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| Effects of postconditioning with sevoflurane on iNOS/NO pathway in rats with acute lung injury induced by lipopolysaccharide |
| YANG Fen, ZHAO Jian-hua |
| (Department of Anesthesia, Suzhou Municipal Hospital, Suzhou Jiangsu 215002, China) |
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Abstract Objective: To investigate the effects of postconditioning with sevoflurane on inducible nitric oxide synthase(iNOS)/nitric oxide(NO) pathway in rats with acute lung injury induced by lipopolysaccharide. Methods: Thirty rats were randomly divided into 5 groups(n=6)，NS group and LPS group, rats received intratracheal instillation of normal saline(1 mL/kg) and lipopolysaccharide(5 mg/kg), respectively; sevoflurane groups(S 0.5, 1, 2 h), 4 h after lipopolysaccharide intratracheal instillation(i.e. after the occurrence of acute lung injury), rats received sevoflurane inhalation(2.4%) for 0.5, 1, 2 h, respectively, simulating a postconditioning scheme. Lung tissue samples were harvested 6 h after normal saline or lipopolysaccharide intratracheal instillation. Histological examination by light microscopy was performed; iNOS expression and NO concentration in lung parenchyma were measured by immunohistochemistry and with NO Assay Kit. Results: Compared with the NS group, iNOS expression and NO concentration in the LPS group were significantly increased(P<0.01), with more severe lung injury. Compared with the LPS group, iNOS expression and NO concentration in S groups were greatly reduced(P<0.01 or P<0.05), with attenuated lung injury, especially in S1h and S2h groups. Conclusion: Postconditioning with sevoflurane could attenuate lipopolysaccharideinduced acute lung injury in rats through inhibiting iNOS/NO pathway activation and reducing the inflammatory response. ［Key words］sevoflurane; lipopolysaccharide; acute lung injury; postconditioning; inducible nitric oxide synthase; nitric oxide
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