34 (06): 485-489,506 [Abstract] ( 13 ) [HTML 1KB] [ PDF 19033KB] ( 136 )

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		2024 Vol. 34 (06): 490-496 [Abstract] ( 4 ) [HTML 1KB] [ PDF 4776KB] ( 133 )

	497	Identification of tumor microenvironment and intercellular communication-related signaling molecules of castration-resistant prostate cancer based on single cell RNA sequencing

		Objective： To explore intercellular communication-related signaling molecules in tumor microenvironment (TME) of castration-resistant prostate cancer (CRPC) and analyze their relationship with tumor occurrence and development. Methods： The main components of the TME in CRPC was analyzed using singlecell RNA sequencing (scRNA-seq) data (GSE137829) from CRPC patients found in the Gene Expression Omnibus (GEO) database. CellPhoneDB was used to examine interactions between different cells within the microenvironment, with a focus on revealing the connections between cancer-associated fibroblasts (CAFs) and malignant epithelial cells. CellChat was applied to analyze signaling molecules involved in communication between CAFs and malignant epithelial cells. Data from The Cancer Genome Atlas Prostate Adenocarcinoma (TCGA-PRAD) were integrated to identify signaling molecules closely related to the prognosis of CRPC patients. siRNA was used to knock down the expression of the signaling molecule Delta-like canonical Notch ligand 3 (DLL3) in CRPC cells, and the knockdown efficiency was validated by real-time quantitative PCR and Western blotting. The effects of DLL3 on the biological behaviors of CRPC cells, including proliferation, migration, and invasion, were investigated using CCK8 assays, EdU assays, and Transwell assays with Matrigel, respectively. Singlegene differential analysis, overrepresentation analysis (ORA), and gene set enrichment analysis (GSEA) were also performed to identify pathways enriched with DLL3-related differential expression genes. Results： The results of single-cell transcriptomic sequencing indicated that the main cellular components of the TME in CRPC include malignant epithelial cells, CAFs and immune cells. Close communication between CAFs and malignant epithelial cells was mediated by interaction-related signaling molecules, with 63 signaling molecules identified as closely related to the communication between CAFs and malignant epithelial cells. Univariate Cox regression analysis identified key signaling molecules, including tumor necrosis factorrelated ligand superfamily member 12 (TNFSF12) and DLL3, whose transcriptional expression were significantly associated with the prognosis of CRPC patients. Notably, high expression of DLL3 was significantly associated with poor prognosis in CRPC patients. In vitro experimental results demonstrated that knockdown of DLL3 significantly inhibited the growth, proliferation, migration and invasion capabilities of CRPC cells. KEGG and GSEA enrichment analyses indicated that differentially expressed genes related to DLL3 were significantly enriched in metabolic pathways. Conclusion： In the TME, CAFs and malignant epithelial cells communicate closely through intercellular signaling molecules. Among these signaling molecules, the expression of DLL3 is negatively correlated with cancer prognosis and affects the biological behavior of CRPC cells, suggesting that DLL3 may be a key signaling molecule in the malignant progression of human CRPC. ［Key words］castration-resistant prostate cancer; scRNA-seq; tumor microenvironment; cell communication; DLL3
		2024 Vol. 34 (06): 497-506 [Abstract] ( 7 ) [HTML 1KB] [ PDF 27995KB] ( 131 )

	507	Developmental toxicity and molecular mechanism of Di-(2-ethylhexyl) phthalate exposure on zebrafish embryos

		Objective： To study the effects of di-(2-ethylhexyl) phthalate (DEHP) on embryonic development of zebrafish and its potential molecular mechanism. Methods： Zebrafish embryos were randomly divided into 5 groups: blank control group, dimethyl sulfoxide group, and 8, 40, 200 μg/L DEHP groups, which were exposed to 96 hours post-fertilization (hpf). The mortality rate, hatching rate, malformation rate, heart rate, body length and the number of spontaneous movements of embryos were recorded. The activity of superoxide dismutase (SOD), glutathione catalase (GPx) and malondialdehyde (MDA) content in larvae were measured by enzymatic analysis. Real-time quantitative PCR was used to determine the expression of mRNA related to cell apoptosis, glucose transport, and the synthesis of proteins, fatty acids, and glycogen. Results： (1) Compared with the control group, the embryo/larvae mortality of zebrafish in 8 μg/L DEHP group was significantly increased (P＜0.01), and the larvae body length was greatly decreased (P＜0.05); in 40 and 200 μg/L DEHP groups, the mortality and malformation rate of zebrafish embryo/larvae were significantly increased (P＜0.01), and the hatching rate, heart rate, body length and the number of embryonic autonomous movement were greatly decreased (P＜0.05 or P＜0.01). (2) Compared with the control group, the activity of GPx in zebrafish larvae in 8 μg/L DEHP group was significantly decreased (P＜0.05); the activities of SOD and GPx in zebrafish larvae were significantly decreased and the content of MDA was greatly increased in 40 and 200 μg/L DEHP groups (P＜0.01). (3) Compared with the control group, the expression of Pi3k mRNA in glucose transport signaling pathway in 8 μg/L DEHP group was significantly up-regulated (P＜0.01); the mRNA expressions of Bax/Bcl-2, Caspase-3 and Ras were greatly up-regulated (P＜0.01), and the mRNA expressions of Akt and Pi3k in glucose transport signaling pathway were significantly up-regulated (P＜0.01), the mRNA expression of Erk1/2 was greatly down-regulated (P＜0.05 or P＜0.01), and the mRNA expressions of Mtor, Gsk-3 and GSK-3β in protein, fatty acid and glycogen synthesis signaling pathways were significantly up-regulated (P＜0.05 or P＜0.01) in 40 and 200 μg/L DEHP groups. Conclusion： DEHP may induce apoptosis, disrupt the expression of metabolism-related mRNA, cause oxidative damage, and thus affect the growth and development of zebrafish embryos, resulting in developmental toxicity. ［Key words］di-(2-ethylhexyl) phthalate; zebrafish embryo; developmental toxicity; mechanism of toxicity
		2024 Vol. 34 (06): 507-513,521 [Abstract] ( 8 ) [HTML 1KB] [ PDF 6540KB] ( 116 )

	514	Effect of bone marrow mesenchymal stem cell-derived exosomes on M1/M2 polarization of alveolar macrophages in rats with acute lung injury and its mechanism

		Objective: To investigate the effect of bone marrow mesenchymal stem cell-derived exosomes (BMSCs-Exos) on the M1/M2 polarization of alveolar macrophages NR8383 in rats with lipopolysaccharide (LPS)-induced acute lung injury and its potential mechanism. Methods: Bone marrow mesenchymal stem cells were isolated from Sprague-Dawley (SD) rats, and BMSCs-Exos were prepared. NR8383 cells were pretreated with 0.1 or 1 mg/mL BMSCs-Exos for 1 h, followed by induction with 1 μg/mL LPS for 48 h, respectively. ELISA and Western blotting were used to detect the inflammatory factor levels in the cell supernatant and the expression of intracellular polarization marker proteins, respectively. NR8383 cells were cultured alone or co-cultured with BMSCs, treated with 20 μmol/L exosome inhibitor GW4869, and induced with 1 μg/mL LPS for 48 h. Quantitative real-time PCR (qRT-PCR) and Western blotting were used to detect the intracellular miR-212-5p expression and the relative expression of IL-4Rα and p-STAT6 proteins, respectively. The binding site of miR-212-5p and IL-4Rα mRNA were predicted by bioinformatics and verified by luciferase assay. Results: BMSCs-Exos were successfully prepared. ELISA and Western blotting results showed that 1 μg/mL LPS induced for 48 h promoted the secretion of inflammatory factors (TNF-α, IL-1β, and IL-10) and M1 polarization of NR8383 cells. Pretreatment with BMSCs-Exos significantly reduced the contents of LPS-induced inflammatory factors TNF-α and IL-1β, upregulated the content of anti-inflammatory factor IL-10, inhibited M1 polarization and promoted M2 polarization of NR8383 cells, and the effect of 1 mg/mL BMSCs-Exos was significantly stronger than that of 0.1 mg/mL BMSCs-Exos. The cell co-culture experiment results showed that 1 μg/mL LPS induced for 48 h increased miR-212-5p levels and IL-4Rα and p-STAT6 protein expression in NR8383 cells. Co-culture with BMSCs greatly inhibited the effects of LPS on the above indicators, however the effect of BMSCs could be blocked by GW4869. Luciferase assay results indicated that miR-212-5p could bind to the 3′UTR of IL-4Rα mRNA and promote its protein expression. Conclusion: BMSCs-Exos could inhibit M1 polarization and promote M2 polarization of LPS-induced rat alveolar macrophage NR8383 through the miR-212-5p/IL-4Rα/ STAT6 pathway. ［Key words］extracellular vesicles derived from bone marrow mesenchymal cells; acute lung injury; alveolar macrophages; M2 polarization; miR-212-5p
		2024 Vol. 34 (06): 514-521 [Abstract] ( 6 ) [HTML 1KB] [ PDF 8376KB] ( 146 )

	522	Prediction of metastatic and recurrence in prostate cancer patients based on BMP-related differential gene in prostate cancer bone metastases

		Objective: To construct a prognostic risk model for predicting bone metastasis in prostate cancer (PCa) based on bioinformatics approach, and to investigate the effect of risk score on prognosis and immune infiltration. Methods: Clinical information and microarray expression data of PCa patients were downloaded from the Gene Expression Omnibus (GEO), and genes related to the bone morphogenetic protein (BMP) pathway were downloaded from the Molecular Signature Database (MSigDB). The differential expression genes (DEGs) of PCa bone metastasis samples and primary PCa samples were screened by differential analysis, and the intersection with BMP pathway-related genes was taken to obtain BMP-related DEGs, and the prognostic risk model was constructed by screening risk genes through LASSO regression analysis, and independent prognostic factors of PCa were screened by multifactorial regression analysis. The risk scores of patients were calculated according to the risk model and subsequently divided into two groups of high and low risk scores by corresponding score median values, and DEGs were identified for functional enrichment analysis to compare the differences in immune infiltration between high and low risk groups. Results: After the intersection of DEGs and BMP-related gene sets in 3 055 differentially expressed genes of PCa bone metastasis samples, 13 BMP-related DEGs were obtained. Four gene were screened by LASSO regression to construct a prognostic risk model, in which secreted frizzled-related proteins 2 (SFRP2), endoglin (ENG), and follistatin-like protein 1 (FSTL1) were risk genes and chordin like 1 (CHRDL1) was a protective gene. Kaplan-Meier analysis showed that metastasis-free survival and biochemical recurrence-free survival of patients in the high-risk group were significantly lower than that of patients in the low-risk group. Multifactorial regression analysis identified risk score as an independent prognostic factor for PCa bone metastasis, and patients in the high-risk group were more likely to develop bone metastasis and biochemical recurrence, and the risk score was positively correlated with the PSA value, the Gleason score, and the T stage. The DEGs of high-risk group were mainly involved in the activation of the WNT/BMP signaling pathway, and were significantly enriched in the proliferation of epithelial cells and extracellular matrix bioprocesses. GSEA analysis showed that, the differentially expressed genes were upregulated in the gene sets of mesenchymal transition, inflammatory response, angiogenesis, and multiple tumor metastasis gene sets. Immune infiltration analysis showed that PCa bone metastasis samples and patients in the high-risk group possessed a higher degree of immune cell infiltration, cancer associated fibroblasts (CAFs), macrophages, Estimate scores, stromal scores, and immune scores than the lowrisk group, and lower tumor purity than the low-risk group. Conclusion: The prognostic risk model of BMP-related DEGs constructed based on the LASSO regression analysis can effectively predict the occurrence of PCa bone metastasis, and the high-risk score is closely related to the prognosis and immune infiltration of PCa. ［Key words］prostate cancer; bone metastasis; BMP signaling pathway; prognostic risk model; immune invasion
		2024 Vol. 34 (06): 522-531,541 [Abstract] ( 6 ) [HTML 1KB] [ PDF 17116KB] ( 121 )

	532	Assessment of the effect of serum IL-17 levels on the efficacy of norethindrone acetate in the treatment of pain in patients with endometriosis

		Objective: To assess the effect of serum interleukin-17 (IL-17) levels for the effectiveness of norethindrone acetate in alleviating pain in patients with endometriosis (EMS). Methods: A total of 105 EMS patients (EMS group) and 80 healthy subjects (control group) were selected from the Fourth People′s Hospital of Zhenjiang from October 2020 to October 2022. Serum IL-17 levels were detected by ELISA in both two groups. The Visual Analogue Scale (VAS) was used to assess pain in the EMS group, and the correlation between pre-treatment serum IL-17 levels, r-AFS classification, and VAS pain scores was analyzed. EMS patients were treated with norethindrone acetate for 1 month. According to VAS pain scores before and after treatment, EMS patients were divided into effective group (n=65) and ineffective group (n=40). Logistic regression analysis was performed to evaluate the impact of pre-treatment serum IL-17 levels on the effectiveness of norethindrone acetate in alleviating EMS pain, and a ROC curve was plotted. Results: Before treatment, serum IL-17 level in EMS group was significantly higher than that in control group (P<0.05), and serum IL-17 level was positively correlated with r-AFS classification (r=0.764, P<0.05), and weakly correlated with VAS pain score (r=0.251, P<0.05). The serum IL-17 level in the effective group was significantly lower than that in the ineffective group before treatment(P<0.05). The increase of serum IL-17 level before treatment was a risk factor for the failure of norethindrone acetate to relieve pain in EMS patients (β=0.560, OR=1.751, P<0.05). The area under ROC curve was 0.814, with a sensitivity of 84.62% and a specificity of 72.50%. The optimal cutoff value was 62.36 pg/mL. Conclusion: Serum IL-17 levels are closely related to the development of EMS and could be a good predictor of the efficacy of norethindrone acetate on pain symptoms in EMS patients. ［Key words］endometriosis; pain; interleukin-17; norethindrone acetate
		2024 Vol. 34 (06): 532-535,549 [Abstract] ( 6 ) [HTML 1KB] [ PDF 3565KB] ( 120 )

	536	Preparation and evaluation of nebulized inhalation preparation of nitanib ethanesulfonate

		Objective： To study the optimized formulation of nidanib ethanesulfonate (NE) solution for inhalation and evaluate the formulation in vitro and in vivo. Methods： The prescription of NE solution was studied by optimizing the amount of the antioxidants, osmotic pressure regulators and pH value. The dynamic particle size of NE nebulized inhalation formulations was measured. The in vivo pharmacokinetics of NE nebulized inhalation formulations was determined by high performance liquid chromatography. Lung function, hydroxyproline (HYP), tumor necrosis factor-α(TNF-α), transforming growth factor-β1 (TGF-β1) were used as indicators to evaluate the therapeutic effect in vivo. Results： Optimized prescription was obtained: NE: 10 mg, sodium bisulfite: 4 mg, propylene glycol: 50 mg, water: 2 mL. The pH of the solution was adjusted to 4.0. The average particle size of NE nebulized inhalation was about 3.3 μm. The in vivo pharmacokinetic results in SD rats showed that the elimination half-life of NE inhalation preparation was 5.27 h. The bioavailability was significantly improved, reaching 63.71%. Pulmonary function parameters (tracheal contraction parameters, 50% tidal volume expiratory flow rate, respiratory rate) and lung dry/wet weight ratio, HYP, TNF-α in mice with pulmonary fibrosis model after inhalation administration had been significantly improved. Conclusion： NE inhalation can greatly improve lung function parameters and increase its bioavailability. ［Key words］nidanib ethanesulfonate; inhalation preparations; nebulized inhalation; pharmacokinetics; idiopathic pulmonary fibrosis
		2024 Vol. 34 (06): 536-541 [Abstract] ( 5 ) [HTML 1KB] [ PDF 4513KB] ( 127 )

	542	*Albendazole-loaded self microemulsifying drug delivery systems: formulation and in vitro-in vivo* evaluation**

		Objective： To establish an albendazole-loaded self-microemulsifying drug delivery system (ABZ-SMEDDS) to improve the solubility and oral bioavailability of the water poorly soluble drug. Methods： The composition of the self-microemulsion prescription was optimized by the establishment of pseudo-triphasic diagrams, then the particle size, drug loading, encapsulation rate, and micromorphology were determined. The in vitro drug release and cytotoxicity and cellular uptake were investigated on a Caco-2 cell model, and finally, the oral bioavailability of ABZ-SMEDDS in rats was investigated. Results： The optimal prescription composition of ABZ-SMEDDS is clove oil, polyoxyethylene hydrogenated castor oil RH40 and polyethylene glycol 400 in a mass ratio of 0.20∶0.64∶0.16. The particle size of ABZ-SMEDDS was (52.14±1.82)nm with a polydispersity index of 0.084±0.006. The drug loading and encapsulation efficiency were (36.60±1.20)mg/g and (98.12±2.2)%, respectively. The results of in vitro release and cell experiments showed that the dissolution rate of ABZ-SMEDDS in different dissolution media were greatly improved compared with ABZ, and it can promote the transmembrane absorption of drugs. Pharmacokinetic results in vivo showed that the relative oral bioavailability of ABZ-SMEDDS in rats was increased to 151.95% compared with the free drug. Conclusion： SMEDDS could be a potential carrier for the enhancement of drug dissolution and oral bioavailability of poorly water soluble drugs. ［Key words］albendazole; selfmicroemulsion; dissolution in vitro; cellular uptake; oral bioavailability
		2024 Vol. 34 (06): 542-549 [Abstract] ( 6 ) [HTML 1KB] [ PDF 10359KB] ( 124 )

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