椎间盘内注射碱性成纤维细胞生长因子对大鼠腰椎间盘针刺退变的预防作用

Abstract
Figure/Table
References
Related Citation (14)

Download: PDF (998 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract Objective: To observe the effect of prevention of SD rat lumbar disc acupuncture degeneration by the intradiscal injection of basic fibroblast growth factor (basic fibroblast growth factor, bFGF).Methods: Twenty-four SD rats were randomly divided into test group,control group and blank control group. There were 8 rats in each group. The posterior annulus fibrosus of L4/5 andL5/6 disc of test group were stabbed by 27-gauge hypodermic needle， then used the micro-injection syringe to inject 50 μL diluted bFGF. 50 μL saline was injected into the discs of control group. The discs of blank control group weren＇t stabbed. Eight weeks after surgery，all the rats were examined by MRI, the T2 weighted signal intensity of each group was measured under MRI. The states of disc degeneration were scored by Pfirrmann grading. Then all the rats were sacrificed and the discs were removed for pathological observation. Results： MRI showed signal intensity of nucleus pulposus in test group and blank control group was significantly higher than that in control group 8 weeks after surgery which resulted in a significant Pfirrmann score difference（P<0.05） among groups. Pathological examination showed the number of nucleus pulposus cells of test group and blank control group had no significant change and the fibers arranged in neat rows, meanwhile the number of control group nucleus pulposus cells significantly reduced, and the disordered arrangement of collagen fibers was also observed. Conclusion：Intradiscal injection of bFGF had a preventive effect for the SD rat intervertebral disc degeneration after posterior annulus fibrosus acupuncture.

Received: 10 January 2013

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors

Cite this article:

URL:

http://zzs.ujs.edu.cn/xbyxb/EN/ OR http://zzs.ujs.edu.cn/xbyxb/EN/Y2013/V23/I2/103

［１］Tsuji T, Matsuyama Y, Sato K,et al. Epidemiology of low back pain in the elderly: correlation with lumbar lordosis[J]. J Orthop Sci,2001, 6(4):307-311.

［２］Kent PM, Keating JL. The epidemiology of low back pain in primary care[J]. Chiropr Osteopat, 2005, 13:13.

［３］Fraser RD, Osti OL, VernonRoberts B. Intervertebral disc degeneration[J].Eur Spine J,1993,1(4):205-213.

［４］Pfirrmann CW, Metzdorf A, Zanetti M,et al.Magnetic resonance classification of lumbar intervertebral disc degeneration[J]. Spine,2001,26(1):1873-1878.

［５］Mwale F, Iatridis JC, Antoniou J. Quantitative MRI as a diagnostic tool of intervertebral disc matrix composition and integrity[J]. Eur Spine，2008,17 (Suppl 4): 432-440.

［６］Stelzeneder D, Messner A, Vlychou M,et al. Quantitative in vivo MRI evaluation of lumbar facet joints and intervertebral discs using axial T2 mapping[J]. Eur Radiol, 2011, 21(11):2388-2395.

［７］An HS,Masuda K, Inoue N. Intervertebral disc degeneration: biological and biomechanical factors[J]. J Orthop Sci,2006,11(5):541-552

［８］Pratsinis H, Kletsas D. PDGF, bFGF and IGF-I stimulate the proliferation of intervertebral disc cells in vitro via the activation of the ERK and Akt signaling pathways[J]. Eur Spine, 2007, 16(11):1858-1866.

［９］Thompson JP, Oegema TR Jr, Bradford DS. Stimulation of mature canine intervertebral disc by growth factors[J]. Spine,1991,16(3):253-260.

［10］Nagano T, Yonenobu K, Miyamoto S,et al. Distribution of the basic fibroblast growth factor and its receptor gene expression in normal and degenerated rat intervertebral discs[J].Spine,1995, 20(18):1972-1978.

［１１］Tolonen J, Grnblad M, Vanharanta H, et al. Growth factor expression in degenerated intervertebral disc tissue. An immunohistochemical analysis of transforming growth factor beta, fibroblast growth factor and platelet-derived growth factor[J]. Eur Spine J, 2006, 15(5):588-596.

［１２］Urban JP, Smith S, Fairbank JC. Nutrition of the intervertebral disc[J]. Spine,2004,29(23):2700-2709

［１３］Minamide A， Hashizume H, Yoshida M， et al. Effects of basic fibroblast growth factor on spontaneous resorption of herniated intervertebral discs. An experimental study in the rabbit[J]. Spine, 1999, 24(10): 940–945.

［１４］Zhao W, Chen B, Li X. et al. Vascularization and cellularization of collagen scaffolds incorporated with two different collagentargeting human asic fibroblast growth factors[J].J Biomed Mater Res A, 2007, 82(3):630-635.

［１５］叶冬平，梁伟国，戴丽冰，等.bFGF对人退变椎间盘髓核细胞影响的临床研究[J].细胞与分子免疫学杂志，2011，27（3）：317-319

［１６］Peng B, Hao J, Hou S, et al. Possible pathogenesis of painful intervertebral disc degeneration[J]. Spine, 2006,31(5):560-566.

［１７］Tolonen J, Gronblad M, Virri J, et al.Basic fibroblast growth factor immunoreactivity in blood vessels and cells of disc herniation[J]. Spine, 1995,20(3):271-276.

［１８］Kong MH, Do DH, Miyazaki M, et al. Rabbit model for in vivo study of intervertebral disc degeneration and regeneration[J]. J Korean Neurosurg Soc, 2008, 44(5):327-333.

［１９］Masuda K. Biological repair of the degenerated intervertebral disc by the injection of growth factors[J]. Eur Spine J,2008, 17 (Suppl 4):441-451.

［２０］Huang CY. Quantitative analysis of exogenous IGF-1 administration of intervertebral disc through intradiscal injection[J].Ｊ　Ｂiomech , 2012,45(7):1149-1155.

[21]Kawakami M, Matsumoto TH. Hashizume H， et al. Osteogenic protein(osteogenic protein-1/bone morphogenetic protein-7) inhibits degeneration and painrelated behavior induced by chronically compressed nucleus pulposus in the rat[J].Spine, 2005,30(17):1933-1940.

\[22]Walsh AJ, Bradford DS, Lotz JC. In vivo growth factor treatment of degenerated intervertebral discs[J]. Spine, 2004,29(2):156-163.