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Regulation of carbon monoxide-releasing molecule-2 on inflammatory responses in nervous system of septic mice |
(Department of Burns and Plastic Surgery,the Affiliated Hospital of Jiangsu University, Zhenjiang Jiangsu 212001,China) |
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Abstract Objective: To investigate the regulation effects of exogenous carbon monoxide-releasing molecule-2(CORM-2)on the nervous system in septic mice. Methods: To make standard cecal ligation and puncture(CLP)models,48 C57BL/6 mice were randomly divided into 4 groups: sham group,CLP group,CORM-2 intervention group and Carbachol group. The animals were sacrificed at 24 hours after experimental manipulation. Serum and brain tissue samples were collected,the expression of TNF-α,IL-6,neural expression of anti-inflammatory neurotransmitter acetylcholine(ACh),and the acetylcholinesterase(AChE)activity of brain tissues. To measure the wet-to-dry weight ratio of brain tissues and the pathology changes of brain tissues were also observed by HE dyeing. Results: Compared with the CLP mice,tissues injuries,pro-inflammatory cytokines(TNF-α and IL-6)expressions were markedly down regulated in CORM-2 and Carbachol intervention groups; while the concentration of neurotransmitter ACh were increased,and the ac tivity of AChE were inhibited in CORM-2 and Carbachol intervention mice. Conclusion: Taken together,the data of this study showed that CORM-2 intervention markedly inhibited inflammatory responses in the nervous system,indicating that the CORM-2 may play an important roles in the treatment of sepsis,and will be useful for the clinical treatment of critically ill patients in the future.
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