小鼠深静脉血栓实验模型的制备

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Abstract Objective： To prepare the experimental animal model of deep vein thrombosis(DVT) in mice. Methods： Fiftysix C57BL/6J mice were randomly divided into five groups: normal control group, sham operation group, operation group 1, operation group 2 and endotoxin+operation group. In operation group 1, 90% of inferior vena cava were ligated. The sham operation group performed the same operation except ligation. Endotoxin+operation group and operation group 2 were intraperitoneally injected with endotoxin and equal volume of normal saline 2 hours before surgery, and the other operations were the same as operation group 1. The general situation of the mice before and after the inferior vena cava stenosis was evaluated. The images of the structures and vessels around the inferior vena cava were obtained by Doppler ultrasonography before and immediately after the operation. The length, weight and percentage of thrombosis were determined 48 h after the operation as the quantitative indexes to evaluate the DVT model. Results： The total mortality after inferior vena cava stenosis was 25.0% and the success rate was 70.8%. Immediate postoperative ultrasound showed that there was still venous blood flow through the ligation site. No thrombus was observed in the normal control group and the sham operation group, and there was no significant difference in the size of thrombus between operation group 1 and operation group 2 （P>0.05). Compared with the operation group 2, the length of thrombus in the endotoxin+operation group was significantly increased （P<0.05), and the weight of thrombus was increased, but the difference was not significant （P>0.05). Conclusion： The model of DVT in mice prepared in this study has good operability, validity and practicability.

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Received: 30 May 2022

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http://zzs.ujs.edu.cn/xbyxb/EN/ OR http://zzs.ujs.edu.cn/xbyxb/EN/Y2022/V32/I05/398

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