小鼠实验性干燥综合征模型的建立

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Abstract Objective: To establish experimental Sjgren syndrome(ESS) model by immunizing with salivary gland protein. Methods: C57BL/6 mice were immunized with salivary gland protein to establish ESS mouse model. The saliva flow rate was detected. Serum levels of autoantibodies was detected by ELISA kit. In addition, the pathological changes in salivary gland were observed by HE straining. Results: Compared with control group, the saliva flow rate was reduced in ESS mice(P<0.05), autoantibodies against salivary gland, antiM3R antibodies and antinuclear antibodies were significantly increased(all P<0.05). ESS mice exhibited bigger cervical lymph nodes and submandibular glands, and plenty of lymphocytes were infiltrated into salivary glands. Conclusion: The ESS model was successfully established by immunizing C57BL/6 mice with salivary gland protein.

Received: 06 May 2019

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http://zzs.ujs.edu.cn/xbyxb/EN/ OR http://zzs.ujs.edu.cn/xbyxb/EN/Y2019/V29/I05/390

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