EUROPattern全自动免疫荧光核型和滴度判读系统检测抗核抗体的应用评价

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Abstract Objective: To evaluate the clinical application of antinuclear antibody (ANA) patterns and titer using the automated indirect immunofluorescence interpretation system based on EUROPattern Suit technology. Methods: A total of 202 serum samples were selected from Suzhou Municipal Hospital, including 177 patients with autoimmune diseases and 25 healthy controls. The antinuclear antibodies of two groups were determined by Sprinter XL automatic immunofluorescence analyzer, and then the ANA patterns and titer were evaluated using the EUROPattern Suit automated immunofluorescence interpretation system and traditional visual interpretation method. Results: ① The positive rates of two methods were respectively 85.6% and 86.1% , and there were no significant difference between two methods(P=0.886); The performance verification indicators, such as sensitivity, specificity, accuracy, positive predictive value and negative predictive value were compared, and none of the differences were significantly (P>0.05). ② It was shown that there were 169 positive cases and 24 negative case at the same time detected in two methods, the overall coincidence rate was 95.5% and the Kappa value of the consistency checking was 0.816. ③ The overall concordance rate of ANA patterns distribution in two methods was 85.1%. The concordance rates of different fluorescence patterns were respectively nuclear dots (100%),centromeres (93.8%), homogeneous (91.7%), nucleolar (88.5%), cytoplasmic (84.6%), speckled and mixed patterns (80.0%). ④ The concordance rates of ANA titer of every pattern in two methods were higher , ranging from 81.8% to 100.0%, except for the mixed pattern. Conclusion: Compared with the traditional visual interpretation method, the EUROPattern Suit automated fluorescence interpretation system had a higher sensitivity, specificity and concordance rate in interpreting the ANA pattern and titer. This assay can meet clinical requirements.

Received: 22 February 2019

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	Articles by authors
	XU Hong-xing
	LIU Yi
	ZHU Qin-fang
	XU Wei-dong

Cite this article:

XU Hong-xing,LIU Yi,ZHU Qin-fang等.

The clinical evaluation of antinuclear antibody patterns and titer using automated indirect immunofluorescence interpretation system based on EUROPattern Suit technology

[J]. Journal of Jiangsu University(Medicine Edition), 2020, 30(02): 165-168.

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http://zzs.ujs.edu.cn/xbyxb/EN/ OR http://zzs.ujs.edu.cn/xbyxb/EN/Y2020/V30/I02/165

［1］Wieser M,PohlaGubo G,Hintner H.Antinuclear antibodies (ANA) diagnostic value of different methods for screening and differentiation\[J\].Clin Appled Immunol Rev, 2001,1(3):201-206.

［2］Nossent H,Rekvig OP.Antinuclear antibody screening in this new millennium: farewell to the microscope\[J\].Scand J Rheumatol,2001,30(3):123-128.

［3］吴斌,巫志宇,陈鸣.自身抗体检测在自身免疫性疾病筛查中的临床应用分析\[J\].现代医药卫生,2014,30(3):407-408.

［4］Ghirardello A,Bendo R,Rampudda ME,et al.Commerical blot assay in the diagnosis of systemic rheumatic diseases\[J\].Autoimmun Rev,2009,8(8):545-549.

［5］吴秀风,陈惠娟,曾勇彬,等.三种抗核抗体检测方法的比较\[J\].中国卫生标准管理,2016,7(18):151-152.

［6］高启健,向波,刘忠民.抗核抗体两种检测方法在系统性红斑狼疮中的诊断性能比较\[J\].实用医学杂志,2017,33(9):1498-1502.

［7］宋月芹,张红斌,李雪莲. 两种检测抗双链DNA抗体方法在系统性红斑狼疮诊断中的价值比较\[J\].现代实用医学, 2017，29(4): 505-506.

［8］Arbuckle MR,McMican MT,Rubertone MV，et al.Development of autoantibodies before the clinical onset of systemic lupus erythematosus\[J\].N Engl J Med,2003,349(16):1526-1533.

［9］王兰兰.自身抗体检测的应用与质量保障原则\[J\].中华检验医学杂志,2005,28(10):987-990.

［10］Bizzaro N,Antico A,Platzgummer S,et al.Automated antinuclear immunofluorescence antibody screening:A comparative study of six computeraided diagnostic systems\[J\].Autoimmun Rev, 2014,13(3):292-298.

［11］Melegari A,Bonagur C,Russo A,et al.A comparative study on the reliability of an automated system for the valuation cellbased indirect immunofluorescence\[J\].Autoimmun Rev, 2011,11(10):713-716.

［12］汤春园,陶瑕.抗核抗体、抗双链DNA抗体研究与实验室检测的进展\[J\].内科,2008,3(3):421-423.

［13］Egerer K,Roggenbuck D,Hiemann R,et al.Automated evaluation of autoantibodies on human epithelial2 cells as an approach to standardize cellbased immunofluorescence tests\[J\].Arthritis Res Ther,2010,12(2):R40.