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| Relationship between HBV basic core promoter coexistent mutation of 1762/1764 sites and viral genotypes or HBeAg seroconversion |
| XU Jun1， ZHANG Yuan-hai2， ZHENG Zhong-wei1， WU Jian-ye1， GE Guo-hong2， SHAO Jiang-bo2 |
| (1.Department of Digestive Diseases, the Third People′s Hospital of Changzhou, Changzhou Jiangsu 213000； 2. Department of Infectious Diseases, the Third People′s Hospital of Zhenjiang, Zhenjiang Jiangsu 212002, China) |
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Abstract Objective: Based on the results of the incidence rates of the basic core promoter (BCP) coexistent mutation of 1762/1764 sites and the expression of hepatitis B virus (HBV) e antigen (HBeAg) and its antibody in chronic hepatitis B(CHB) patients with common viral genotypes in China, to investigate the relationship between the coexistent mutation and both of viral genotypes and HBeAg seroconversion in the natural course of chronic HBV infection. Methods: The sera of 168 CHB patients randomly selected with no previous history of antiviral therapy were detected. Their viral genotypes and coexistent mutation were determined by probe-specific real-time polymerasechain reaction (PSRT-PCR) that applied TaqMan MGB probe which had been designed and made exactly in past time; and HBeAg and its antibody(HBeAb) titer were measured by chemiluminescence microparticle immunoassay(CMIA). Results: The coexistent mutation in BCP 1762/1764 sites was significantly more frequent in genotype C HBV patients than that in genotype B patients (70.65% vs 29.17%, P=0.000). The wild genotype B group had a significantly higher proportion of HBeAg-negative HBV patients than the coexistent mutation genotype B group (73.53% vs 28.57%, P=0.004) and the wild genotype C group (73.53% vs 22.22%, P=0.000); the coexistent mutation genotype C group had a significantly higher proportion of HBeAg-negative HBV patients than the wild genotype C group(63.08% vs 22.22%, P=0.000) and the coexistent mutation genotype B group(63.08% vs 28.57%, P=0.018). In all HBeAg-negative patients, the wild genotype B group had a more higher HBeAbpositive rate than the coexistent mutation genotype B group(84.00% vs 0.00%, P=0.003) and the wild genotype C group (84.00% vs 16.67%, P=0.004), while there was no significant difference between the coexistent mutation genotype C group and the wild genotype C group(19.51% vs16.67%, P=1.000) and the coexistent mutation genotype B group(19.51% vs 0.00%, P=1.000). Conclusions: During the natural course of chronic HBV infection in China, the BCP coexistent mutation of 1764/1762 sites was more common in patients with genotype C, compared with genotype B; and the thorough HBeAg seroconversion occurred more frequently in wild genotype B HBeAg-negative patients, with HBeAb-positive; while the halfway HBeAg seroconversion occured in coexistent mutation genotype C HBeAg-negative patients, without HBeAb-positive. ［Key words］hepatitis B virus; basic core promoter; mutation; genotype; hepatitis B e antigen seroconversion
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