|
|
Application of FAL medical glue in establishing the human gastric cancer tissue mass orthotopic transplantation model |
ZHANG Jie1,2, JIA Li-juan2, LIANG Bing2, BU Xue-feng1 |
(Department of General Surgery,the Affiliated People′s Hospital of Jiangsu University,Zhenjiang Jiangsu 212002;2.School of Clinical Medicine,Jiangsu University,Zhenjiang Jiangsu 212013,China) |
|
|
Abstract Objective: To establish nude mouse orthotopic transplantation model of human gastric cancer tissue mass by FAL medical glue for the study of pathophysiological process of gastric cancer and experimental study to provide an ideal animal model. Methods: Transplanted gastric cancer tissue mass was constructed through repeatedly subcutaneously inoculating and passaging human gastric cancer cell line SGC-7901 in BALB/C nude mouse, and pasted orthotopically on the greater curvature by FAL medical glue. The orthotopic transplantation tumor growth characteristics, and success rate of transplantion and metastasis rate were examined. Results: Orthotopic transplantation tumor rate was 100% by this way. As the extension of feeding time, lymph node metastasis, liver adhesion rate increased gradually. Conclusion: Using FAL medical glue can safely and conveniently establish human gastric cancer nude mouse orthotopic transplantation tumor model, and simulated gastric cancer clinical development as much as possible.
|
Received: 10 February 2014
|
|
|
|
[1]陈云逸.胃癌动物模型研究进展[J].肿瘤基础与临床, 2006,19(1):75-76.[2]Furukawa T, Kubota T, Watanabe M, et al. Differential chemosensitivity of local and metastatic human gastric cancer after orthotopic transplantation of histologically intact tumor tissue in nude mice[J].Int J Cancer,1993,54(3):397-401.[3]Yamaguchi K, Ura H, Yasoshima T,et al. Liver metastatic model for human gastric cancer established by orthotopic tumor cell implantation[J]. World J Surg,2001,25(2):131-137.[4]汪芳裕,朱人敏,王琳,等. 裸鼠人胃癌原位移植模型的建立[J] .癌症,1997,16 (5) :343-344.[5]Shi J, Wei PK, Zhang S,et al.OB glue paste technique for establishing nude mouse human gastric cancer orthotopic transplantation models[J].World J Gastroenterol,2008, 14 (30):4800-4804.[6]田霞, 卢永顺. 福爱乐医用胶(FAL)及其应用[J]. 国际外科学杂志, 2006, 33(1): 74-75.[7]吴细丕,钱林法. 实验动物与肿瘤研究[M].北京:中国医药科技出版社,2000:190-199.[8]Illert B, Otto C, Braendlein S, et al. Optimization of a metastasizing human gastric cancer model in nude mice[J]. Microsurgery,2003,23(5):508-512.[9]陈亚琳,魏品康,许玲,等. 采用OB 胶粘贴法建立人胃癌裸鼠原位种植转移模型[J].癌症,2005,24(2):246-248.[10]李莹杰,贺岩,王齐敏,等.优化胶粘贴法建立裸鼠胃癌原位种植模型[J].中国实验动物学报,2007,15(5):369-371.[11]夏穗生, 田霞, 卢永顺. 新一代喷涂型福爱乐医用胶:基础研究[J].临床外科杂志, 2003, 11(2) : 120-121. |
|
|
|