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Analysis of aminoglycoside modifying enzyme genes and 16S rRNA methylase genes in drugresistant Escherichia coli strains |
YUAN Hong-yan1,2, Guo Bin-fang2, YIN Jing-ping1, SUN Qiang1, GAO Jing-jing1, SU Zhao-liang1, WANG Seng-jun1, XU Hua-xi1, MI Zu-huang3 |
(1. School of Medical Science and Laboratory Medicine, Jiangsu University, Zhenjiang Jiangsu 212013; 2.Blood Transfusion Department, the Second People′s Hospital of Changzhi, Changzhi Shanxi 046000; 3.Wuxi Clone GenTech Institute,Wuxi Jiangsu 214026, China)
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Abstract Objective: To analyze aminoglycoside modifying enzyme genes and 16S rRNA methylase genes in drugresistant Escherichia coli(DRECO)strains, and investigate the aminoglycoside resistance mechanisms. Methods: The expressions of aminoglycoside modifying enzyme genes and 16S rRNA methylase genes in 20 DRECO strains were assayed by polymerase chain reaction(PCR); and positive PCR products were sequenced by PCR direct automated fluorescence method. Results: In the 20 DRECO strains,18 strains expressed aminoglycoside modifying enzyme genes, the positive rate of aac (3)Ⅱ, aac (6′)Ib, ant (3″)I, aadA4/5 and aph (3′)I were 25%, 25%, 5%, 65% and 55%, respectively, only 1 strains expressed rmtB type 16S rRNA methylase gene, the positive rate was 5%, and the rest genes expressions were not detected. Conclusion: The aadA4/5 and aph (3′)I, not 16S rRNA methylase gene, were closely related with the DRECO aminoglycoside resistance.
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Received: 01 April 2013
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