穿心莲内酯对人乳腺癌细胞迁移和侵袭的抑制作用

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Abstract Objective:To investigate the effect and mechanism of Andrographolide (Andro) on the metastasis and invasion of human breast cancer MDA-MB-231 cells. Methods: MDA-MB-231 cells were treated with Andro(0,5,10, 20,40 and 80 μmol/L)for 24 h. MTT assay was used to detect cell proliferation.Wound healing and Transwell experiment were applied to detect cell migration and invasion ability.Reverse transcription polymerase chain reaction were applied to detect uncoupling protein 2 (UCP2) mRNA expression.The protein expressions of UCP2 and pyruvate dehydrogenase (PDH) were detected by Western blotting. Lactic acid kit was used to detect lactic acid content. JC-1 staining and laser confocal microscope were used to detect mitochondrial membrane potential (MMP). Results: Compared with the control group, Andro significantly inhibited the proliferation of MDA-MB-231 cells in a concentration-dependent manner. Compared with 0 μmol/L, the metastasis and invasion of MDA-MB-231 cells in the 10, 20, and 40 μmol/L groups were inhibited (P<0.05 or P<0.01); UCP2 mRNA and protein expression were downregulated (P<0.05 or P<0.01), MMP was increased, PDH protein expression was up-regulated, and intracellular lactate content was decreased (P<0.05 or P<0.01). Conclusion: Andro may inhibit the metastasis and invasion of MDA-MB-231 cells by inhibiting the glycolysis of breast cancer cells and altering energy metabolism, which may be related to inhibition of UCP2.

Received: 30 June 2021

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	Articles by authors
	FU Hexiu1
	LU Beibei1
	CHEN Qiuyun2
	GAO Jing1

Cite this article:

FU Hexiu1,LU Beibei1,CHEN Qiuyun2等.

Inhibitory effect of Andrographolide on the metastasis and invasion of human breast cancer cells

[J]. Journal of Jiangsu University(Medicine Edition), 2022, 22(1): 32-37.

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http://zzs.ujs.edu.cn/xbyxb/EN/ OR http://zzs.ujs.edu.cn/xbyxb/EN/Y2022/V22/I1/32

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