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| Application of FAL medical glue in establishing the human gastric cancer tissue mass orthotopic transplantation model |
| ZHANG Jie1,2, JIA Li-juan2, LIANG Bing2, BU Xue-feng1 |
| (Department of General Surgery,the Affiliated People′s Hospital of Jiangsu University,Zhenjiang Jiangsu 212002;2.School of Clinical Medicine,Jiangsu University,Zhenjiang Jiangsu 212013,China) |
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Abstract Objective: To establish nude mouse orthotopic transplantation model of human gastric cancer tissue mass by FAL medical glue for the study of pathophysiological process of gastric cancer and experimental study to provide an ideal animal model. Methods: Transplanted gastric cancer tissue mass was constructed through repeatedly subcutaneously inoculating and passaging human gastric cancer cell line SGC-7901 in BALB/C nude mouse, and pasted orthotopically on the greater curvature by FAL medical glue. The orthotopic transplantation tumor growth characteristics, and success rate of transplantion and metastasis rate were examined. Results: Orthotopic transplantation tumor rate was 100% by this way. As the extension of feeding time, lymph node metastasis, liver adhesion rate increased gradually. Conclusion: Using FAL medical glue can safely and conveniently establish human gastric cancer nude mouse orthotopic transplantation tumor model, and simulated gastric cancer clinical development as much as possible.
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Received: 10 February 2014
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2014, Vol. 24 
